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1.8.5.1: glutathione dehydrogenase (ascorbate)

This is an abbreviated version!
For detailed information about glutathione dehydrogenase (ascorbate), go to the full flat file.

Word Map on EC 1.8.5.1

Reaction

2 glutathione +

dehydroascorbate
=
glutathione disulfide
 +
ascorbate

Synonyms

At1g19570, At1g75270, At5g16710, AtDHAR, AtDHAR1, AtDHAR2, AtDHAR3, CrDHAR1, DasA reductase, dehydroascorbate reductase, dehydroascorbate reductase 2, dehydroascorbic acid reductase, dehydroascorbic reductase, dehydrogenase, glutathione (ascorbate), DHA reductase, DHA-R, DHAR, DHAR1, DHAR2, DHAR3, DHAR3a, DHAR4, DHAR5, dioscorin, GDOR, glutathione dehydroascorbate reductase, glutathione-dependent dehydroascorate reductase, glutathione:dehydroascorbic acid oxidoreductase, GRX1, GRX2, GSH-DHAR, GSH:DHA-oxidoreductase, GSTO, GSTO1, GSTO1-1, GSTO2, GSTO2-2, LcDHAR, metallothionein-1, metallothionein-2, More, MT-I, MT-II, nlGSTO, OsDHAR, OsDHAR1, PbDHAR, PgDHAR1, PtrDHAR1, PtrDHAR2, PtrDHAR3A, PtrDHAR3B, SPD1

ECTree

     1 Oxidoreductases
         1.8 Acting on a sulfur group of donors
             1.8.5 With a quinone or similar compound as acceptor
                1.8.5.1 glutathione dehydrogenase (ascorbate)

Cloned

Cloned on EC 1.8.5.1 - glutathione dehydrogenase (ascorbate)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a fusion DHAR expressed in an Escherichia coli
enzyme form DHAR-a, expression in Escherichia coli
-
expressed in Arabidopsis thaliana
-
expressed in Arabidopsis thaliana ecotype Columbia
-
expressed in Escherichia coli
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) cells, Arabidopsis thaliana, and Nicotiana benthamiana
-
expressed in Escherichia coli C41(DE3) cells
expressed in Escherichia coli NiCo21 (DE3) cells
expressed in Escherichia coli NiCo21(DE3) cells
expressed in Escherichia coli Rossetta pLysS cells
expressed in Nicotiana tabacum
expressed in Solanum tuberosum
-
expression in Escherichia coli
gene AtDHAR2, recombinant expression in tobacco leaves results in increased aluminum tolerance in roots
gene DAHR, DHAR overexpression in tobacco plants
-
gene DHAR, DHAR overexpression in maize plants
gene DHAR1, recombinant expression of wild-type and mutant enzymes in Escherichia coli
gene encoding DHAr, DNA and amino acid sequence determination and analysis, phylogenetic tree
gene gsto1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
isozyme DHAR1, recombinant expression in Arabidopsis thaliana leaves resulting in increased salt and drought tolerance
isozyme DHAR1, recombinant expression in Arabidopsis thaliana leaves resulting in increased salt tolerance. Recombinant expression in Zea mays kernels results in improved nutrition value of grain. Recombinant expression in tobacco leaves recults in decreased GSH/GSSG and increased salt and chilling tolerance. Recombinant overexpression in rice plants results in increased grain yield and biomass, as well as in increased paraquat and salt tolerance
isozyme DHAR1, recombinant overexpression in Arabidopsis thaliana leaves resulting in increased total glutathione content and GSH/GSSG, as well as in increased tolerance to paraquat, high light, and high temperature, Recombinant expression of AtDHAr1 in Solanum tuberosum results in increased tolerance to paraquat, drought, and salt of the potato plants
overexpressed in Escherichia coli BL21 (DE3) strain using the pET-28a(+) expression vector
-
overexpression in transgenic Nicotiana tabacum plants
-
overexpression of cytosolic and plastidic isozymes in transgenic potato plants
-
overproduced in Escherichia coli
production of a triple mutant lacking all three DHARs, dhar1, dhar2, and dhar3, are crossed with each other, resulting in the generation of double mutants dhar1/dhar2, dhar1/dhar3, and dhar2/dhar3. Among these double mutants, dhar1/dhar3 and dhar2/dhar3 are further crossed with each other to generate the triple-knockout mutant dhar1/dhar2/dhar3 (termed DELTAdhar), which is isolated from the F2 population by using PCR-based genotyping
recombinant overexpression of DHAR isozymes in potato plants does not result in different phenotypes. Recombinant expression of the isozymes in Solanum lycopersicum results in no increase in ascorbate content in fruit expressing a chloroplast-localized DHAR, but increased paraquat and salt tolerance
recombinant protein is overexpressed in Escherichia coli
the coding region is expressed as a His-tagged fusion protein in Escherichia coli
-