1.8.3.2: thiol oxidase

This is an abbreviated version!
For detailed information about thiol oxidase, go to the full flat file.

Reaction

2 R'C(R)SH +

Synonyms

Ac92, AcMNPV P33, ALR, ALRp, AtSOX, augmenter of liver regeneration, DTT-oxidase, E10R, egg white oxidase, Ero1, Ero1p, ERV/ALR sulfhydryl oxidase, Erv1, Erv1p, Erv2, ERv2p, FAD-linked sulfhydryl oxidase, FAD-linked sulfhydryl oxidase ALR, FAD-sulfhydryl oxidase, flavin adenine dinucleotide-linked sulfhydryl oxidase, flavin-dependent sulfhydryl oxidase, GmQSOX1, GmQSOX2, hepatic regenerative stimulator substance, hepatopoietin, mitochondrial FAD-linked sulfhydryl oxidase ERV1, More, neuroblastoma-derived sulfhydryl oxidase, oxidase, thiol, P33, pB119L, protein pB119L, QSCN6, QSOX, QSOx1, QSOX1b, QSOX2, QSOX3, Quiescin Q6, quiescin Q6 sulfhydryl oxidase, quiescin Q6 sulfhydryl oxidase 1, quiescin Q6/sulfhydryl oxidase, quiescin sulfhydryl oxidase, quiescin sulfhydryl oxidase 1, quiescin sulhydryl oxidase, quiescin-like flavin-dependent sulfhydryl oxidase, Quiescin-sulfhydryl oxidase, quiescin/sulfhydryl oxidase, quiescin/sulfhydryl oxidase 1b, quiescin/sulphydryl oxidase, rQSOX, sfALR, SOX, Sox1, Sox2, SOXN, sulfhydryl oxidase, sulfhydryl oxidase SOx-3, sulfhydryl oxidase, P33, sulphydryl oxidase, thiol oxidase Erv1, thiooxidase

ECTree

     1 Oxidoreductases

         1.8 Acting on a sulfur group of donors

             1.8.3 With oxygen as acceptor

                1.8.3.2 thiol oxidase

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Results	in table
8	Activating Compound
9434	AA Sequence
15	Application
1	CAS Registry Number
61	Cloned(Commentary)
58	Cofactor
12	Crystallization (Commentary)
546	Disease/ Diagnostics
4	Expression
56	General Information
2	General Stability
44	Inhibitors
26	kcat/KM [mM/s]
104	KM Value [mM]
80	Localization
14	Metals/Ions
49	Molecular Weight [Da]
47	Natural Substrates/ Products (Substrates)
152	Organism
1	Pathway
78	PDB ID
22	pH Optimum
2	pH Range
2	pH Stability
17	Posttranslational Modification
65	Protein Variants
32	Purification (Commentary)
29	Reaction
3	Reaction Type
139	Reference
150	Source Tissue
16	Specific Activity [micromol/min/mg]
6	Storage Stability
273	Substrates and Products (Substrate)
73	Subunits
235	Synonyms
1	Systematic Name
14	Temperature Optimum [°C]
28	Temperature Stability [°C]
63	Turnover Number [1/s]

KM Value

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KM Value on EC 1.8.3.2 - thiol oxidase

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

7.9 - 54

2-mercaptoethanol

3 entries

100

2-nitro-5-thiobenzoic acid

Bos taurus

-

-

393051

1.25 - 30

cysteamine

3 entries

1.33 - 13.1

D-Cys

3 entries

0.03 - 52.2

dithiothreitol

16 entries

0.14 - 12.5

DTT

4 entries

0.02 - 41.2

glutathione

12 entries

6.31

Gly-Gly-L-Cys

Bos taurus

-

-

393040

0.09 - 20

GSH

8 entries

0.42 - 10.9

L-Cys

7 entries

1.72

N-acetyl-EAQCGTS

Gallus gallus

-

expressed per thiol basis

393061

1.13 - 3.85

N-acetyl-L-Cys

3 entries

3.3

N-acetylcysteine

Sus scrofa

-

-

393044

0.0001 - 0.77

O2

10 entries

0.16

reduced aldolase

Gallus gallus

-

expressed per thiol basis

393061

-

0.215

reduced insulin A chain

Gallus gallus

-

expressed per thiol basis

393061

-

0.3

reduced insulin B chain

Gallus gallus

-

expressed per thiol basis

393061

-

0.11

reduced lysozyme

Gallus gallus

-

expressed per thiol basis

393061

-

0.33

reduced ovalbumin

Gallus gallus

-

expressed per thiol basis

393061

-

1.25

reduced pyruvate kinase

Gallus gallus

-

expressed per thiol basis

393061

-

0.23

reduced riboflavin-binding protein

Gallus gallus

-

expressed per thiol basis

393061

-

0.0174 - 0.115

Reduced ribonuclease

2 entries

-

0.014 - 0.36

RNAse A

5 entries

-

0.36

rRNaseA

Trypanosoma brucei

Q585M6

pH 7.5, 37°C

724281

-

additional information

additional information

7 entries

-

7.9

2-mercaptoethanol

Aspergillus oryzae

Q2U4P3

pH 7.5, 20°C

724056

9.73

2-mercaptoethanol

Aspergillus oryzae

Q2UA33

pH 7.5, 20°C

724654

54

2-mercaptoethanol

Gallus gallus

-

-

393064

1.25

cysteamine

Bos taurus

-

-

393043, 393045

1.6

cysteamine

Sus scrofa

-

-

393044

30

cysteamine

Bos taurus

-

-

393049, 393051

1.33

D-Cys

Bos taurus

-

-

393049, 393051

1.55

D-Cys

Aspergillus oryzae

Q2UA33

pH 7.5, 20°C

724654

13.1

D-Cys

Aspergillus oryzae

Q2U4P3

pH 7.5, 20°C

724056

0.03

dithiothreitol

Trypanosoma brucei

-

mutant CIPHCII, pH 7.0, 25°C

742867

0.086

dithiothreitol

Trypanosoma brucei

Q585M6

pH 7.5, 37°C

724281

0.15

dithiothreitol

Gallus gallus

-

-

393064

0.17

dithiothreitol

Trypanosoma brucei

-

wild-type enzyme, pH 7.0, 25°C

742867

0.22

dithiothreitol

Trypanosoma brucei

-

mutant CIGPCII, pH 7.0, 25°C

742867

0.51

dithiothreitol

Sus scrofa

-

-

393044

0.7

dithiothreitol

Rattus norvegicus

Q6IUU3

-

657645

1.3

dithiothreitol

Homo sapiens

-

pH 7.5, 25°C, recombinant mutant C15A

658104

1.7

dithiothreitol

Homo sapiens

-

pH 7.5, 25°C, recombinant mutant C15A/C74A/C85A/C124A

658104

1.8

dithiothreitol

Homo sapiens

-

pH 7.5, 25°C, recombinant mutant C124A

658104

2

dithiothreitol

Homo sapiens

-

pH 7.5, 25°C, recombinant mutants C74A/C85A and C15A/C124A

658104

2.1

dithiothreitol

Homo sapiens

-

pH 7.5, 25°C, recombinant wild-type enzyme

658104

2.41

dithiothreitol

Aspergillus oryzae

Q2UA33

pH 7.5, 20°C

724654

4.9

dithiothreitol

Aspergillus oryzae

Q2U4P3

pH 7.5, 20°C

724056

11.3

dithiothreitol

Saccharomyces cerevisiae

-

-

690893

52.2

dithiothreitol

Glycine max

-

recombinant enzyme, pH 7.4, 25°C

742490

0.14

DTT

Gallus gallus

-

native enzyme, pH 7.5

657990

0.2

DTT

Gallus gallus

-

7.5, 25°C

659861

6.9

DTT

Saccharomyces cerevisiae

-

Evr2p

657645

12.5

DTT

Gallus gallus

-

60 kDa enzyme fragment, pH 7.5

657990

0.02

glutathione

Gallus gallus

-

-

657645

0.8

glutathione

Aspergillus tubingensis

-

pH 7.4, 20°C

764376

2.78

glutathione

Aspergillus oryzae

Q2UA33

pH 7.5, 20°C

724654

3 - 3.5

glutathione

Saccharomyces cerevisiae

-

pH 7.0, 30°C, mutant enzyme N34A

765345

3.7

glutathione

Aspergillus oryzae

Q2U4P3

pH 7.5, 20°C

724056

4.4

glutathione

Rattus norvegicus

Q6IUU3

-

657645

4.4

glutathione

Saccharomyces cerevisiae

-

pH 7.0, 30°C, wild-type enzyme

765345

6.7

glutathione

Aspergillus niger

-

pH 5.5, 25°C

723985

12

glutathione

Saccharomyces cerevisiae

-

pH 7.0, 30°C, mutant enzyme S32T

765345

13

glutathione

Saccharomyces cerevisiae

-

pH 7.0, 30°C, mutant enzyme S32A

765345

40.5

glutathione

Saccharomyces cerevisiae

-

pH 7.0, 30°C, mutant enzyme S32A/N34A

765345

41.2

glutathione

Saccharomyces cerevisiae

-

pH 7.0, 30°C, mutant enzyme S32T/N34A

765345

0.09

GSH

Bos taurus

-

-

393054

0.3

GSH

Bos taurus

-

-

393051

0.34

GSH

Bos taurus

-

-

393049

0.5

GSH

Rattus norvegicus

-

-

393057

0.73

GSH

Sus scrofa

-

-

393044

3.16

GSH

Bos taurus

-

-

393045

8.16

GSH

Bos taurus

-

-

393040

20

GSH

Gallus gallus

-

-

393061, 393064

0.42

L-Cys

Sus scrofa

-

-

393044

0.8

L-Cys

Bos taurus

-

-

393049, 393051

0.805

L-Cys

Bos taurus

-

-

393043, 393045

0.9

L-Cys

Aspergillus oryzae

Q2U4P3

pH 7.5, 20°C

724056

0.966

L-Cys

Bos taurus

-

-

393043, 393045

6.11

L-Cys

Aspergillus oryzae

Q2UA33

pH 7.5, 20°C

724654

10.9

L-Cys

Gallus gallus

-

-

393064

1.13

N-acetyl-L-Cys

Bos taurus

-

-

393043, 393045

2.35

N-acetyl-L-Cys

Bos taurus

-

-

393040

3.85

N-acetyl-L-Cys

Bos taurus

-

-

393049, 393051

0.0001

O2

Saccharomyces cerevisiae

-

with 0.3 mM reduced thioredoxin as the substrate of the reductive half-reaction and an initial oxygen concentration of around 0.3 mM such that the reduced thioredoxin would be depleted by 10% during the course of the reaction

670697

0.0004

O2

Saccharomyces cerevisiae

-

in the presence of 12.5 mM dithiothreitol

670697

0.0046

O2

Gallus gallus

-

-

657645

0.018

O2

Saccharomyces cerevisiae

-

25°C, recombinant mutant C159S/C176S, in presence of 10 3.5 mM tris(2-carboxyethyl)phosphine

704550

0.027

O2

Saccharomyces cerevisiae

-

25°C, recombinant wild-type enzyme, in presence of 3.5 mM tris(2-carboxyethyl)phosphine

704550

0.057

O2

Saccharomyces cerevisiae

-

25°C, recombinant wild-type enzyme, in presence of 10 mM DTT

704550

0.062

O2

Saccharomyces cerevisiae

-

25°C, recombinant mutant C30S/C33S, in presence of 10 mM DTT

704550

0.087

O2

Saccharomyces cerevisiae

-

25°C, recombinant mutant C159S/C176S, in presence of 10 mM DTT

704550

0.25

O2

Rattus norvegicus

-

-

393057

0.77

O2

Bos taurus

-

-

393045

0.0174

Reduced ribonuclease

Bos taurus

-

corresponds to a sulfhydryl concentration of 0.14 mM

393043

-

0.115

Reduced ribonuclease

Gallus gallus

-

expressed per thiol basis

393061

-

0.014

RNAse A

Gallus gallus

-

pH 8.1, 25°C

659861

-

0.04

RNAse A

Trypanosoma brucei

-

mutant CIPHCII, pH 7.0, 25°C

742867

-

0.22

RNAse A

Glycine max

-

recombinant enzyme, pH 7.4, 25°C

742490

-

0.32

RNAse A

Trypanosoma brucei

-

mutant CIGPCII, pH 7.0, 25°C

742867

-

0.36

RNAse A

Trypanosoma brucei

-

wild-type enzyme, pH 7.0, 25°C

742867

-

additional information

additional information

Homo sapiens

-

redox potential of wild-type and mutant enzymes

658104

-

additional information

additional information

Gallus gallus

-

the activity with small thiols is dominated by the Km value

657645

-

additional information

additional information

Rattus norvegicus

Q6IUU3

the activity with small thiols is dominated by the Km value

657645

-

additional information

additional information

Saccharomyces cerevisiae

-

oxygen consumption kinetic parameters for the WT and Erv1p mutants, overview

704550

-

additional information

additional information

Homo sapiens

-

binding kinetics of immobilized QSOX to various PrP prion proteins

741563

-

additional information

additional information

Homo sapiens

-

pre-steady state kinetics and stopped-flow measurements of sfALR using DTT to slow down the reaction velocity

741890

-

additional information

additional information

Trypanosoma brucei

-

stopped-flow kinetic measurements

742867

-