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1.8.98.2: sulfiredoxin

This is an abbreviated version!
For detailed information about sulfiredoxin, go to the full flat file.

Word Map on EC 1.8.98.2

Reaction

peroxiredoxin-(S-hydroxy-S-oxocysteine)
+
ATP
+ 2 R-SH =
peroxiredoxin-(S-hydroxycysteine)
+
ADP
+
phosphate
+
R-S-S-R

Synonyms

AtSrx, cysteine-sulfinic acid reductase, neoplastic progression 3, peroxiredoxin-(S-hydroxy-S-oxocysteine) reductase, protein cysteine sulfinic acid reductase, Srx, Srx1, Srxn1, sulfiredoxin, sulfiredoxin 1, sulfiredoxin-1, sulphiredoxin

ECTree

     1 Oxidoreductases
         1.8 Acting on a sulfur group of donors
             1.8.98 With other, known, physiological acceptors
                1.8.98.2 sulfiredoxin

Cloned

Cloned on EC 1.8.98.2 - sulfiredoxin

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, expression of N-terminally His6-tagged wild-type Srx and mutants in Escherichia coli strain BL21(DE3)
-
expressed in Escherichia coli BL21(DE3) cells
-
expressed in HEK293 cells and Escherichia coli
expressed in Saccharomyces cerevisiae
expression in Escherichia coli
for expression in Escherichia coli BL21DE3 cells
-
gene SRX, overexpression of recombinant His-tagged full-length enzyme in Escherichia coli strain BL21(DE3)
into the vector pcDNA3
-
into the vector pET14b for expression in Escherichia coli BL21 cells
-
the 5' flanking region of the human Srx1 promoter region, -3664 - +19, is ligated into the vector pCR2.1
-
the vectors pcDNA3 and pFLAG-CMV2 are used, a leader sequence is cloned into the N-terminus coding region of Srx
-
transient expression of an AtSrx-GFP fusion in Nicotiana tabacum leaves
transient recombinant expression of the FLAG-tagged enzyme in different cell types, including HEK293T, H226, and A549 cells, recombinant expression of FLAG-tagged Srx mutant C99A mutant in HEK-293T cells. The Srx-protein complex is pulled down by anti-FLAG immunoprecipitation (IP), and protein identities are determined by mass spectrometry. Among the handful of proteins identified, Prx4 is the most abundant protein, and TXNDC5 is the second most abundant protein that is specifically pulled down by anti-FLAG IP in all cell types. c-Myc-tagged TXNDC5 or its cysteine-specific mutants are expressed in HEK-293T-FLAG-Srx cells