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3.1.1.1: carboxylesterase

This is an abbreviated version!
For detailed information about carboxylesterase, go to the full flat file.

Word Map on EC 3.1.1.1

Reaction

a carboxylic ester
+
H2O
=
an alcohol
 +
a carboxylate

Synonyms

4-nitrophenyl esterase, ACAT, Acetyl esterase, acetyl xylan esterase, acid retinyl ester hydrolase, AcXE, Acyl coenzyme A:cholesterol acyltransferase, acylcarnitine hydrolase, AF1716, AF1763, AFEST, ali-esterase, aliesterase, alpha-carboxylesterase, alpha-esterase 7, alphaE7, alphaEsterase7, AOPP herbicide carboxylesterase, APE1547, AREH, aspirin hydrolase, Axe1NaM1, Axe1NaM2, Axe1NaM3, B-esterase, Bacillus esterase, BdB1, BioHe, BioHs, Brain carboxylesterase hBr1, BSE, BsE-NP01, BSE01701, butyrate esterase, butyryl esterase, CaE, CaesCCR11, carboxyesterase, Carboxyesterase ES-10, carboxyl ester hydrolase, carboxyl esterase, carboxyl/cholinesterase, carboxylate esterase, carboxylesterase, carboxylesterase 1, carboxylesterase 1A2, carboxylesterase 1C, carboxylesterase 2, carboxylesterase B, carboxylesterase B1, carboxylesterase-1, carboxylesterase-2, Carboxylesterase-5C, carboxylic acid esterase, carboxylic ester hydrolase, carboxylic esterase, Carboxylic-ester hydrolase, CarE, CbE, CCE, CE, CE-1, CE-2, CE1, CE2, CE21p, CE7 AcXE, CEH, CES, CES 1C, CES-2, CES1, CES1-b, CES1-c, CES1A, CES1A1, CES1A2, Ces1c, Ces1d, Ces1e, Ces1g, CES2, CES2A1, Ces3-1, Ces3a, cholesteryl ester hydrolase, cocaine esterase, cocaine:benzoylecgonine esterase, COE, CPT-CE, CXE, CXE1, CXE10, D1CarE5, de-arenethiolase, DEHP, E3 carboxylesterase, E34Tt, EC 3.1.1.21, Egasyn, ES-10, Es-22, ES-HTEL, ES-HVEL, ES-Male, ES-THET, ES10, ES11, ES3, ES4, ES5, ES6, ES7, ES9, EST, EST-3, EST-4, EST-5A, EST-5B, EST-5C, Est-AF, Est0071, Est1, Est1C, EST2, ESt3, Est3 esterase, Est30, Est55, EstA, EstA esterase, EstB, estBB1, EstC, EstC1, esterase, esterase 1F, esterase 2, esterase 2B, esterase 6A, esterase 9A, esterase A, esterase B, esterase B1, esterase D, esterase Sso2518, esterase, carboxyl, Esterase-22, Esterase-31, esterase-6, EStFa, EstGtA2, EstP, EstPS1, EstSt7, EstU1, Fluazifop-P-butyl carboxylesterase, Fluazifop-P-butyl hydrolase, FpbH, fungus-inducible pepper carboxylesterase, general odorant degradation enzyme, GODE, hCE-2, HCE1, HCE2, hCES1, hiCE, HMSE, hormone-sensitive lipase, HSL, human carboxylesterase 1, human carboxylesterase 2, human liver carboxylesterase, ICE, ICXE14, JHE-related, JHER, kidney bean esterase, Kidney microsomal carboxylesterase, LcalphaE7, LIP4, LipA, LipC, Liver microsomal carboxylesterase, malathion carboxylesterase, MAP esterase, MCE, ME, MeIAA esterase, MeSA esterase, methylbutyrase, methylbutyrate esterase, mfCES2v3, Microsomal palmitoyl-CoA hydrolase, monobutyrase, Monocyte/macrophage serine esterase, More, mouse carboxylesterase, MT2282, NaM1, NaM2, NaM3, Non-specific carboxylesterase, nonspecific carboxylesterase, ODE, odorant degradation enzyme, P186_1588, PA3859, PepEST, PF2001, Pf2001 esterase, PI 5.5 esterase, PI 6.1 esterase, pig liver esterase, PLE, PMPMEase, pnbA, PnbA1, PnbA2, polyisoprenylated methylated protein methyl esterase, porcine liver carboxylesterase, PPE, PPMTase, PrbA, prenylated methylated protein methyl esterase, procaine esterase, Proline-beta-naphthylamidase, propionyl esterase, pyrethroid-hydrolysing esterase, Rsp3690, SABP2's methyl salicylate esterase, salicylic acid-binding protein 2, SeE, serine protease-like, serine-type carboxylesterase, SexiCXE10, SiRe0290, SshEstI, SshEstI esterase, Sso P1 carboxylesterase, Sso-EST1, SSO2493, SSO2517, SSoP1 CE, SsoPEst, ST0071, ST2026, STK_00710, Sto-Est, TGH, triacetin esterase, triacylglycerol hydrolase, vitamin A esterase, VmoLac

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.1 carboxylesterase

Temperature Stability

Temperature Stability on EC 3.1.1.1 - carboxylesterase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0 - 40
stable for at least 16 h
126
-
half-life: 50 min
20
-
3 min, about 15% loss of activity
25 - 45
stable up to, activity drops sharply above 45°C
25 - 55
PnbA2 remains stable at temperatures ranging from 25-40°C, and stability of the enzyme decreases gradually above 40°C. PnbA2 activity gradually decreases with temperature increasing, the enzyme retain a part of its activity at 55°C
27 - 100
at 27°C, 70°C, and 100°C the enzyme is stable and maintains its global three-dimensional structure
30
60 min, more than 90% remaining activity
30 - 80
36
-
30 min, stable up to
38
-
19 days, 90% residual activity in phosphate buffer, pH 7.5, 78% residual activity in Tris/HCl buffer, pH 7.5
4
purified recombinant His-tagged enzyme, 12 h, loss of 40% activity
4 - 20
-
the enzyme, incubated at 20°C, shows a partial de-activation after 12 h, the enzyme incubated at 4°C displays a constant activity over a 3 days period
45 - 50
-
denaturation occurs
45 - 80
-
LIP4 is stable at temperatures below 45°C and loses about 36% of its activity when incubated for 60 min at 50°C, while the enzyme is completely inactivated at 80°C. The half-life of the enzyme is 103 min at 50°C, 36 min at 55°C, and 9 min at 60°C without any additive
47 - 70
-
the wild-type enzyme has a melting temperature (Tm) of 47°C and an unfolding transition at 45°C, while the double mutant NaM1H2 melts at 63°C and unfolds at 60°C. The mutants NaM1N96S and NaM1F210L show thermal unfolding at 50°C and have a melting temperature of 52°C, and lose all secondary structures at 70°C
50 - 60
the reaction rates dramatically slow down with time at 50 and 60°C
60 - 100
enzyme EstPS1 shows high thermostability, and the half-lives at 60, 70, 80, 90, and 100°C are 14 h, 2 h, 31 min, 10 min, and 1 min, respectively
60 - 75
-
at 60 and 65°C, the enzyme is stable for half an hour; whereas incubation at 70°C for half an h leads to a 50% loss of activity. At 65°C, the stability of carboxylesterase decreases to 70% of the total. After 70°C, a sharp decline in the enzyme activity is observed
60 - 80
the enzyme is stable for 42 h at 60°C, however an important decrease in stability is observed at 70°C, where the enzyme retains 63% of its initial activity, down to 43% at 75°C and further to 75% at 80°C after 10 min
65 - 90
-
more than 90% activity remains at 65°C or 70°C, 10% of activity remains after 3 h incubation at 90°C using ketoprofen ethyl ester as substrate
70 - 160
-
bovine serum albumin exhibits a 133fold increase in its turnover number toward 4-nitrophenyl palmitate from 70 to 150°C, at 160°C a slight decrease in esterase activity is observed
70 - 74
-
isozymr Ces3-1 retains maximum catalytic activity when incubated for 15 min up to 70°C, after which the esterase is rapidly inactivated displaying no activity after treatment at 74°C for 15 min
71
-
50% inactivation after 1 h
72
Tm-value at pH 4.0, wild-type enzyme
76
Tm-value at pH 6.0 and 7.0, mutant enzyme R267E
81
Tm-value at pH 6.0, wild-type enzyme
83
Tm-value at pH 5.0, wild-type enzyme
86
Tm-value at pH 9.0, wild-type enzyme
87
Tm-value at pH 7.0, wild-type enzyme
additional information