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1.8.3.7: formylglycine-generating enzyme

This is an abbreviated version!
For detailed information about formylglycine-generating enzyme, go to the full flat file.

Word Map on EC 1.8.3.7

Reaction

a [sulfatase]-L-cysteine
+
O2
 + 2 a thiol =
a [sulfatase]-3-oxo-L-alanine
 +
hydrogen sulfide
 +
a disulfide
 +
H2O

Synonyms

AtsB, C-alpha-formylglycine-generating enzyme 1, Calpha-formylglycine-generating enzyme, Calpha-formylglycine-generating enzyme 1, FGE, FGly-generating enzyme, formylglycine generating enzyme, sulfatase-modifying factor 1, SUMF1

ECTree

     1 Oxidoreductases
         1.8 Acting on a sulfur group of donors
             1.8.3 With oxygen as acceptor
                1.8.3.7 formylglycine-generating enzyme

Engineering

Engineering on EC 1.8.3.7 - formylglycine-generating enzyme

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A177P
A279V
A348P
C218Y
C336R
C336S
C341S
active site mutant
C346W
the mutant show reduced enzyme activity
E130D
G247R
the mutant show reduced enzyme activity
G263V
the mutant show reduced enzyme activity
N259I
P266L
R224W
R345C
R349Q
R349W
R364C
the mutant show reduced enzyme activity
S155P
S234R
the mutant show reduced enzyme activity
W179S
C263S
C187A
-
the mutation leads to an increase of enzyme activity
C187A/C231A/C284S/C298A
C187A/C231A/Y273F/C284S/C298A
-
the mutations lead to an increase of enzyme activity
C187A/C284S/C298A
-
the mutations lead to an increase of enzyme activity
C269S
almost no activity
C274S
C296S
-
inactive
C298A
-
the in vitro activity is not affected by the mutation
S266A
S290K
W228F
kcat/Km of the mutant enzyme is fold lower than the wild-type value
Y273F
-
the mutation leads to an increase of enzyme activity
S266A
-
kcat/Km of the mutant enzyme is fold lower than the wild-type value
-
W228F
-
kcat/Km of the mutant enzyme is fold lower than the wild-type value
-
C187A/C231A/C284S/C298A
-
ten-fold increased activity compared to wild-type
-