3.1.1.31: 6-phosphogluconolactonase
This is an abbreviated version!
For detailed information about 6-phosphogluconolactonase, go to the full flat file.
Reaction
Synonyms
6-PGL, 6-phospho-D-glucose-delta-lactone hydrolase, 6-phosphoglucono-gamma-lactonase, 6-phosphogluconolactonase, 6-phosphogluconolactonase 3, 6-phosphonogluconolactonase, 6PGL, 6PGL-G6PDH, G6PD-6PGL, GL50803_8682, Glc6PD-6PGL, glucose-6-phosphate dehydrogenase-6-phosphogluconolactonase, glucose-6-phosphate dehydrognease-6-phosphogluconolactonase, GluPho, lactonase, phosphoglucono-, OG1RF_11582, PF14_0511, Pf6PGL, PGL, PGL1, PGL2, PGL3, PGL4, PGL5, PglA, phosphogluconolactonase, Xoo2316, YbhE protein
ECTree
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Crystallization
Crystallization on EC 3.1.1.31 - 6-phosphogluconolactonase
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by the hanging-drop method, 6PGL in complex with two ligands, 6-phosphogluconic acid and citrate, at 2.2 A and 2.0 A resolution, respectively. Structures of the two complexes and of the apo form are almost identical. Both complexes belong to space group P21 and have two molecules in the asymmetric unit, related by a pure translation. PGL in complex with 6-phosphogluconic acid has unit cell parameters of a = 49.66, b = 87, c= 64.91 A and beta = 96.43. PGL in complex with citrate has unit cell parameters of a = 49.46, b = 87.21, c= 64.79 A and beta = 96.53
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hanging-drop method, equilibrated against a reservoir solution of 100 mM Hepes (pH 7.5), 30% (v/v) PEG MME 2000, 50 mM sodium acetate
hanging drop vapour diffusion method, using 0.1 M Bis-Tris pH 5.5, 25% (w/v) PEG 3350, and 0.2 M ammonium acetate
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