1.8.98.1: dihydromethanophenazine:CoB-CoM heterodisulfide reductase

This is an abbreviated version!
For detailed information about dihydromethanophenazine:CoB-CoM heterodisulfide reductase, go to the full flat file.

Reaction

Synonyms

Co:CoM-S-S-CoB oxidoreductase system, CO:heterodisulfide oxidoreductase system, CoB-S-S-CoM reductase, Coenzyme B-Coenzyme M heterodisulfide reductase, coenzyme F420:heterodisulfide oxidoreductase, CoM-S-S-HTP reductase, DsrE2A, DsrK, F420H2-dependent CoM-S-S-HTP reductase, F420H2-dependent heterodisulfide reductase, Fdx:CoMS-SCoB oxidoreductase, ferredoxin- and F420H2-dependent, electron-bifurcating, heterodisulfide reductase, H2:heterodisulfide oxidoreductase, HDR, HdrA, hdrA2B2C2, HdrABC, HdrB, HdrB1, HdrB2, HdrC, HdrC1, HdrC2, HdrD, heterodisulfide reductase, More, SdhC, soluble heterodisulfide reductase, Vlud

ECTree

     1 Oxidoreductases

         1.8 Acting on a sulfur group of donors

             1.8.98 With other, known, physiological acceptors

                1.8.98.1 dihydromethanophenazine:CoB-CoM heterodisulfide reductase

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
2	Activating Compound
2040	AA Sequence
2	CAS Registry Number
8	Cloned(Commentary)
24	Cofactor
1	Crystallization (Commentary)
4	Expression
11	General Information
1	General Stability
10	Inhibitors
20	KM Value [mM]
18	Localization
20	Metals/Ions
20	Molecular Weight [Da]
29	Natural Substrates/ Products (Substrates)
152	Organism
2	Oxidation Stability
6	Pathway
28	PDB ID
4	pH Optimum
1	pH Range
1	pI Value
1	Posttranslational Modification
3	Protein Variants
20	Purification (Commentary)
2	Reaction
5	Reaction Type
51	Reference
5	Source Tissue
14	Specific Activity [micromol/min/mg]
2	Storage Stability
81	Substrates and Products (Substrate)
16	Subunits
97	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
2	Temperature Stability [°C]
2	Turnover Number [1/s]

Substrates Products

print visible entries

print all entries

Substrates Products on EC 1.8.98.1 - dihydromethanophenazine:CoB-CoM heterodisulfide reductase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide show all columns Go to Substrate/Product Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

coenzyme B + 6-mercaptohexanoyl-L-threonine phosphate + methylene blue

? + methylene blue

2 entries

coenzyme B + coenzyme M + methanophenazine

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + dihydromethanophenazine

6 entries

coenzyme B + coenzyme M + methanophenazine

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-O3-phospho-L-threonine + dihydromethanophenazine

3 entries

coenzyme B + coenzyme M + methanophenazine

N-[7-[(2-sulfoethyl)dithio]heptanoyl]-O3-phospho-L-threonine + dihydromethanophenazine

4 entries

coenzyme B + coenzyme M + methylene blue

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced methylene blue

Methanosarcina barkeri

-

-

655400

-

-

?

coenzyme B + coenzyme M + reduced ferredoxin + H+ + coenzyme F420

coenzyme B-coenzyme M disulfide + ferredoxin + H2 + reduced coenzyme F420

Methanosarcina acetivorans

-

-

743210

-

-

?

coenzyme B-coenzyme M disulfide + dihydromethanophenazine

coenzyme B + coenzyme M + methanophenazine

2 entries

coenzyme B-coenzyme M disulfide + reduced benzyl viologen

coenzyme B + coenzyme M + benzyl viologen

2 entries

coenzyme B-coenzyme M disulfide + reduced ferredoxin

coenzyme B + coenzyme M + ferredoxin

2 entries

coenzyme B-coenzyme M disulfide + reduced methyl viologen

coenzyme B + coenzyme M + methyl viologen

2 entries

CoM-S-S-CoB + ?

CoM-SH + CoB-SH + ?

3 entries

DsrC protein disulfide + dihydromethanophenazine

DsrC protein + methanophenazine

2 entries

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + coenzyme F420H2

coenzyme B + coenzyme M + coenzyme F420

4 entries

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + dihydro-2-hydroxyphenazine

coenzyme B + coenzyme M

Methanosarcina mazei

-

-

655825

-

-

?

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + dihydromethanophenazine

coenzyme B + coenzyme M + methanophenazine

2 entries

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2

coenzyme B + coenzyme M

12 entries

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced 2-bromophenazine

coenzyme B + coenzyme M + 2-bromophenazine

Methanosarcina mazei

-

-

440299

-

-

?

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced 2-hydroxyphenazine

coenzyme B + coenzyme M + 2-hydroxyphenazine

Methanosarcina mazei

-

-

440299

-

-

?

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced benzyl viologen

coenzyme B + coenzyme M + benzyl viologen

2 entries

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced benzylviologen

coenzyme B + coenzyme M + benzylviologen

Methanothermobacter thermautotrophicus

-

-

639391

-

-

?, r

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced benzylviologen

coenzyme B + coenzyme M + oxidized benzyl viologen

Methanosarcina barkeri

-

reaction intermediate is a [4Fe-4S]3+ cluster that is coordinated by one of the cysteines of nearby active-site disulfide or by the sulfur of coenzyme M

655416

-

-

?

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced cytochrome b

coenzyme B + coenzyme M + oxidized cytochrome b

2 entries

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced methyl viologen

coenzyme B + coenzyme M + methyl viologen

Methanosarcina thermophila

-

ping-pong mechanism

639394

-

-

?

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced methylene blue

coenzyme B + coenzyme M + methylene blue

2 entries

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced methylviologen

coenzyme B + coenzyme M + methyl viologen

2 entries

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced methylviologen

coenzyme B + coenzyme M + methylviologen

2 entries

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced methylviologen

coenzyme B + coenzyme M + oxidized methylviologen

Methanosarcina thermophila

-

-

655823

-

-

?

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced phenazine-1-carboxylic acid

coenzyme B + coenzyme M + phenazine-1-carboxylic acid

Methanosarcina mazei

-

-

440299

-

-

?

additional information

?

-

8 entries

coenzyme B + 6-mercaptohexanoyl-L-threonine phosphate + methylene blue

? + methylene blue

Methanothermobacter thermautotrophicus

-

-

639391

-

-

ir

coenzyme B + 6-mercaptohexanoyl-L-threonine phosphate + methylene blue

? + methylene blue

Methanothermobacter thermautotrophicus Marburg / DSM 2133

-

-

639391

-

-

ir

coenzyme B + coenzyme M + methanophenazine

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + dihydromethanophenazine

Methanosarcina barkeri

-

-

667866

-

-

?

coenzyme B + coenzyme M + methanophenazine

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + dihydromethanophenazine

Methanosarcina mazei

-

-

440299

-

-

?

coenzyme B + coenzyme M + methanophenazine

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + dihydromethanophenazine

Methanosarcina thermophila

-

-

639393, 639394, 667866

-

-

?

coenzyme B + coenzyme M + methanophenazine

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + dihydromethanophenazine

Methanothermobacter marburgensis

-

-

667866

-

-

?

coenzyme B + coenzyme M + methanophenazine

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + dihydromethanophenazine

Methanothermobacter thermautotrophicus

-

-

639391

-

-

?

coenzyme B + coenzyme M + methanophenazine

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + dihydromethanophenazine

Methanothermobacter thermautotrophicus Marburg / DSM 2133

-

-

639391

-

-

?

coenzyme B + coenzyme M + methanophenazine

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-O3-phospho-L-threonine + dihydromethanophenazine

Methanococcus maripaludis

-

-

713413

-

-

?

coenzyme B + coenzyme M + methanophenazine

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-O3-phospho-L-threonine + dihydromethanophenazine

Methanococcus maripaludis MM1265

-

-

713413

-

-

?

coenzyme B + coenzyme M + methanophenazine

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-O3-phospho-L-threonine + dihydromethanophenazine

Methanosarcina acetivorans

-

-

713055

-

-

?

coenzyme B + coenzyme M + methanophenazine

N-[7-[(2-sulfoethyl)dithio]heptanoyl]-O3-phospho-L-threonine + dihydromethanophenazine

Acidithiobacillus ferrooxidans

-

-

741554

-

-

?

coenzyme B + coenzyme M + methanophenazine

N-[7-[(2-sulfoethyl)dithio]heptanoyl]-O3-phospho-L-threonine + dihydromethanophenazine

Acidithiobacillus ferrooxidans DSM 16786

-

-

741554

-

-

?

coenzyme B + coenzyme M + methanophenazine

N-[7-[(2-sulfoethyl)dithio]heptanoyl]-O3-phospho-L-threonine + dihydromethanophenazine

Acidithiobacillus thiooxidans

-

-

741554

-

-

?

coenzyme B + coenzyme M + methanophenazine

N-[7-[(2-sulfoethyl)dithio]heptanoyl]-O3-phospho-L-threonine + dihydromethanophenazine

Acidithiobacillus thiooxidans DSM 17318

-

-

741554

-

-

?

coenzyme B-coenzyme M disulfide + dihydromethanophenazine

coenzyme B + coenzyme M + methanophenazine

Desulfovibrio vulgaris

-

-

742382

-

-

?

coenzyme B-coenzyme M disulfide + dihydromethanophenazine

coenzyme B + coenzyme M + methanophenazine

Desulfovibrio vulgaris ATCC 29579

-

-

742382

-

-

?

coenzyme B-coenzyme M disulfide + reduced benzyl viologen

coenzyme B + coenzyme M + benzyl viologen

Methanomassiliicoccus luminyensis

-

-

742500

-

-

?

coenzyme B-coenzyme M disulfide + reduced benzyl viologen

coenzyme B + coenzyme M + benzyl viologen

Methanomassiliicoccus luminyensis DSM 25720

-

-

742500

-

-

?

coenzyme B-coenzyme M disulfide + reduced ferredoxin

coenzyme B + coenzyme M + ferredoxin

Methanomassiliicoccus luminyensis

-

-

742500

-

-

?

coenzyme B-coenzyme M disulfide + reduced ferredoxin

coenzyme B + coenzyme M + ferredoxin

Methanomassiliicoccus luminyensis DSM 25720

-

-

742500

-

-

?

coenzyme B-coenzyme M disulfide + reduced methyl viologen

coenzyme B + coenzyme M + methyl viologen

Methanomassiliicoccus luminyensis

-

-

742500

-

-

?

coenzyme B-coenzyme M disulfide + reduced methyl viologen

coenzyme B + coenzyme M + methyl viologen

Methanomassiliicoccus luminyensis DSM 25720

-

-

742500

-

-

?

CoM-S-S-CoB + ?

CoM-SH + CoB-SH + ?

Methanosarcina barkeri

-

-

667866

-

-

?

CoM-S-S-CoB + ?

CoM-SH + CoB-SH + ?

Methanosarcina thermophila

-

-

667866

-

-

?

CoM-S-S-CoB + ?

CoM-SH + CoB-SH + ?

Methanothermobacter marburgensis

-

-

667866

-

-

?

DsrC protein disulfide + dihydromethanophenazine

DsrC protein + methanophenazine

Desulfovibrio vulgaris

-

-

742382

-

-

r

DsrC protein disulfide + dihydromethanophenazine

DsrC protein + methanophenazine

Desulfovibrio vulgaris ATCC 29579

-

-

742382

-

-

r

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + coenzyme F420H2

coenzyme B + coenzyme M + coenzyme F420

Methanococcus voltae

-

-

654386

-

-

?

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + coenzyme F420H2

coenzyme B + coenzyme M + coenzyme F420

Methanolobus tindarius

-

-

655532

-

-

?

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + coenzyme F420H2

coenzyme B + coenzyme M + coenzyme F420

Methanosarcina mazei

-

-

655532, 657172

-

-

?

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + coenzyme F420H2

coenzyme B + coenzyme M + coenzyme F420

Methanosarcina mazei

-

reaction is coupled with the transfer of protons across the membrane of washed vesicles, which drives ATP formation from ADP

657172

-

-

?

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + dihydromethanophenazine

coenzyme B + coenzyme M + methanophenazine

Methanosarcina thermophila

-

-

639394

-

-

?

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + dihydromethanophenazine

coenzyme B + coenzyme M + methanophenazine

Methanosarcina thermophila

-

ping-pong mechanism

639394

-

-

?

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2

coenzyme B + coenzyme M

Methanosarcina barkeri

-

-

655396, 655400

-

-

?

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2

coenzyme B + coenzyme M

Methanosarcina barkeri

-

energy-conserving step in methanohenic archaea

655396

-

-

?

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2

coenzyme B + coenzyme M

Methanosarcina mazei

-

-

655825

-

-

?

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2

coenzyme B + coenzyme M

Methanosarcina mazei

-

reaction is coupled with transfer of protons across the cytoplasmic membrane. The electrochemical proton gradient thereby generated drives ATP synthesis from ADP plus phosphate. Two different proton-translocating segments are present in the H2:heterodisulfide oxidoreductase system. The first involves the 2-hydroxyphenazine-dependent hydrogenase, and the second involves the heterodisulfide reductase

655825

-

-

?

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2

coenzyme B + coenzyme M

Methanosarcina mazei

-

reaction is independent of coenzyme F420. Reaction is coupled to proton translocation across the cytoplasmic membrane into the lumen of the everted vesicle. The transmembrane electrochemical gradient thereby generated unduces ATP synthesis from ADP + phosphate

654385

-

-

?

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2

coenzyme B + coenzyme M

Methanothermobacter thermautotrophicus

-

-

655398, 655471, 655531

-

-

?

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2

coenzyme B + coenzyme M

Methanothermobacter thermautotrophicus

-

energy-conserving step

655398

-

-

?

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2

coenzyme B + coenzyme M

Methanothermobacter thermautotrophicus

-

the D-enantiomer of the heterodisulfide is reduced at approximately 35% of the rate observed for the L-form

639391

-

-

?

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2

coenzyme B + coenzyme M

Methanothermobacter thermautotrophicus Marburg / DSM 2133

-

the D-enantiomer of the heterodisulfide is reduced at approximately 35% of the rate observed for the L-form

639391

-

-

?

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2

coenzyme B + coenzyme M

Methanothermobacter thermautotrophicus Marburg / DSM 2133

-

-

655398

-

-

?

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2

coenzyme B + coenzyme M

Methanothermobacter thermautotrophicus Marburg / DSM 2133

-

energy-conserving step

655398

-

-

?

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2

coenzyme B + coenzyme M

Methanothermobacter thermautotrophicus Marburg / DSM 2133

-

-

655531

-

-

?

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced benzyl viologen

coenzyme B + coenzyme M + benzyl viologen

Methanosarcina barkeri

-

-

655396

-

-

?

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced benzyl viologen

coenzyme B + coenzyme M + benzyl viologen

Methanothermobacter marburgensis

-

reaction intermediate is a [4Fe-4S]3+ cluster that is coordinated by one of the cysteines of nearby active-site disulfide or by the sulfur of coenzyme M

655416

-

-

?

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced cytochrome b

coenzyme B + coenzyme M + oxidized cytochrome b

Methanosarcina thermophila

-

-

655823

-

-

?

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced cytochrome b

coenzyme B + coenzyme M + oxidized cytochrome b

Methanosarcina thermophila

-

electron transfer by a CO dehydrogenase in complex with the heterodisulfide oxidoreductase, no H2 uptake in vivo, reaction is part of the overall reduction of acetate to methane and H2O, also providing a proton-translocation redox system to drive ATP formation

655823

-

-

?

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced methylene blue

coenzyme B + coenzyme M + methylene blue

Methanosarcina barkeri

-

-

639391

-

-

r

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced methylene blue

coenzyme B + coenzyme M + methylene blue

Methanothermobacter thermautotrophicus

-

-

639391

-

-

r

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced methylviologen

coenzyme B + coenzyme M + methyl viologen

Methanosarcina barkeri

-

-

655400

-

-

?

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced methylviologen

coenzyme B + coenzyme M + methyl viologen

Methanosarcina mazei

-

-

440299

-

-

?

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced methylviologen

coenzyme B + coenzyme M + methylviologen

Methanococcus voltae

-

-

654386

-

-

?

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced methylviologen

coenzyme B + coenzyme M + methylviologen

Methanosarcina thermophila

-

-

639393

-

-

?

additional information

?

-

Methanococcus maripaludis

-

Hdr complexes with F420-nonreducing hydrogenase, formate dehydrogenase, and formyl-MFR dehydrogenase, model of complex of proteins that interact with Hdr, overview

713413

-

-

?

additional information

?

-

Methanococcus maripaludis MM1265

-

Hdr complexes with F420-nonreducing hydrogenase, formate dehydrogenase, and formyl-MFR dehydrogenase, model of complex of proteins that interact with Hdr, overview

713413

-

-

?

additional information

?

-

Methanolobus tindarius

-

no activity with the homodisulfides of coenzyme M and coenzyme B

655532

-

-

?

additional information

?

-

Methanosarcina mazei

-

no activity with the homodisulfides of coenzyme M and coenzyme B

655532

-

-

?

additional information

?

-

Methanothermobacter thermautotrophicus

-

homodisulfides of coenzyme M and coenzyme B are not reduced

639391

-

-

?

additional information

?

-

Methanothermobacter thermautotrophicus

-

the enzyme does not catalyze the reduction of N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine with NADH, NADPH or reduced coenzyme F420

639391

-

-

?

additional information

?

-

Methanothermobacter thermautotrophicus Marburg / DSM 2133

-

homodisulfides of coenzyme M and coenzyme B are not reduced

639391

-

-

?

additional information

?

-

Methanothermobacter thermautotrophicus Marburg / DSM 2133

-

the enzyme does not catalyze the reduction of N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine with NADH, NADPH or reduced coenzyme F420

639391

-

-

?