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3.1.1.1: carboxylesterase

This is an abbreviated version!
For detailed information about carboxylesterase, go to the full flat file.

Word Map on EC 3.1.1.1

Reaction

a carboxylic ester
+
H2O
=
an alcohol
 +
a carboxylate

Synonyms

4-nitrophenyl esterase, ACAT, Acetyl esterase, acetyl xylan esterase, acid retinyl ester hydrolase, AcXE, Acyl coenzyme A:cholesterol acyltransferase, acylcarnitine hydrolase, AF1716, AF1763, AFEST, ali-esterase, aliesterase, alpha-carboxylesterase, alpha-esterase 7, alphaE7, alphaEsterase7, AOPP herbicide carboxylesterase, APE1547, AREH, aspirin hydrolase, Axe1NaM1, Axe1NaM2, Axe1NaM3, B-esterase, Bacillus esterase, BdB1, BioHe, BioHs, Brain carboxylesterase hBr1, BSE, BsE-NP01, BSE01701, butyrate esterase, butyryl esterase, CaE, CaesCCR11, carboxyesterase, Carboxyesterase ES-10, carboxyl ester hydrolase, carboxyl esterase, carboxyl/cholinesterase, carboxylate esterase, carboxylesterase, carboxylesterase 1, carboxylesterase 1A2, carboxylesterase 1C, carboxylesterase 2, carboxylesterase B, carboxylesterase B1, carboxylesterase-1, carboxylesterase-2, Carboxylesterase-5C, carboxylic acid esterase, carboxylic ester hydrolase, carboxylic esterase, Carboxylic-ester hydrolase, CarE, CbE, CCE, CE, CE-1, CE-2, CE1, CE2, CE21p, CE7 AcXE, CEH, CES, CES 1C, CES-2, CES1, CES1-b, CES1-c, CES1A, CES1A1, CES1A2, Ces1c, Ces1d, Ces1e, Ces1g, CES2, CES2A1, Ces3-1, Ces3a, cholesteryl ester hydrolase, cocaine esterase, cocaine:benzoylecgonine esterase, COE, CPT-CE, CXE, CXE1, CXE10, D1CarE5, de-arenethiolase, DEHP, E3 carboxylesterase, E34Tt, EC 3.1.1.21, Egasyn, ES-10, Es-22, ES-HTEL, ES-HVEL, ES-Male, ES-THET, ES10, ES11, ES3, ES4, ES5, ES6, ES7, ES9, EST, EST-3, EST-4, EST-5A, EST-5B, EST-5C, Est-AF, Est0071, Est1, Est1C, EST2, ESt3, Est3 esterase, Est30, Est55, EstA, EstA esterase, EstB, estBB1, EstC, EstC1, esterase, esterase 1F, esterase 2, esterase 2B, esterase 6A, esterase 9A, esterase A, esterase B, esterase B1, esterase D, esterase Sso2518, esterase, carboxyl, Esterase-22, Esterase-31, esterase-6, EStFa, EstGtA2, EstP, EstPS1, EstSt7, EstU1, Fluazifop-P-butyl carboxylesterase, Fluazifop-P-butyl hydrolase, FpbH, fungus-inducible pepper carboxylesterase, general odorant degradation enzyme, GODE, hCE-2, HCE1, HCE2, hCES1, hiCE, HMSE, hormone-sensitive lipase, HSL, human carboxylesterase 1, human carboxylesterase 2, human liver carboxylesterase, ICE, ICXE14, JHE-related, JHER, kidney bean esterase, Kidney microsomal carboxylesterase, LcalphaE7, LIP4, LipA, LipC, Liver microsomal carboxylesterase, malathion carboxylesterase, MAP esterase, MCE, ME, MeIAA esterase, MeSA esterase, methylbutyrase, methylbutyrate esterase, mfCES2v3, Microsomal palmitoyl-CoA hydrolase, monobutyrase, Monocyte/macrophage serine esterase, More, mouse carboxylesterase, MT2282, NaM1, NaM2, NaM3, Non-specific carboxylesterase, nonspecific carboxylesterase, ODE, odorant degradation enzyme, P186_1588, PA3859, PepEST, PF2001, Pf2001 esterase, PI 5.5 esterase, PI 6.1 esterase, pig liver esterase, PLE, PMPMEase, pnbA, PnbA1, PnbA2, polyisoprenylated methylated protein methyl esterase, porcine liver carboxylesterase, PPE, PPMTase, PrbA, prenylated methylated protein methyl esterase, procaine esterase, Proline-beta-naphthylamidase, propionyl esterase, pyrethroid-hydrolysing esterase, Rsp3690, SABP2's methyl salicylate esterase, salicylic acid-binding protein 2, SeE, serine protease-like, serine-type carboxylesterase, SexiCXE10, SiRe0290, SshEstI, SshEstI esterase, Sso P1 carboxylesterase, Sso-EST1, SSO2493, SSO2517, SSoP1 CE, SsoPEst, ST0071, ST2026, STK_00710, Sto-Est, TGH, triacetin esterase, triacylglycerol hydrolase, vitamin A esterase, VmoLac

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.1 carboxylesterase

Specific Activity

Specific Activity on EC 3.1.1.1 - carboxylesterase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0000544
2alpha,17alpha-diethynyl-A-nor-5alpha-androstane-2beta,17beta-diol dipropionate hydrolysis, intestinal microsomes, pH 7.4, 37°C
0.000123
2alpha,17alpha-diethynyl-A-nor-5alpha-androstane-2beta,17beta-diol dipropionate hydrolysis, CES1, pH 7.4, 37°C
0.000137
2alpha,17alpha-diethynyl-A-nor-5alpha-androstane-2beta,17beta-diol dipropionate hydrolysis, CES2, pH 7.4, 37°C
0.00023
-
purified enzyme, substrate 4-nitrophenyl acetate
0.0004
-
crude extract
0.000476
2alpha,17alpha-diethynyl-A-nor-5alpha-androstane-2beta,17beta-diol dipropionate hydrolysis, liver microsomes, pH 7.4, 37°C
0.0009
-
purified enzyme, substrate butyl paraben
0.00094
substrate (2S)-fenvalerate, pH 8.0, 30°C
0.00097
substrate cocaine, pH 7.4, temperature not specified in the publication
0.0014
substrate (alphaR)(2S)-fenvalerate, pH 8.0, 30°C
0.0019
-
purified enzyme, substrate propyl paraben
0.0025
-
purified enzyme, substrate methyl paraben
0.0027
-
purified enzyme, substrate ethyl paraben
0.00334
procaine hydrolysis, CES2 isozyme, pH 7.4, 37°C
0.004
procaine hydrolysis, liver microsomes, pH 7.4, 37°C
0.00529
-
after 13.23fold purification
0.0072
substrate (alphaS)(2R)-fenvalerate, pH 8.0, 30°C
0.01
-
isoform CesB, substrate beta-naphthylacetate, pH 7.5, 32°C
0.02
substrate cyano(6-methoxynaphthalen-2-yl)methyl (2S)-2-(4-chlorophenyl)-3-methylbutanoic acid, pH 8.0, 30°C
0.027
substrate cis-permethrin, pH 8.0, 30°C
0.029
0.03
-
isoform Cesb, substrate (R)-naproxen methyl ester, pH 7.5, 32°C
0.045
substrate alpha-cypermethrin, pH 8.0, 30°C
0.06
substrate cis-cypermethrin, pH 8.0, 30°C
0.061
substrate cyano(6-methoxy-2-naphthyl)methyl 3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropanecarboxylate, pH 8.0, 30°C
0.075
substrate (alphaR)(2R)-fenvalerate, pH 8.0, 30°C
0.08
-
isoform CesA, substrate beta-naphthylacetate, pH 7.5, 32°C
0.0928
fenofibrate hydrolysis, CES1 isozyme, pH 7.4, 37°C
0.095
substrate epsilon-cypermethrin, pH 8.0, 30°C
0.099
-
isoform CES2, using methyl 4-nitrobenzoate as substrate, in 0.1 M potassium phosphate buffer, pH 7.4, at 37°C
0.1
-
substrate beta-naphthylacetate, pH 7.5, 32°C
0.113
-
crude extract, at 25°C, pH 7.2
0.124
-
purified malathion carboxylesterase, substrate malathion
0.17
-
isoform CesB, substrate (S)-naproxen methyl ester, pH 7.5, 32°C
0.19
+/- 0.02, palmitoyl-CoA
0.213
fenofibrate hydrolysis, liver microsomes, pH 7.4, 37°C
0.239
substrate permethrin, pH 8.0, 30°C
0.246
substrate trans-cypermethrin, pH 8.0, 30°C
0.281
-
isoform CES1-c, fluorescein diacetate as substrate, in 0.1 M potassium phosphate buffer, pH 7.4, at 37°C
0.29
-
purified isozyme ME3, substrate alpha-naphthyl acetate
0.35
-
purified isozyme ME2, substrate trans-permethrin
0.357
-
isoform CES1-b, using fluorescein diacetate as substrate, in 0.1 M potassium phosphate buffer, pH 7.4, at 37°C
0.36
-
purified isozyme ME1, substrate trans-permethrin
0.53
substrate p-nitrophenyl deltamethrin, pH 7.0, 30°C
0.55
-
isoform CesB, substrate Rac-ibuprofen methyl ester, pH 7.5, 32°C
0.597
substrate trans-permethrin, pH 8.0, 30°C
0.65
-
purified isozyme ME3, substrate trans-permethrin
0.68
-
isoform CES1-c, methyl 4-nitrobenzoate as substrate, in 0.1 M potassium phosphate buffer, pH 7.4, at 37°C
0.682
-
isoform CES2, using 4-nitrophenyl acetate as substrate, in 0.1 M potassium phosphate buffer, pH 7.4, at 37°C
0.76
-
purified isozyme ME1, substrate alpha-naphthyl acetate
0.77
-
crude extract from strain VOSCM, using 4-nitrophenyl acetate as substrate, in 9.2 mM Tris-HCl buffer, pH 7.5, at 25°C
0.84
-
purified isozyme ME2, substrate alpha-naphthyl acetate
0.843
-
purified native enzyme, pH 6.5, 35°C, substrate 2-naphthyl acetate
0.957
-
isoform CES1-b, using methyl 4-nitrobenzoate as substrate, in 0.1 M potassium phosphate buffer, pH 7.4, at 37°C
1
-
substrate p-nitrophenyl octanoate, 45°C, pH 7.0
1.4
-
isoform CES1-c, using 4-nitrophenyl acetate as substrate, in 0.1 M potassium phosphate buffer, pH 7.4, at 37°C
1.43
-
isoform CES1-b, using 4-nitrophenyl acetate as substrate, in 0.1 M potassium phosphate buffer, pH 7.4, at 37°C
1.86
-
purified recombinant mutant NaM1H2, pH 8.5, 30°C, substrate 4-nitrophenyl octanoate
102
phenacetin hydrolysis, intestinal microsomes, pH 7.4, 37°C
106.25
-
purified recombinant mutant NaM1H2, pH 8.5, 30°C, substrate 7-aminocephalosporanic acid
107.5
-
substrate vinyl acetate, 45°C, pH 7.0
109
purified recombinant refolded His-taggged mutant A270S, pH 7.5, 37°C, substrate 4-nitrophenyl acetate
1095
-
crude extract, using alpha-naphthyl acetate as substrate, at 25°C
11.78
-
using 4-nitrophenyl hexanoate as substrate, at 35°C in 100 mM sodium phosphate (pH 7.0)
1123
-
substrate vinyl butanoate, pH 7.0, 25°C
113.2
-
isoform hCE1, substrate 4-nitrophenyl valerate, pH 7.4, 37°C
1160
pH 7.1, 70°C, substrate: butyl acetate
12.7
+/- 1.5, p-nitrophenyl butyrate
12.96
-
purified recombinant wild-type enzyme, pH 8.5, 30°C, substrate 4-nitrophenyl butyrate
120
-
pH 7.0, 37°C, isoform CES2
126
-
substrate 4-nitrophenyl valerate, pH 7.4, 37°C
128
purified recombinant refolded His-taggged mutant S76N, pH 7.5, 37°C, substrate 4-nitrophenyl acetate
13
substrate: 4-nitrophenyl hexadecanoate, pH 9.0, 80°C
13.6
purified recombinant wild-type enzyme, substrate carbaryl, pH 8.0, 60°C
13.8
-
isoform CES2, using fluorescein diacetate as substrate, in 0.1 M potassium phosphate buffer, pH 7.4, at 37°C
135
-
purified enzyme, substrate ethyl benzoate
1350
-
substrate tripropionin, pH 7.0, 25°C
1375.7
purified recombinant enzyme, pH 6.5, 30°C, substrate 4-nitrophenyl laurate
1392
pH 8.0, 60°C, purified enzyme
1440
-
purified enzyme
147.5
-
substrate triacetin, 45°C, pH 7.0
149
using tripropionin as substrate, at 25°C
15.9
-
-
1510
pH 7.1, 70°C, substrate: isobutyl acetate
16.7
purified isozyme ES4, substrate 4-methylumbelliferyl acetate
160
pH 7.1, 70°C, substrate: methyl acetate
160.1
-
-
1618
-
substrate vinyl propionate, pH 7.0, 25°C
162.2
-
purified recombinant mutant F210L, pH 8.5, 30°C, substrate 4-nitrophenyl acetate
167.5
-
substrate tributyrin, 45°C, pH 7.0
17
substrate 4-nitrophenyl acetate, pH 8.0, 30°C
1735
pH 7.5, 22°C
1750
-
pH 7.2, substrate 4-methylumbelliferyl octanoate
184
-
substrate triacetin, pH 7.0, 25°C
189
substrate: 4-nitrophenyl octanoate, pH 9.0, 80°C
19
substrate: 4-nitrophenyl tetradecanoate, pH 9.0, 80°C
19.05
-
isoform hCE2, substrate 4-nitrophenyl acetate, pH 7.4, 37°C
192
-
crude extract, using 4-nitrophenyl acetate as substrate, at 25°C
193
purified recombinant refolded His-taggged mutant D204E, pH 7.5, 37°C, substrate 4-nitrophenyl acetate
2 - 2.5
-
enzyme from strain VOSCM after 29.2fold purification, using 4-nitrophenyl acetate as substrate, in 9.2 mM Tris-HCl buffer, pH 7.5, at 25°C
2.4
substrate p-nitrophenyl malathion, pH 7.0, 30°C
2.58
using 4-nitrophenyl caprylate as substrate, at 50°C and pH 8.0
20.46
-
purified recombinant mutant NaM1H2, pH 8.5, 30°C, substrate 4-nitrophenyl butyrate
20.9
-
substrate Rac-ibuprofen methyl ester, pH 7.5, 32°C
200
-
purified recombinant wild-type enzyme, pH 8.5, 30°C, substrate 7-aminocephalosporanic acid
2046
-
after 8.11fold purification, using 4-nitrophenyl butyrate as substrate, at pH 8.5 and 40°C
211.7
-
purified recombinant mutant NaM1H2, pH 8.5, 30°C, substrate 4-methylumbelliferyl acetate
213
substrate: 4-nitrophenyl acetate, pH 9.0, 80°C
22.24
-
using 4-nitrophenyl butyrate as substrate, at 35°C in 100 mM sodium phosphate (pH 7.0)
22.9
-
substrate p-nitrophenyl valerate, 45°C, pH 7.0
222.2
-
purified recombinant wild-type enzyme, pH 8.5, 30°C, substrate 2-naphthyl acetate
2226
purified recombinant His-tagged wild-type enzyme, substrate 4-nitrophenol butyrate, pH 8.0, 60°C
24
using vinyl butyrate as substrate, at 25°C
246.6
substrate: 4-nitrophenyl acetate, pH 7.0, 30°C
25.4
-
isoform CesA, substrate Rac-ibuprofen methyl ester, pH 7.5, 32°C
252
-
crude extract, using 4-nitrophenyl butyrate as substrate, at pH 8.5 and 40°C
2598
purified recombinant enzyme, substrate 4-nitrophenyl butyrate
26
-
purified enzyme
26.7
-
substrate triacetin, 45°C, pH 7.0
269
substrate p-nitrophenyl propionate, pH 7.0, 30°C
27.5
+/- 2.5, p-nitrophenyl propionate
27000
purified recombinant enzyme, substrate (S)-ketoprofen ethyl ester
272
pH 7.1, 70°C, substrate: tert-butyl acetate
277.8
-
purified recombinant wild-type enzyme, pH 8.5, 30°C, substrate 4-methylumbelliferyl acetate
29.5
-
pH 7.0, 75°C
29.7
purified isozyme D50580, substrate 4-methylumbelliferyl acetate
293.1
-
isoform hCE1, substrate 4-nitrophenyl butanoate, pH 7.4, 37°C
3
using tributyrin as substrate, at 25°C
3.1
-
hydrolysis of palmitoyl-CoA
3.17
+/- 0.34, p-nitrophenyl laurate
3.5
substrate p-nitrophenyl fenvalerate, pH 7.0, 30°C
3.6
-
substrate p-nitrophenyl octanoate, 45°C, pH 7.0
3.75
-
using 4-nitrophenyl decanoate as substrate, at 35°C in 100 mM sodium phosphate (pH 7.0)
30.1
purified isozyme ES3, substrate 4-methylumbelliferyl acetate
30.9
-
substrate p-nitrophenyl decanoate, 45°C, pH 7.0
3000
80°C, pH 7.1, substrate: 4-nitrophenyl hexanoate
31.1
-
substrate p-nitrophenyl butanoate, 45°C, pH 7.0
313
pH 7.1, 70°C, substrate: ethyl acetate
315.2
-
isoform hCE2, substrate 4-nitrophenyl butanoate, pH 7.4, 37°C
317.2
65°C, pH 8.0
33.3
-
substrate tripropionin, 45°C, pH 7.0
348.3
-
purified recombinant mutant NaM1H2, pH 8.5, 30°C, substrate 4-nitrophenyl acetate
35.6
substrate p-nitrophenyl cis-permethrin, pH 7.0, 30°C
354
substrate: 4-nitrophenyl butyrate, pH 9.0, 80°C
3542
pH 9.0, 80°C, substrate: 4-nitrophenyl butyrate
355
substrate p-nitrophenyl butyrate, pH 7.0, 30°C
36.14
-
isoform hCE1, substrate 4-nitrophenyl acetate, pH 7.4, 37°C
3640
-
pH 7.2, substrate 4-methylumbelliferyl propionate
374
-
substrate 4-nitrophenyl butanoate, pH 7.4, 37°C
3751
-
pH 7.2, substrate 4-methylumbelliferyl butanoate
38.8
substrate p-nitrophenyl trans-permethrin, pH 7.0, 30°C
38.9
+/- 3.6, p-nitrophenyl acetate
380
-
substrate vinyl propionate, 45°C, pH 7.0
387
purified recombinant enzyme, substrate 4-nitrophenyl caprylate
387.5
390
pH 7.1, 70°C, substrate: isopropyl acetate
394.8
-
-
3967
-
pH 7.2, substrate 4-methylumbelliferyl acetate
3975
-
pH 7.0, 50°C
4
-
6 somite stage, 22°C
4.23
-
isoform CesA, substrate (S)-naproxen methyl ester, pH 7.5, 32°C
4.6
-
substrate (S)-naproxen methyl ester, pH 7.5, 32°C
40 - 140
-
isozyme CE-2
4050
pH 7.1, 70°C, substrate: 4-nitrophenyl hexanoate
407
substrate p-nitrophenyl caprylate, pH 7.0, 30°C
41.9
purified isozyme ES10, substrate 4-methylumbelliferyl acetate
425.7
429.5
-
-
44
using acetylsalicyclic acid as substrate, at 25°C
4534
-
pH 7.2, substrate 4-methylumbelliferyl 3-phenoxybutanoate
456
-
substrate tributyrine, pH 7.0, 25°C
48
purified renatured, recombinant enzyme, substrate p-tolyl octanoate
48.8
purified recombinant enzyme, in absence of NaCl
488.9 - 832.4
-
purified recombinant wild-type enzyme, pH 8.5, 30°C, substrate 4-nitrophenyl acetate
5.62
-
after 49.73fold purification, at 25°C, pH 7.2
5.8
-
pH 7.0, 37°C
50.4
phenacetin hydrolysis, liver microsomes, pH 7.4, 37°C
51
-
purified recombinant enzyme, 12.5% NaCl
513
using triacetin as substrate, at 25°C
513.6
-
substrate p-nitrophenyl butanoate, 45°C, pH 7.0
5242
purified recombinant enzyme, substrate 4-nitrophenyl acetate
54.7
-
substrate tributyrin, 45°C, pH 7.0
559
pH 7.1, 70°C, substrate: octyl acetate
56
substrate: 4-nitrophenyl decanoate, pH 9.0, 80°C
5634
-
pH 7.2, substrate 4-methylumbelliferyl pentanoate
573
substrate p-nitrophenyl caproate, pH 7.0, 30°C
5734
-
pH 7.2, substrate 4-methylumbelliferyl cyclopentane carboxylate
58.2
purified renatured, recombinant enzyme, substrate 4-nitrophenyl butyrate
590
pH 8.5, temperature not specified in the publication, substrate: vinyl butyrate
6.05
-
purified recombinant wild-type enzyme, pH 8.5, 30°C, substrate xylan
6.24
-
using 4-nitrophenyl acetate as substrate, at 35°C in 100 mM sodium phosphate (pH 7.0)
6.6
-
pH 7.0, 37°C, isoform CES1A1
600
-
recombinant enzyme
62
purified renatured, recombinant enzyme, substrate vinyl octanoate
66.7
-
hydrolysis of 4-nitrophenyl acetate
67
substrate p-nitrophenyl laurate, pH 7.0, 30°C
68
using ethyl acetate as substrate, at 25°C
7.4
-
substrate p-nitrophenyl caproate, 45°C, pH 7.0
7.46
-
using 4-nitrophenyl octanoate as substrate, at 35°C in 100 mM sodium phosphate (pH 7.0)
73
purified recombinant refolded His-taggged wild-type enzyme, pH 7.5, 37°C, substrate 4-nitrophenyl acetate
747
pH 7.1, 70°C, substrate: hexyl acetate
750
-
pH 7.2, substrate 4-methylumbelliferyl (2E)-but-2-enoate
77
purified recombinant enzyme, substrate 4-nitrophenyl palmitate
78.51
-
purified recombinant mutant NaM1H2, pH 8.5, 30°C, substrate 2-naphthyl acetate
7902
-
pH 7.2, substrate 4-methylumbelliferyl hexanoate
8.4
+/- 0.27, p-nitrophenyl caproate
805
pH 7.1, 70°C, substrate: propyl acetate
815
using vinyl propionate as substrate, at 25°C
831
-
pH 6.8, 37°C
86
purified renatured, recombinant enzyme, substrate vinyl hexanoate
86.41
-
substrate 4-nitrophenyl acetate, pH 7.4, 37°C
876
pH 7.1, 70°C, substrate: sec-butyl acetate
88.2
-
purified enzyme
9.8
substrate p-nitrophenyl cypermethrin, pH 7.0, 30°C
90.1
-
isoform hCE2, substrate 4-nitrophenyl valerate, pH 7.4, 37°C
91
substrate p-nitrophenyl acetate, pH 7.0, 30°C
92
-
pH 7.5
955.4
-
purified recombinant mutant N96S, pH 8.5, 30°C, substrate 4-nitrophenyl acetate
973
purified recombinant His-tagged AT-domain truncated enzyme mutant, substrate 4-nitrophenol butyrate, pH 8.0, 60°C
additional information