1.8.3.7: formylglycine-generating enzyme

This is an abbreviated version!
For detailed information about formylglycine-generating enzyme, go to the full flat file.

Word Map on EC 1.8.3.7

Reaction

a [sulfatase]-L-cysteine
+
O2
+ 2 a thiol =
a [sulfatase]-3-oxo-L-alanine
+
hydrogen sulfide
+
a disulfide
+
H2O

Synonyms

AtsB, C-alpha-formylglycine-generating enzyme 1, Calpha-formylglycine-generating enzyme, Calpha-formylglycine-generating enzyme 1, FGE, FGly-generating enzyme, formylglycine generating enzyme, sulfatase-modifying factor 1, SUMF1

ECTree

     1 Oxidoreductases
         1.8 Acting on a sulfur group of donors
             1.8.3 With oxygen as acceptor
                1.8.3.7 formylglycine-generating enzyme

Engineering

Engineering on EC 1.8.3.7 - formylglycine-generating enzyme

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A177P
A279V
A348P
C218Y
C336R
C336S
C341S
-
active site mutant
C346W
-
the mutant show reduced enzyme activity
E130D
-
the mutation is associated with multiple sulfatase deficiency
G247R
-
the mutant show reduced enzyme activity
G263V
-
the mutant show reduced enzyme activity
N259I
P266L
R224W
R345C
R349Q
R349W
R364C
-
the mutant show reduced enzyme activity
S155P
S234R
-
the mutant show reduced enzyme activity
W179S
C263S
C187A
-
the mutation leads to an increase of enzyme activity
C187A/C231A/C284S/C298A
C187A/C231A/Y273F/C284S/C298A
-
the mutations lead to an increase of enzyme activity
C187A/C284S/C298A
-
the mutations lead to an increase of enzyme activity
C269S
almost no activity
C274S
C296S
-
inactive
C298A
-
the in vitro activity is not affected by the mutation
S266A
S290K
Y273F
-
the mutation leads to an increase of enzyme activity
C187A/C231A/C284S/C298A
-
ten-fold increased activity compared to wild-type
-