3.1.1.31: 6-phosphogluconolactonase
This is an abbreviated version!
For detailed information about 6-phosphogluconolactonase, go to the full flat file.
Reaction
Synonyms
6-PGL, 6-phospho-D-glucose-delta-lactone hydrolase, 6-phosphoglucono-gamma-lactonase, 6-phosphogluconolactonase, 6-phosphogluconolactonase 3, 6-phosphonogluconolactonase, 6PGL, 6PGL-G6PDH, G6PD-6PGL, GL50803_8682, Glc6PD-6PGL, glucose-6-phosphate dehydrogenase-6-phosphogluconolactonase, glucose-6-phosphate dehydrognease-6-phosphogluconolactonase, GluPho, lactonase, phosphoglucono-, OG1RF_11582, PF14_0511, Pf6PGL, PGL, PGL1, PGL2, PGL3, PGL4, PGL5, PglA, phosphogluconolactonase, Xoo2316, YbhE protein
ECTree
3.1.1.31 6-phosphogluconolactonaseAdvanced search results
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results (Cloned
Cloned on EC 3.1.1.31 - 6-phosphogluconolactonase
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CLONED (Commentary) 
ORGANISM
UNIPROT
LITERATURE
cloning of the gene encoding PfGluPho from chromosome 14 of the 3D7 gametocyte cDNA library, heterologous overexpression of PfGluPho using vector pQE30 in Escherichia coli strain M15
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expressed as a His fusion protein in Escherichia coli BL21(DE3) cells
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expression in Escherichia coli
expression of lactonase domain Pf6PGL in Escherichia coli, low recovery of soluble Pf6PGL
gene h6PGL, overexpression as N-terminally His-tagged enzyme in Escherichia coli strain BL21 using vector pRAREII
gene PGL3, presence of the C-terminal SKL motif interfered with chloroplast import of a PGL3_N-full-CFP fusion and with that of a PGL3_long medial-CFP fusion (with free N- and C-terminal ends) exclusively labeling peroxisomes, co-expression of thioredoxin Trxm2 with C-terminally truncated enzyme PGL3_N-short and _N-long versions accumulate in the cytosol of tobacco protoplasts and co-localization with Trxm2 in the cytosol and in the chloroplast stroma, recombinant expression of N-terminally CFP-tagged, green-fluorescent enzyme in Nicotiana benthamiana leaves
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PCR product cloned into the corresponding sites of the vector pGEX-4T-1 and then introduced into Escherichia coli. Constructs (35S:DELTANTpgl3-GFP and 35S:pgl3 DELTASKL-GFP) together with the 35S:PGL3-GFP construct transformed into the pgl3 background. Escherichia coli cells expressing GST-PGL3 and GST-pgl3 mutants
