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1.8.5.4: bacterial sulfide:quinone reductase

This is an abbreviated version!
For detailed information about bacterial sulfide:quinone reductase, go to the full flat file.

Word Map on EC 1.8.5.4

Reaction

n HS- + n quinone =

Polysulfide
+ n quinol

Synonyms

CmSQR, CpSQR, CT1087, HMT2, III SQR, membrane-bound sulfide:quinone oxidoreductases, SQOR, SQR, Sqrdl, sqrF, Suden_1879, Suden_2082, Suden_619, sulfide quinone oxidoreductase, sulfide quinone reductase, sulfide-quinone oxidoreductase, sulfide-quinone reductase, sulfide: quinone oxidoreductase, sulfide:decylubiquinone oxidoreductase, sulfide:quinone oxidoreductase, sulfidequinone reductase-like protein, TrSqrF, type I SQR, type III sulfide:quinone oxidoreductase, type VI sulfide:quinone oxidoreductase

ECTree

     1 Oxidoreductases
         1.8 Acting on a sulfur group of donors
             1.8.5 With a quinone or similar compound as acceptor
                1.8.5.4 bacterial sulfide:quinone reductase

Purification

Purification on EC 1.8.5.4 - bacterial sulfide:quinone reductase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, Hi-Trap column chromatography, and Superdex 200 gel filtration
-
ammonium sulfate precipitation, phenyl-Toyopearl column chromatography, and TSKgel G3000 gel filtration
-
ammonium sulfate precipitation, phenyl-TSK column chromatography, T SK-gel filtration and analytical gel filtration
-
DEAE-cellulose column chromatography and Mono Q column chromatography
HiTrap IMAC column chromatography and Q Sepharose column chromatography
MonoQ column chromatography and TSK 4000 gel filtration
MonoQ column chromatography, TSK-1 gel filtration, and TSK-2 gel filtration
Ni-NTA column chromatography
Ni-NTA column chromatography, gel filtration
-
nickel affinity column chromatography
nickel affinity column chromatography and Superdex 200 gel filtration
nickel affinity column chromatography, and Superdex 200 gel filtration
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain C43(DE3) by nickel affinity chromatography, dialysis, ultracentrifugation at 230000 x g, followed by solubilization with dodecyl-beta-D-maltoside and another step of nickel affinity chromatography
-
recombinant His6-tagged wild-type enzyme and mutants from Escherichia coli by nickel affinity chromatography
recombinant StrepII-tagged enzyme point mutants from Thiocapsa roseopersicina cells by affinity chromatography to homogeneity