Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.019 - 2.5
dehydroascorbate
additional information
additional information
-
0.019
dehydroascorbate
-
mutant enzyme C9S
0.026
dehydroascorbate
-
mutant enzyme C23S
0.05
dehydroascorbate
pH and temperature not specified in the publication, chloroplastic isozyme
0.053
dehydroascorbate
-
wild-type enzyme
0.058
dehydroascorbate
-
-
0.06
dehydroascorbate
-
recombinant enzyme form DHAR-1
0.068
dehydroascorbate
-
isoform DHAR1, at pH 6.5 and 25°C
0.068
dehydroascorbate
-
isoform DHAR2, at pH 6.5 and 25°C
0.07
dehydroascorbate
-
enzyme form DHAR-a
0.07
dehydroascorbate
pH not specified in the publication, temperature not specified in the publication
0.07
dehydroascorbate
-
isoform DHAR2, at pH 7.8 and 25°C
0.07
dehydroascorbate
pH and temperature not specified in the publication, chloroplastic isozyme DHAR1
0.07
dehydroascorbate
pH and temperature not specified in the publication, cytosolic isozyme
0.07
dehydroascorbate
-
pH and temperature not specified in the publication, plastidic isozyme DHAR2
0.08
dehydroascorbate
-
enzyme form DHAR-b
0.08
dehydroascorbate
Vmax: 34.12 micromol/min/mg
0.08
dehydroascorbate
pH and temperature not specified in the publication, cytosolic isozyme
0.09
dehydroascorbate
pH 8.0, 22°C, recombinant wild-type enzyme
0.09
dehydroascorbate
pH and temperature not specified in the publication, cytosolic isozyme DHAR2
0.11
dehydroascorbate
-
isoform DHAR1, at pH 7.8 and 25°C
0.11
dehydroascorbate
-
pH and temperature not specified in the publication, cytosolic isozyme DHAR1
0.113
dehydroascorbate
-
isoform DHAR1, at pH 6.5 and 25°C
0.115
dehydroascorbate
-
isoform DHAR1, at pH 6.5 and 25°C
0.118
dehydroascorbate
-
isoform DHAR2, at pH 6.5 and 25°C
0.119
dehydroascorbate
-
isoform DHAR4, at pH 6.5 and 25°C
0.13
dehydroascorbate
mutant D72A, pH not specified in the publication, temperature not specified in the publication
0.13
dehydroascorbate
pH and temperature not specified in the publication, cytosolic isozyme
0.1334
dehydroascorbate
at pH 7.0 and 40°C
0.15
dehydroascorbate
-
isoform DHAR3, at pH 6.5 and 25°C
0.16
dehydroascorbate
pH and temperature not specified in the publication, chloroplastic isozyme DHAR3B
0.17
dehydroascorbate
pH not specified in the publication, temperature not specified in the publication
0.18
dehydroascorbate
pH and temperature not specified in the publication, cytosolic isozyme DHAR3A
0.19
dehydroascorbate
25°C
0.19
dehydroascorbate
pH and temperature not specified in the publication, cytosolic isozyme
0.21
dehydroascorbate
-
-
0.23
dehydroascorbate
wild-type, pH not specified in the publication, temperature not specified in the publication
0.23
dehydroascorbate
pH and temperature not specified in the publication, chloroplastic isozyme DHAR1
0.23
dehydroascorbate
pH and temperature not specified in the publication, cytosolic isozyme DHAR2
0.245
dehydroascorbate
-
-
0.248
dehydroascorbate
-
isoform DHAR2, at pH 6.5 and 25°C
0.26
dehydroascorbate
-
-
0.32
dehydroascorbate
-
glutathione-dependent dehydroascorbate reductase activity of thioltransferase (glutaredoxin)
0.32
dehydroascorbate
recombinant mutant L225A, pH 8.0, 30°C
0.34
dehydroascorbate
-
-
0.35
dehydroascorbate
-
-
0.35
dehydroascorbate
-
rice recombinant DHAR
0.35
dehydroascorbate
pH and temperature not specified in the publication, cytosolic isozyme
0.39
dehydroascorbate
-
-
0.39
dehydroascorbate
pH not specified in the publication, temperature not specified in the publication
0.39
dehydroascorbate
pH 7.0, temperature not specified in the publication
0.39
dehydroascorbate
pH and temperature not specified in the publication, cytosolic isozyme
0.43
dehydroascorbate
mutant containing six cysteine-to-serine mutations, with C-terminal residues FGLC deleted, pH 6.9, 30°C
0.48
dehydroascorbate
pH not specified in the publication, temperature not specified in the publication
0.48
dehydroascorbate
pH and temperature not specified in the publication, cytosolic isozyme DHAR3
0.5
dehydroascorbate
allelic variant D142 of GSTO2-2
0.5
dehydroascorbate
allelic variant N142 of GSTO2-2
0.51
dehydroascorbate
-
glutathione-dependent dehydroascorbate reductase activity of thioltransferase (glutaredoxin), C25S mutant
0.51
dehydroascorbate
wild-type, pH 6.9, 30°C
0.52
dehydroascorbate
mutant containing six cysteine-to-serine mutations with the C-terminal cysteine residue deleted, pH 6.9, 30°C
0.53
dehydroascorbate
mutant with C-terminal residues FGLC deleted, pH 6.9, 30°C
0.55
dehydroascorbate
recombinant mutant F28A, pH 8.0, 30°C
0.58
dehydroascorbate
-
-
0.6
dehydroascorbate
recombinant wild-type enzyme, pH 8.0, 30°C
0.6
dehydroascorbate
recombinant mutant R176A, pH 8.0, 30°C
0.67
dehydroascorbate
recombinant mutant P30A, pH 8.0, 30°C
0.7
dehydroascorbate
mutant S73A, pH not specified in the publication, temperature not specified in the publication
0.77
dehydroascorbate
recombinant mutant C29A, pH 8.0, 30°C
0.81
dehydroascorbate
pH and temperature not specified in the publication, cytosolic isozyme
0.97
dehydroascorbate
mutant K8A, pH not specified in the publication, temperature not specified in the publication
1.85
dehydroascorbate
-
-
2
dehydroascorbate
pH 6.3, temperature not specified in the publication
0.04
glutathione
pH and temperature not specified in the publication, cytosolic isozyme
0.04167
glutathione
at pH 7.0 and 40°C
0.27
glutathione
pH 8.0, 22°C, recombinant wild-type enzyme
0.74
glutathione
-
isoform DHAR1, at pH 6.5 and 25°C
0.84
glutathione
pH and temperature not specified in the publication, cytosolic isozyme
0.96
glutathione
pH not specified in the publication, temperature not specified in the publication
1
glutathione
-
rice recombinant DHAR
1.03
glutathione
Vmax: 136.20 micromol/min/mg
1.03
glutathione
pH and temperature not specified in the publication, cytosolic isozyme
1.079
glutathione
-
isoform DHAR3, at pH 6.5 and 25°C
1.1
glutathione
pH and temperature not specified in the publication, chloroplastic isozyme
1.27
glutathione
-
isoform DHAR1, at pH 7.8 and 25°C
1.27
glutathione
-
pH and temperature not specified in the publication, cytosolic isozyme DHAR1
1.344
glutathione
-
isoform DHAR2, at pH 6.5 and 25°C
1.41
glutathione
pH and temperature not specified in the publication, cytosolic isozyme
1.466
glutathione
-
isoform DHAR1, at pH 6.5 and 25°C
1.63
glutathione
pH not specified in the publication, temperature not specified in the publication
1.71
glutathione
mutant D72A, pH not specified in the publication, temperature not specified in the publication
2.22
glutathione
-
isoform DHAR2, at pH 7.8 and 25°C
2.22
glutathione
-
pH and temperature not specified in the publication, plastidic isozyme DHAR2
2.255
glutathione
-
isoform DHAR2, at pH 6.5 and 25°C
2.28
glutathione
wild-type, pH not specified in the publication, temperature not specified in the publication
2.28
glutathione
pH and temperature not specified in the publication, cytosolic isozyme DHAR2
2.38
glutathione
pH and temperature not specified in the publication, cytosolic isozyme
2.47
glutathione
pH not specified in the publication, temperature not specified in the publication
2.47
glutathione
pH and temperature not specified in the publication, cytosolic isozyme DHAR3
2.5
glutathione
pH and temperature not specified in the publication, cytosolic isozyme
2.507
glutathione
-
isoform DHAR2, at pH 6.5 and 25°C
2.705
glutathione
-
isoform DHAR1, at pH 6.5 and 25°C
2.883
glutathione
-
isoform DHAR4, at pH 6.5 and 25°C
3.01
glutathione
mutant K8A, pH not specified in the publication, temperature not specified in the publication
3.26
glutathione
mutant S73A, pH not specified in the publication, temperature not specified in the publication
3.7 - 5
glutathione
pH not specified in the publication, temperature not specified in the publication
3.7 - 5
glutathione
pH and temperature not specified in the publication, chloroplastic isozyme DHAR1
4.18
glutathione
mutant with C-terminal residues FGLC deleted, pH 6.9, 30°C
4.35
glutathione
pH and temperature not specified in the publication, cytosolic isozyme
4.82
glutathione
wild-type, pH 6.9, 30°C
5.97
glutathione
mutant containing six cysteine-to-serine mutations, with C-terminal residues FGLC deleted, pH 6.9, 30°C
7.8
glutathione
mutant containing six cysteine-to-serine mutations with the C-terminal cysteine residue deleted, pH 6.9, 30°C
11.4
glutathione
allelic variant D142 of GSTO2-2
11.8
glutathione
allelic variant N142 of GSTO2-2
12.71
glutathione
pH 6.3, temperature not specified in the publication
0.69
GSH
-
mutant enzyme C23S
0.95
GSH
-
mutant enzyme C9S
1.1
GSH
-
wild-type enzyme
1.1
GSH
-
enzyme form DHAR-a and recombinant enzyme form DHAR-a
1.1
GSH
-
mutant enzyme C9S/C26S
2.5
GSH
-
enzyme form DHAR-b
3.7
GSH
-
glutathione-dependent dehydroascorbate reductase activity of thioltransferase (glutaredoxin)
3.7
GSH
-
pH 6.8, in presence of 0.5 mM dehydroascorbate
4.32
GSH
-
pH 6.3, in presence of 0.3 mM dehydroascorbate
5.2
GSH
-
glutathione-dependent dehydroascorbate reductase activity of thioltransferase (glutaredoxin), C25S mutant
additional information
additional information
AtDHAR2 is the first DHAR reported to behave as an allosteric enzyme, and its kcat/K0.5 for DHA is substantially higher than for GSH, suggesting that it has a considerably higher substrate specificity for DHA
-
additional information
additional information
AtDHAR2 is the first DHAR reported to behave as an allosteric enzyme, and its kcat/K0.5 for DHA is substantially higher than for GSH, suggesting that it has a considerably higher substrate specificity for DHA
-
additional information
additional information
AtDHAR2 is the first DHAR reported to behave as an allosteric enzyme, and its kcat/K0.5 for DHA is substantially higher than for GSH, suggesting that it has a considerably higher substrate specificity for DHA
-
additional information
additional information
chloroplastic DHAR displays similar values to the cytosolic isoform, despite having somewhat higher affinity for GSH
-
additional information
additional information
GSH and DHA reduction follows a Michaelis-Menten kinetic
-
additional information
additional information
-
GSH and DHA reduction follows a Michaelis-Menten kinetic
-
additional information
additional information
in Arabidopsis, the cytosolic DHAR1 exhibits higher affinity for DHA than the chloroplastic DHAR3, while their affinities for GSH are approximately the same
-
additional information
additional information
in Arabidopsis, the cytosolic DHAR1 exhibits higher affinity for DHA than the chloroplastic DHAR3, while their affinities for GSH are approximately the same
-
additional information
additional information
in Arabidopsis, the cytosolic DHAR1 exhibits higher affinity for DHA than the chloroplastic DHAR3, while their affinities for GSH are approximately the same
-
additional information
additional information
-
the chloroplastic DHAR of kiwifruit shows higher affinity for DHA than the cytosolic DHAR, while the affinity for GSH of the cytosolic isoform is higher than that of the chloroplastic isoform
-
additional information
additional information
the chloroplastic DHAR of kiwifruit shows higher affinity for DHA than the cytosolic DHAR, while the affinity for GSH of the cytosolic isoform is higher than that of the chloroplastic isoform
-
additional information
additional information
the chloroplastic DHAR of kiwifruit shows higher affinity for DHA than the cytosolic DHAR, while the affinity for GSH of the cytosolic isoform is higher than that of the chloroplastic isoform
-
additional information
additional information
the chloroplastic DHAR of kiwifruit shows higher affinity for DHA than the cytosolic DHAR, while the affinity for GSH of the cytosolic isoform is higher than that of the chloroplastic isoform
-
additional information
additional information
the DHA reaction follows Michaelis-Menten kinetics
-
additional information
additional information
-
the DHA reaction follows Michaelis-Menten kinetics
-