3.1.1.31: 6-phosphogluconolactonase

This is an abbreviated version!
For detailed information about 6-phosphogluconolactonase, go to the full flat file.

Reaction

6-phospho-D-glucono-1,5-lactone
+
H2O
=
6-phospho-D-gluconate

Synonyms

lactonase, phosphoglucono-, phosphogluconolactonase, 6-phosphoglucono-gamma-lactonase, 6-phospho-D-glucose-delta-lactone hydrolase, 6-PGL, PGL, 6PGL, glucose-6-phosphate dehydrognease-6-phosphogluconolactonase, G6PD-6PGL, Glc6PD-6PGL, 6-phosphogluconolactonase, 6-phosphonogluconolactonase, 6PGL-G6PDH, Pf6PGL, YbhE protein, glucose-6-phosphate dehydrogenase-6-phosphogluconolactonase, Xoo2316, PGL1, PGL2, PGL3, PGL4, PGL5, GluPho, PglA, 6-phosphogluconolactonase 3, PF14_0511, OG1RF_11582, GL50803_8682

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.31 6-phosphogluconolactonase

Purification

Purification on EC 3.1.1.31 - 6-phosphogluconolactonase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2',5'-ADP-Sepharose
-
His-tagged YbhE protein
-
His-Trap nickel affinity column chromatography and Superdex 75 gel filtration
-
Ni-NTA His-Bind resin chromatography and UNO6 Q column chromatography
-
purified to 95% homogeneity in a single step with a Co-NTA column
recombinant 6PGL
recombinant G6PD-6PGL
-
recombinant Glc6PD-6PGL
-
the dimer undergoes dissociation at low concentrations, and the monomeric state is catalytically inactive. The enzyme maintains 100% of its activity when it is incubated at a concentration of 50 microg/ml
to 95% homogeneity in a single step with an NTA column
-