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3.1.1.1: carboxylesterase

This is an abbreviated version!
For detailed information about carboxylesterase, go to the full flat file.

Word Map on EC 3.1.1.1

Reaction

a carboxylic ester
+
H2O
=
an alcohol
 +
a carboxylate

Synonyms

4-nitrophenyl esterase, ACAT, Acetyl esterase, acetyl xylan esterase, acid retinyl ester hydrolase, AcXE, Acyl coenzyme A:cholesterol acyltransferase, acylcarnitine hydrolase, AF1716, AF1763, AFEST, ali-esterase, aliesterase, alpha-carboxylesterase, alpha-esterase 7, alphaE7, alphaEsterase7, AOPP herbicide carboxylesterase, APE1547, AREH, aspirin hydrolase, Axe1NaM1, Axe1NaM2, Axe1NaM3, B-esterase, Bacillus esterase, BdB1, BioHe, BioHs, Brain carboxylesterase hBr1, BSE, BsE-NP01, BSE01701, butyrate esterase, butyryl esterase, CaE, CaesCCR11, carboxyesterase, Carboxyesterase ES-10, carboxyl ester hydrolase, carboxyl esterase, carboxyl/cholinesterase, carboxylate esterase, carboxylesterase, carboxylesterase 1, carboxylesterase 1A2, carboxylesterase 1C, carboxylesterase 2, carboxylesterase B, carboxylesterase B1, carboxylesterase-1, carboxylesterase-2, Carboxylesterase-5C, carboxylic acid esterase, carboxylic ester hydrolase, carboxylic esterase, Carboxylic-ester hydrolase, CarE, CbE, CCE, CE, CE-1, CE-2, CE1, CE2, CE21p, CE7 AcXE, CEH, CES, CES 1C, CES-2, CES1, CES1-b, CES1-c, CES1A, CES1A1, CES1A2, Ces1c, Ces1d, Ces1e, Ces1g, CES2, CES2A1, Ces3-1, Ces3a, cholesteryl ester hydrolase, cocaine esterase, cocaine:benzoylecgonine esterase, COE, CPT-CE, CXE, CXE1, CXE10, D1CarE5, de-arenethiolase, DEHP, E3 carboxylesterase, E34Tt, EC 3.1.1.21, Egasyn, ES-10, Es-22, ES-HTEL, ES-HVEL, ES-Male, ES-THET, ES10, ES11, ES3, ES4, ES5, ES6, ES7, ES9, EST, EST-3, EST-4, EST-5A, EST-5B, EST-5C, Est-AF, Est0071, Est1, Est1C, EST2, ESt3, Est3 esterase, Est30, Est55, EstA, EstA esterase, EstB, estBB1, EstC, EstC1, esterase, esterase 1F, esterase 2, esterase 2B, esterase 6A, esterase 9A, esterase A, esterase B, esterase B1, esterase D, esterase Sso2518, esterase, carboxyl, Esterase-22, Esterase-31, esterase-6, EStFa, EstGtA2, EstP, EstPS1, EstSt7, EstU1, Fluazifop-P-butyl carboxylesterase, Fluazifop-P-butyl hydrolase, FpbH, fungus-inducible pepper carboxylesterase, general odorant degradation enzyme, GODE, hCE-2, HCE1, HCE2, hCES1, hiCE, HMSE, hormone-sensitive lipase, HSL, human carboxylesterase 1, human carboxylesterase 2, human liver carboxylesterase, ICE, ICXE14, JHE-related, JHER, kidney bean esterase, Kidney microsomal carboxylesterase, LcalphaE7, LIP4, LipA, LipC, Liver microsomal carboxylesterase, malathion carboxylesterase, MAP esterase, MCE, ME, MeIAA esterase, MeSA esterase, methylbutyrase, methylbutyrate esterase, mfCES2v3, Microsomal palmitoyl-CoA hydrolase, monobutyrase, Monocyte/macrophage serine esterase, More, mouse carboxylesterase, MT2282, NaM1, NaM2, NaM3, Non-specific carboxylesterase, nonspecific carboxylesterase, ODE, odorant degradation enzyme, P186_1588, PA3859, PepEST, PF2001, Pf2001 esterase, PI 5.5 esterase, PI 6.1 esterase, pig liver esterase, PLE, PMPMEase, pnbA, PnbA1, PnbA2, polyisoprenylated methylated protein methyl esterase, porcine liver carboxylesterase, PPE, PPMTase, PrbA, prenylated methylated protein methyl esterase, procaine esterase, Proline-beta-naphthylamidase, propionyl esterase, pyrethroid-hydrolysing esterase, Rsp3690, SABP2's methyl salicylate esterase, salicylic acid-binding protein 2, SeE, serine protease-like, serine-type carboxylesterase, SexiCXE10, SiRe0290, SshEstI, SshEstI esterase, Sso P1 carboxylesterase, Sso-EST1, SSO2493, SSO2517, SSoP1 CE, SsoPEst, ST0071, ST2026, STK_00710, Sto-Est, TGH, triacetin esterase, triacylglycerol hydrolase, vitamin A esterase, VmoLac

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.1 carboxylesterase

Cloned

Cloned on EC 3.1.1.1 - carboxylesterase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
CES2, recombinant expression of His10-tagged wild-type and mutant enzymes in HEK-293T cells, the enzyme is secreted to the cell medium
CXE1 is expressed in Escherichia coli M15 cells
-
DNA and amino acid sequence determination and analysis, genetic organization, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged wild-type and truncated mutant EstPS1s in Escherichia coli, the mutant enzymes are found predominantly in inclusion bodies, the soluble enzyme is secreted
DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli BL21(DE3)
DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged BSE01701 esterase in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
DNA sequence determination and analysis
DNA sequence determination and analysis, genetic organization, expression in Escherichia coli
expressed as a fusion protein in Escherichia coli
expressed in baculovirus-infected High Five insect cells
-
expressed in baculovirus-infected Spodoptera frugiperda Sf21 cells
-
expressed in Escherichia coli
expressed in Escherichia coli as a His-tagged protein
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21(DE3)
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli JM109 and BL21 (DE3) cells
expressed in Escherichia coli M15[pREP4] cells
expressed in Escherichia coli strain BL21
expressed in Escherichia coli strain JM109
O76177; Q95UJ4
expressed in Flp-In-293 cells
expressed in HepG2 cells
-
expressed in High Five cells of Trichoplusia ni
-
expressed in Pichia pastoris strain GS115 and Aspergillus niger strain M54
-
expressed in sf21 cells
-
expressed in Spodoptera frugiperda cells and in U373MG cells
expressed in Trichoplusia ni larvae
expression as glutathione S-transferase fusion protein in Escherichia coli
-
expression in Arabidopsis thaliana
-
expression in Escherichia coli
expression in Escherichia coli as a 6His-tagged fusion protein
expression in Escherichia coli as thioredoxin-free form (EcSisEstA I) and as thioredoxin-EstA fusion protein (EcSisEstA II). Expression in Sulfolobus islandicus E233S, a DELTASpyrEF DELTAlacS mutant constructed from Sulfolobus islandicus REY15A. Expressing the enzyme in a mesophilic host renders the enzyme much less thermoactive
expression in Escherichia coli by using a His-tag expression vector
expression in Escherichia coli JM83
-
expression in Escherichia coli of chimeras of a carboxylesterase (EC 3.1.1.1) from Archaeoglobus fulgidus and an acylpeptide hydrolase (EC 3.4.19.1) from Aeropyrum pernix K1
-
expression in Escherichia coli, His-tag
expression in Escherichia coli, N-terminal His-tag
-
expression in Escherichia coli, with c-terminal His-tag and wizthout the N-terminal hydrophobic region
-
expression in MCF-7 cell and U-373MG glioblastoma cell
-
expression in sf21 cell
expression in Sf9 cell
expression of the selenomethionine enriched enzyme in Escherichia coli methionine auxotroph strain B834 (DE3) in supplemented M9 medium
functional heterologous expression of the enzyme in Pichia pastoris was only possible after elimination of the C-terminal endoplasmic reticulum retention signal sequence His-Ala-Glu-Leu, the recombinant enzyme is secreted into the medium
-
functionally expressed in Escherichia coli as His-tagged protein
gene At3g10870 or AtMES17, phylogenetic analysis
gene bioHe, DNA and amino acid sequence determination and analysis, sequence compariosns, overview
-
gene bioHs, cloned from the genomic DNA library, DNA and amino acid sequence determination and analysis, expression of the HIs-tagged enzyme in Escherichia coli strain JM109
gene caesCCR11, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression in Escherichia coli strain BL21(DE3)
gene CES2, genotyping of CES2 genetic polymorphisms in Japanese population, overview
-
gene EST1, DNA and amino acid sequence determination and analysis, exon-intron organization, sequence comparisons and phylogenetic analysis, quantitative reverse transcription PCR enzyme expression analysis, recombinant expression in Spodoptera frugiperda Sf9 insect cells
gene estA, DNA sequence determination and analysis, expression in Escherichia coli
-
gene estA, expression as His-tagged enzyme in Escherichia coli
-
gene estB, recombinant expression of His-tagged enzyme in Escherichia coli strain DH5alpha
gene estC, DNA sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli
gene fpbH, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene mhg, recombinant expression of wild-type and mutant enzymes in Escherichia coli
-
gene PsEst1, DNA and amino acid sequence determination and analysis, overexpression of the cold-adapted enzyme in Escherichia coli BL21(DE3), formation of inclusion bodies
gene SexiCXE10, DNA and amino acid sequence determination and analysis, phylogenetic tree, quantitative PCR expression analysis, recombinant expression of N-terminally His-tagged enzyme with Kozak sequences at N-terminus in High-Five cells of Trichoplusia ni using Autographa californica nucleopolyhedrovirus transfection
-
genetic analysis
-
genetic analysis, expression in Escherichia coli as N-terminally His-tagged enzyme
His-tagged expression in Escherichia coli, with coexpresssion of chaperones GroEL and GroES. Expression in Pichia pastoris
-
MES family phylogenetic tree
-
mutant enzymes are expressed in Escherichia coli JM105 cells
-
overexpressed from Escherichia coli BL21
overexpressed in Escherichia coli
overexpression in Escherichia coli
overexpression in Escherichia coli as a soluble catalytically active protein
-
overexpression in Escherichia coli BL21(DE3)
-
overexpression in Escherichia coli with fusion His-tags at the C-terminus
overexpression of human liver enzyme in a human cell line
overexpression of wild-type and mutants in Escherichia coli BL21(DE3)
-
overexpression system in Escherichia coli
overproduction in Escherichia coli
recombinant expression of enzyme CES1 in Spodoptera frugiperda Sf21 cells
F1P6W8, Q95N05
recombinant expression of enzyme CES2 in Spodoptera frugiperda Sf21 cells
F1P6W8, Q95N05
recombinant expression of wild-type and mutant His-tagged enzyme CES1 in Escherichia coli strain BL21(DE3) in inclusion bodies, the recombinant CES1 protein is expressed differently when grown at different temperatures, expression of CES1 grown at 25°C was much higher than in cells grown at 37°C
sequence comparisons
-
simultaneous expression of carboxylesterase B1 and organophosphorus hydrolase EC 3.1.8.1 in Escherichia coli
Culex sp.
-
synthetic construct NaM1, cloned from Namib soil hypolith metagenomic data set, i.e. hypolithome, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
-
synthetic construct NaM2, cloned from Namib soil hypolith metagenomic data set, i.e. hypolithome, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
-
the gene that has a deletion of about 60 residues at the N terminus(SSODELTAN) is expressed in Escherichia coli