Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.1.1.1: carboxylesterase

This is an abbreviated version!
For detailed information about carboxylesterase, go to the full flat file.

Word Map on EC 3.1.1.1

Reaction

a carboxylic ester
+
H2O
=
an alcohol
+
a carboxylate

Synonyms

4-nitrophenyl esterase, ACAT, Acetyl esterase, acetyl xylan esterase, acid retinyl ester hydrolase, AcXE, Acyl coenzyme A:cholesterol acyltransferase, acylcarnitine hydrolase, AF1716, AF1763, AFEST, ali-esterase, aliesterase, alpha-carboxylesterase, alpha-esterase 7, alphaE7, alphaEsterase7, AOPP herbicide carboxylesterase, APE1547, AREH, aspirin hydrolase, Axe1NaM1, Axe1NaM2, Axe1NaM3, B-esterase, Bacillus esterase, BdB1, BioHe, BioHs, Brain carboxylesterase hBr1, BSE, BsE-NP01, BSE01701, butyrate esterase, butyryl esterase, CaE, CaesCCR11, carboxyesterase, Carboxyesterase ES-10, carboxyl ester hydrolase, carboxyl esterase, carboxyl/cholinesterase, carboxylate esterase, carboxylesterase, carboxylesterase 1, carboxylesterase 1A2, carboxylesterase 1C, carboxylesterase 2, carboxylesterase B, carboxylesterase B1, carboxylesterase-1, carboxylesterase-2, Carboxylesterase-5C, carboxylic acid esterase, carboxylic ester hydrolase, carboxylic esterase, Carboxylic-ester hydrolase, CarE, CbE, CCE, CE, CE-1, CE-2, CE1, CE2, CE21p, CE7 AcXE, CEH, CES, CES 1C, CES-2, CES1, CES1-b, CES1-c, CES1A, CES1A1, CES1A2, Ces1c, Ces1d, Ces1e, Ces1g, CES2, CES2A1, Ces3-1, Ces3a, cholesteryl ester hydrolase, cocaine esterase, cocaine:benzoylecgonine esterase, COE, CPT-CE, CXE, CXE1, CXE10, D1CarE5, de-arenethiolase, DEHP, E3 carboxylesterase, E34Tt, EC 3.1.1.21, Egasyn, ES-10, Es-22, ES-HTEL, ES-HVEL, ES-Male, ES-THET, ES10, ES11, ES3, ES4, ES5, ES6, ES7, ES9, EST, EST-3, EST-4, EST-5A, EST-5B, EST-5C, Est-AF, Est0071, Est1, Est1C, EST2, ESt3, Est3 esterase, Est30, Est55, EstA, EstA esterase, EstB, estBB1, EstC, EstC1, esterase, esterase 1F, esterase 2, esterase 2B, esterase 6A, esterase 9A, esterase A, esterase B, esterase B1, esterase D, esterase Sso2518, esterase, carboxyl, Esterase-22, Esterase-31, esterase-6, EStFa, EstGtA2, EstP, EstPS1, EstSt7, EstU1, Fluazifop-P-butyl carboxylesterase, Fluazifop-P-butyl hydrolase, FpbH, fungus-inducible pepper carboxylesterase, general odorant degradation enzyme, GODE, hCE-2, HCE1, HCE2, hCES1, hiCE, HMSE, hormone-sensitive lipase, HSL, human carboxylesterase 1, human carboxylesterase 2, human liver carboxylesterase, ICE, ICXE14, JHE-related, JHER, kidney bean esterase, Kidney microsomal carboxylesterase, LcalphaE7, LIP4, LipA, LipC, Liver microsomal carboxylesterase, malathion carboxylesterase, MAP esterase, MCE, ME, MeIAA esterase, MeSA esterase, methylbutyrase, methylbutyrate esterase, mfCES2v3, Microsomal palmitoyl-CoA hydrolase, monobutyrase, Monocyte/macrophage serine esterase, More, mouse carboxylesterase, MT2282, NaM1, NaM2, NaM3, Non-specific carboxylesterase, nonspecific carboxylesterase, ODE, odorant degradation enzyme, P186_1588, PA3859, PepEST, PF2001, Pf2001 esterase, PI 5.5 esterase, PI 6.1 esterase, pig liver esterase, PLE, PMPMEase, pnbA, PnbA1, PnbA2, polyisoprenylated methylated protein methyl esterase, porcine liver carboxylesterase, PPE, PPMTase, PrbA, prenylated methylated protein methyl esterase, procaine esterase, Proline-beta-naphthylamidase, propionyl esterase, pyrethroid-hydrolysing esterase, Rsp3690, SABP2's methyl salicylate esterase, salicylic acid-binding protein 2, SeE, serine protease-like, serine-type carboxylesterase, SexiCXE10, SiRe0290, SshEstI, SshEstI esterase, Sso P1 carboxylesterase, Sso-EST1, SSO2493, SSO2517, SSoP1 CE, SsoPEst, ST0071, ST2026, STK_00710, Sto-Est, TGH, triacetin esterase, triacylglycerol hydrolase, vitamin A esterase, VmoLac

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.1 carboxylesterase

Organic Solvent Stability

Organic Solvent Stability on EC 3.1.1.1 - carboxylesterase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1,4-dioxane
1-butanol
30% v/v, recombinant enzyme expressed in Sulfolobus solfataricus shows 36% residual activity. The recombinant enzyme expressed in Escherichia coli as thioredoxin-free form (EcSisEstA I) shows 18% residual activity. The recombinant enzyme expressed in Escherichia coli as a thioredoxin-EstA fusion protein (EcSisEstA II) shows 29% residual activity
1-propanol
30% v/v, recombinant enzyme expressed in Sulfolobus solfataricus shows 76% residual activity. The recombinant enzyme expressed in Escherichia coli as thioredoxin-free form (EcSisEstA I) shows 32% residual activity. The recombinant enzyme expressed in Escherichia coli as a thioredoxin-EstA fusion protein (EcSisEstA II) shows 39% residual activity
2-propanol
Acetone
acetonitrile
benzene
-
pH 7.0, 60°C, 2 h, loss of 91.6% activity
Butanol
chloroform
cyclohexane
-
pH 7.0, 60°C, 2 h, loss of 98.7% activity
dichloromethane
the enzyme activity is reduced by 73-91% at 5-10% v/v
diethyl ether
-
30%, 124% residual activity
dimethyl formamide
dimethyl sulfoxide
dimethylformamide
dimethylsulfoxide
dioxane
dodecane
-
5%, complete loss of activity
Ethanol
Ethyl acetate
formaldehyde
guanidine-HCl
half-completion of the unfolding transition: 2.0-2.1 M
heptane
hexadecane
-
5%, 51% residual activity
hexane
isoamyl alcohol
30% v/v, recombinant enzyme expressed in Sulfolobus solfataricus shows 36% residual activity. The recombinant enzyme expressed in Escherichia coli as thioredoxin-free form (EcSisEstA I) shows 8% residual activity. The recombinant enzyme expressed in Escherichia coli as a thioredoxin-EstA fusion protein (EcSisEstA II) shows 9% residual activity
isooctane
-
pH 7.0, 60°C, 2 h, completely stable
isopropanol
isopropyl ether
-
15% (v/v) isopropyl ether increases the esterase activity by about 60%, 50% (v/v) isopropyl ether produces a 10% increase in esterase activity
Methanol
N,N-dimethylformamide
-
30%, 108% residual activity
n-decane
the enzyme activity is reduced by 11-23% at 5-10% v/v
n-heptane
the enzyme activity is stable at 5-10% v/v
n-hexane
n-octanol
the enzyme activity is reduced by 22-90% at 5-10% v/v
n-propanol
the enzyme activity is reduced by 93.5% at 10% v/v
octane
-
5%, complete loss of activity
propanol
Spa-80
-
decreases activity at 0.1% (v/v) and 1% (v/v)
taurocholic acid
-
slightly increases activity at 0.1% (v/v)
tert-butyl alcohol
tetrahydrofuran
the enzyme activity is reduced by 33-96% at 5-10% v/v
toluene
Triton X-100
Triton X-15
-
110% increase of activity in the presence of Triton X-15
Triton X-165
-
1649% increase of activity in the presence of Triton X-15
Triton X-305
-
1528% increase of activity in the presence of Triton X-15
Triton X-45
-
562% increase of activity in the presence of Triton X-15
Tween
xylene
the enzyme activity is reduced by 44% at 5-10% v/v
additional information
-
investigation of stability of the enzyme in organic solvents