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1.8.3.2: thiol oxidase

This is an abbreviated version!
For detailed information about thiol oxidase, go to the full flat file.

Word Map on EC 1.8.3.2

Reaction

2 R'C(R)SH +

O2
=
R'C(R)S-S(R)CR'
 +
H2O2

Synonyms

Ac92, AcMNPV P33, ALR, ALRp, AtSOX, augmenter of liver regeneration, DTT-oxidase, E10R, egg white oxidase, Ero1, Ero1p, ERV/ALR sulfhydryl oxidase, Erv1, Erv1p, Erv2, ERv2p, FAD-linked sulfhydryl oxidase, FAD-linked sulfhydryl oxidase ALR, FAD-sulfhydryl oxidase, flavin adenine dinucleotide-linked sulfhydryl oxidase, flavin-dependent sulfhydryl oxidase, GmQSOX1, GmQSOX2, hepatic regenerative stimulator substance, hepatopoietin, mitochondrial FAD-linked sulfhydryl oxidase ERV1, More, neuroblastoma-derived sulfhydryl oxidase, oxidase, thiol, P33, pB119L, protein pB119L, QSCN6, QSOX, QSOx1, QSOX1b, QSOX2, QSOX3, Quiescin Q6, quiescin Q6 sulfhydryl oxidase, quiescin Q6 sulfhydryl oxidase 1, quiescin Q6/sulfhydryl oxidase, quiescin sulfhydryl oxidase, quiescin sulfhydryl oxidase 1, quiescin sulhydryl oxidase, quiescin-like flavin-dependent sulfhydryl oxidase, Quiescin-sulfhydryl oxidase, quiescin/sulfhydryl oxidase, quiescin/sulfhydryl oxidase 1b, quiescin/sulphydryl oxidase, rQSOX, sfALR, SOX, Sox1, Sox2, SOXN, sulfhydryl oxidase, sulfhydryl oxidase SOx-3, sulfhydryl oxidase, P33, sulphydryl oxidase, thiol oxidase Erv1, thiooxidase

ECTree

     1 Oxidoreductases
         1.8 Acting on a sulfur group of donors
             1.8.3 With oxygen as acceptor
                1.8.3.2 thiol oxidase

Cloned

Cloned on EC 1.8.3.2 - thiol oxidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
CHO-tPA cell-line stably expressing QSOX1-V5 with a C-terminal KDEL sequence
DNA and amino acid sequence determination and analysis expression in human embryonic kidney HEK cells and in rat kangaroo kidney epithelium Pt-K2 cells
DNA and amino acid sequence determination and analysis, gene structure
expressed in Escherichia coli strain B(DE3)pLysS
-
expressed in Escherichia coli strain XL-1
-
expression in Chineses hamster ovary epithelium cells
expression in Escherichia coli
expression in Escherichia coli Rosetta(DE3)pLysS strain
-
expression in Escherichia coli Rosetta-gamiB (DE3) cells
expression in Escherichia coli strain BL21(DE3)
expression in Escherichia coli strains DH5alpha and BL21(DE3) of wild-type enzyme and a His-tagged truncated enzyme form comprising the 15 kDa C-terminus, expression of full-length point mutants
expression in Trichoderma reesei
expression of His6-tagged Alrp in Escherichia coli, expression of GFP-fusion enzyme in cell culture
-
expression of His6-tagged enzyme in Escherichia coli
-
expression of wild-type and mutant enzymes in Escherichia coli strain BC21
gene AtErv1, DNA and amino acid sequence analysis, subcloning in Escherichia coli strain DH5alpha, expression of full length enzyme and 15 kDa C-terminal fragment as His-tagged proteins in Escherichia coli strain BL21, transient expression of the enzyme fused to GFP in protoplasts of Arabidopsis thaliana and of Physcomitrella patens with localization in the mitochondria, no complementation of the enzyme-defective yeast mutant erv-ts and yeast deletion mutant DELTAerv1
gene cpQSOx1, primary gene sequence, DNA and amino acid sequence analysis, stable expression of the wild-type enzyme in MCF-7 cells with intra- and extracellular localization of the recombinant enzyme, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene GmQSOX1, in two slicing variants GmQSOX1a and GmQSOX1b, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression of the two variants without the putative signal peptide as soluble His-tagged proteins in Escherichia coli strain Rosetta-gami B (DE3)
-
gene GmQSOX2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree
-
gene QSOX, DNA and amino acid sequence comparisons and phylogenetic analysis
gene QSOX, isozyme QSOX1 has two splising variants 1a and 1b, DNA and amino acid sequence comparisons and phylogenetic analysis
gene QSOX1, DNA and amino acid sequence comparisons and phylogenetic analysis
gene QSOX1, DNA and amino acid sequence determination and analysis, quantitative real-time PCR enzyme expression analysis, two putative alternative splicing variants might contribute to the differences in expression level between exon 7 and the 3'UTR are QSOX1d and QSOX1e. In QSOX1d the thioredoxin TRX1 domain remains intact, whilst the TRX2 and HRR domains are removed completely, making these isoforms similar to plant QSOXs which lack the second Trx domain but have the same three redox sites. TRX1 is truncated at its C-terminus in QSOX1e and both of the splicing
gene QSOX2, DNA and amino acid sequence comparisons and phylogenetic analysis
gene QSOX3, DNA and amino acid sequence comparisons and phylogenetic analysis
-
gene SOXN, DNA and amino acid sequence determination, gene maps to chromosome 9q34.3, subcloning in Escherichia coli strain DH10B, in vitro-transcription and -translation
genes ERV1 and ERV2, DNA and amino acid sequence determination and analysis, gene structure, phylogenetic analysis
-
genes QSCN6 or QSOX1, and QSOX2, DNA and amino acid sequence determination and analysis, QSCN6 or QSOX1 is located on chromosome 1q25.2, QSOX2 on chromosome 9q34, gene structure
into the pcDNA3.1+ plasmid vector
-
overexpression in MCF-7 cell lines
overexpression of wild-type and mutant liver enzymes in Escherichia coli strain BL21(DE3) with a longer or shorter linker His-tag, overview
-
truncated version of Erv2p, without the N-terminal 34 residues, cloned into a pET24(a+) plasmid carrying kanamycin resistance. Amplified in DH5alpha Escherichia coli strain. Expressed from the Escherichia coli strain BL21(DE3) or BL21(DE3 star)
-