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1.8.5.1: glutathione dehydrogenase (ascorbate)

This is an abbreviated version!
For detailed information about glutathione dehydrogenase (ascorbate), go to the full flat file.

Word Map on EC 1.8.5.1

Reaction

2 glutathione +

dehydroascorbate
=
glutathione disulfide
+
ascorbate

Synonyms

At1g19570, At1g75270, At5g16710, AtDHAR, AtDHAR1, AtDHAR2, AtDHAR3, CrDHAR1, DasA reductase, dehydroascorbate reductase, dehydroascorbate reductase 2, dehydroascorbic acid reductase, dehydroascorbic reductase, dehydrogenase, glutathione (ascorbate), DHA reductase, DHA-R, DHAR, DHAR1, DHAR2, DHAR3, DHAR3a, DHAR4, DHAR5, dioscorin, GDOR, glutathione dehydroascorbate reductase, glutathione-dependent dehydroascorate reductase, glutathione:dehydroascorbic acid oxidoreductase, GRX1, GRX2, GSH-DHAR, GSH:DHA-oxidoreductase, GSTO, GSTO1, GSTO1-1, GSTO2, GSTO2-2, LcDHAR, metallothionein-1, metallothionein-2, More, MT-I, MT-II, nlGSTO, OsDHAR, OsDHAR1, PbDHAR, PgDHAR1, PtrDHAR1, PtrDHAR2, PtrDHAR3A, PtrDHAR3B, SPD1

ECTree

     1 Oxidoreductases
         1.8 Acting on a sulfur group of donors
             1.8.5 With a quinone or similar compound as acceptor
                1.8.5.1 glutathione dehydrogenase (ascorbate)

Purification

Purification on EC 1.8.5.1 - glutathione dehydrogenase (ascorbate)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
functional TcGrx is purified by Ni2+-nitrilotriacetic acid sepharose
-
native enzyme partially from cytosolic fraction by centrifugation and dialysis
Ni Sepharose column chromatography
Ni-agarose affinity chromatography
Ni-NTA column chromatography
Ni-NTA column chromatography and Superdex 200 gel filtration
Ni-NTA resin column chromatography
Ni2+-chealting NiNTA Superflow column chromatography
-
recombinant enzyme
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant wild-type and mutant enzymes from Escherichia coli
using affinity chromatography