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0.34
cytochrome c
-
mutant enzyme G473D, pH 6.0
0.48
cytochrome c
-
mutant enzyme G473D, pH 8.6
0.53
cytochrome c
-
mutant enzyme G473D, pH 6.5
0.59
cytochrome c
-
mutant enzyme G473D, pH 8.0
0.62
cytochrome c
-
mutant enzyme G473D, pH 7.5
0.78
cytochrome c
-
mutant enzyme G473D, pH 7.0
1.67
cytochrome c
-
mutant enzyme G473W, pH 6.0
1.95
cytochrome c
-
mutant enzyme G473W, pH 8.5
2.94
cytochrome c
-
mutant enzyme G473W, pH 7.0
3.83
cytochrome c
-
mutant enzyme G473A, pH 6.0
12.4
cytochrome c
-
wild type enzyme, pH 6.0
12.8
cytochrome c
-
mutant enzyme G473A, pH 7.0
18.1
cytochrome c
-
wild type enzyme, pH 7.0
25.4
cytochrome c
-
mutant enzyme G473A, pH 8.5
26.9
cytochrome c
-
wild type enzyme, pH 8.5
0.14
sulfite
-
mutant enzyme G473D in 20 mM Tris at pH 6.0
0.14
sulfite
-
mutant enzyme G473D, pH 6.0
0.15
sulfite
-
mutant enzyme A208D in 20 mM Tris at pH 6.0
0.28
sulfite
-
mutant enzyme G473D, pH 6.5
0.42
sulfite
-
mutant enzyme G473D, pH 9.1
0.46
sulfite
-
mutant R472M, 25°C, pH 10.0
0.5
sulfite
-
mutant enzyme G473D, pH 7.0
0.52
sulfite
-
mutant R472Q, 25°C, pH 10.0
0.54
sulfite
-
mutant enzyme G473D in 20 mM Tris at pH 8.5
0.54
sulfite
-
mutant enzyme G473D, pH 8.5
0.57
sulfite
-
mutant enzyme G473D, pH 7.5
0.58
sulfite
-
mutant enzyme G473D, pH 8.0
0.6
sulfite
-
mutant enzyme G473W in 20 mM Tris at pH 6.0
0.6
sulfite
-
mutant enzyme G473W, pH 6.0
0.75
sulfite
-
mutant enzyme A208D in 20 mM Tris at pH 8.5
0.97
sulfite
-
mutant Y343N/R472Q, 25°C, pH 9.5
1.13
sulfite
-
mutant Y343N/R472Q, 25°C, pH 7.0
1.13
sulfite
-
mutant Y343N/R472Q, 25°C, pH 9.0
1.33
sulfite
-
mutant Y343N/R472Q, 25°C, pH 8.0
1.35
sulfite
-
mutant enzyme G473W, pH 9.0
1.35
sulfite
-
mutant Y343N/R472Q, 25°C, pH 6.0
1.42
sulfite
-
mutant Y343N/R472Q, 25°C, pH 8.5
1.44
sulfite
-
mutant Y343F/R472Q, 25°C, pH 6.0
1.7
sulfite
-
mutant R472Q, 25°C, pH 9.5
1.73
sulfite
-
mutant Y343F/R472Q, 25°C, pH 7.0
1.8
sulfite
-
mutant enzyme G473W, pH 7.0
1.8
sulfite
-
mutant R472M, 25°C, pH 9.5
1.9
sulfite
-
mutant Y343N/R472M/V474M, 25°C, pH 6.0
2.05
sulfite
-
mutant Y343F/R472Q, 25°C, pH 8.0
2.06
sulfite
-
25°C, pH 7.0, 100 mM buffer, mutant enzyme Y343F
2.25
sulfite
-
mutant Y343F/R472Q, 25°C, pH 8.5
2.3
sulfite
-
mutant Y343F/R472Q, 25°C, pH 9.0
2.48
sulfite
-
mutant enzyme G473W in 20 mM Tris at pH 8.5
2.48
sulfite
-
mutant enzyme G473W, pH 8.5
2.52
sulfite
mutant Y322N/R450M, 25°C, pH 8.5
2.8
sulfite
-
mutant Y343N/R472M/V474M, 25°C, pH 10.0
2.96
sulfite
-
mutant Y343F/R472Q, 25°C, pH 10.0
3 - 6
sulfite
-
mutant Y83F, pH 8.0, 25°C
3.11
sulfite
-
25°C, pH 6.0, 20 mM buffer, mutant enzyme Y343F
3.17
sulfite
-
mutant Y343N, 25°C, pH 6.0
3.26
sulfite
-
25°C, pH 7.5, 100 mM buffer, mutant enzyme Y343F
3.4
sulfite
-
mutant Y343N/R472M/V474M, 25°C, pH 7.0
3.45
sulfite
-
mutant R472M, 25°C, pH 9.0
3.5
sulfite
-
mutant R472M, 25°C, pH 8.0
3.58
sulfite
-
mutant Y343N/R472M/V474M, 25°C, pH 8.0
3.6
sulfite
-
mutant R472M, 25°C, pH 7.0
3.8
sulfite
-
mutant R472M, 25°C, pH 8.5
3.9
sulfite
-
mutant Y343N/R472M/V474M, 25°C, pH 8.5
4.14
sulfite
-
25°C, pH 6.5, 20 mM buffer, mutant enzyme Y343F
4.15
sulfite
-
mutant enzyme G473A in 20 mM Tris at pH 6.0
4.15
sulfite
-
mutant enzyme G473A, pH 6.0
4.24
sulfite
-
mutant R472Q, 25°C, pH 9.0
4.38
sulfite
-
mutant Y343N, 25°C, pH 10.0
4.59
sulfite
-
25°C, pH 7.0, 20 mM buffer, mutant enzyme Y343F
4.6
sulfite
-
mutant R472Q, 25°C, pH 8.5
4.83
sulfite
mutant Y322N/R450M, 25°C, pH 7.0
4.9
sulfite
-
mutant Y343F/R472Q, 25°C, pH 9.5
5
sulfite
-
mutant R472M, 25°C, pH 6.0
5.23
sulfite
-
mutant Y343N/R472M/V474M, 25°C, pH 9.5
5.6
sulfite
-
mutant Y343N/R472M/V474M, 25°C, pH 9.0
5.7
sulfite
-
mutant R472D, pH 6.5, 25°C
5.8
sulfite
-
mutant R472Q, 25°C, pH 7.0
5.96
sulfite
-
mutant V474M, 25°C, pH 6.0
6.35
sulfite
-
25°C, pH 7.5, 20 mM buffer, mutant enzyme Y343F
7
sulfite
-
mutant Y83A, pH 8.0, 25°C
7.17
sulfite
-
25°C, pH 8.0, 100 mM buffer, mutant enzyme Y343F
8.1
sulfite
-
mutant Y343N, 25°C, pH 9.5
8.2
sulfite
-
mutant R472Q, 25°C, pH 6.0
8.21
sulfite
-
25°C, pH 10.0, 100 mM buffer, mutant enzyme Y343F
8.51
sulfite
-
25°C, pH 10.0, 20 mM buffer, mutant enzyme Y343F
8.59
sulfite
-
25°C, pH 8.0, 20 mM buffer, mutant enzyme Y343F
8.72
sulfite
-
25°C, pH 8.25, 100 mM buffer, mutant enzyme Y343F
9.08
sulfite
-
25°C, pH 8.25, 20 mM buffer, mutant enzyme Y343F
9.2
sulfite
-
25°C, pH 8.5, 100 mM buffer, mutant enzyme Y343F
9.24
sulfite
-
25°C, pH 8.5, 20 mM buffer, mutant enzyme Y343F
9.3
sulfite
-
mutant R472Q, 25°C, pH 8.0
9.4
sulfite
with ferricanide, pH 6.0, 25°C, recombinant mutant C242S/C253S/C260S/C451S
9.47
sulfite
-
25°C, pH 9.0, 20 mM buffer, mutant enzyme Y343F
9.63
sulfite
-
25°C, pH 9.5, 20 mM buffer, mutant enzyme Y343F
9.92
sulfite
-
25°C, pH 9.75, 20 mM buffer, mutant enzyme Y343F
9.99
sulfite
-
25°C, pH 9.0, 100 mM buffer, mutant enzyme Y343F
10.5
sulfite
-
25°C, pH 9.5, 100 mM buffer, mutant enzyme Y343F
11.4
sulfite
-
mutant V474M, 25°C, pH 7.0
12.1
sulfite
-
25°C, pH 7.0, 100 mM buffer, wild-type enzyme
12.4
sulfite
-
mutant V474M, 25°C, pH 10.0
12.8
sulfite
-
mutant Y343N, 25°C, pH 7.0
13
sulfite
-
mutant F79A, pH 8.0, 25°C
13
sulfite
-
wild-type, 25°C, pH 10.0
13.2
sulfite
-
25°C, pH 6.0, 20 mM buffer, wild-type enzyme
13.2
sulfite
-
wild type enzyme in 20 mM Tris at pH 6.0
13.2
sulfite
-
wild type enzyme, pH 6.0
13.2
sulfite
-
wild-type, 25°C, pH 6.0
13.75
sulfite
-
mutant Y343N, 25°C, pH 8.0
13.9
sulfite
with ferricanide, pH 6.0, 25°C, recombinant wild-type enzyme
14.1
sulfite
-
mutant R472D, pH 7.6, 25°C
15.1
sulfite
-
mutant enzyme G473A, pH 10.0
15.5
sulfite
-
mutant Y343N, 25°C, pH 9.0
15.8
sulfite
-
mutant V474M, 25°C, pH 8.5
15.9
sulfite
-
mutant enzyme G473A, pH 7.0
16
sulfite
-
mutant F57A, pH 8.0, 25°C
16.2
sulfite
with ferricanide, pH 7.1, 25°C, recombinant wild-type enzyme
16.9
sulfite
-
mutant Y343N, 25°C, pH 8.5
17.1
sulfite
-
mutant V474M, 25°C, pH 9.5
17.2
sulfite
-
25°C, pH 7.5, 100 mM buffer, wild-type enzyme
17.7
sulfite
-
25°C, pH 6.5, 20 mM buffer, wild-type enzyme
17.7
sulfite
-
wild type enzyme, pH 6.5
17.8
sulfite
-
mutant V474M, 25°C, pH 8.0
18.5
sulfite
-
mutant R472K, pH 7.6, 25°C
19
sulfite
-
mutant F57Y, pH 8.0, 25°C
19
sulfite
-
mutant H90F, pH 8.0, 25°C
19.6
sulfite
-
mutant V474M, 25°C, pH 9.0
19.8
sulfite
with ferricanide, pH 7.1, 25°C, recombinant mutant C242S/C253S/C260S/C451S
20.8
sulfite
with ferricanide, pH 7.1, 25°C, recombinant wild-type enzyme
21.6
sulfite
-
mutant enzyme G473A, pH 7.4
23
sulfite
-
mutant D342K, pH 7.6, 25°C
23.6
sulfite
-
25°C, pH 8.75, 20 mM buffer, wild-type enzyme
24.2
sulfite
-
25°C, pH 7.0, 20 mM buffer, wild-type enzyme
24.2
sulfite
-
wild-type, 25°C, pH 7.0
24.2
sulfite
-
wild type enzyme, pH 7.0
24.6
sulfite
-
25°C, pH 9.5, 100 mM buffer, wild-type enzyme
24.7
sulfite
-
wild type enzyme, pH 7.5
24.7
sulfite
-
25°C, pH 7.5, 20 mM buffer, wild-type enzyme
24.8
sulfite
-
25°C, pH 8.25, 20 mM buffer, wild-type enzyme
25
sulfite
-
25°C, pH 8.0, 100 mM buffer, wild-type enzyme
25.7
sulfite
-
25°C, pH 9.0, 20 mM buffer, wild-type enzyme
25.7
sulfite
-
wild type enzyme, pH 9.0
25.7
sulfite
-
wild-type, 25°C, pH 9.0
25.8
sulfite
with ferricanide, pH 8.4, 25°C, recombinant mutant C242S/C253S/C260S/C451S
25.9
sulfite
-
25°C, pH 8.0, 20 mM buffer, wild-type enzyme
25.9
sulfite
-
wild type enzyme, pH 8.0
25.9
sulfite
-
wild-type, 25°C, pH 8.0
26.1
sulfite
with ferricanide, pH 6.0, 25°C, recombinant selenomethionine-labeled mutant C242S/C253S/C260S/C451S
26.2
sulfite
-
mutant enzyme G473A, pH 8.0
26.3
sulfite
-
25°C, pH 9.5, 20 mM buffer, wild-type enzyme
26.3
sulfite
-
wild type enzyme, pH 9.5
26.3
sulfite
-
wild-type, 25°C, pH 9.5
26.6
sulfite
-
mutant R472Q, pH 7.6, 25°C
26.9
sulfite
-
wild type enzyme, pH 8.5
26.9
sulfite
-
wild-type, pH 8.0, 25°C
26.9
sulfite
-
25°C, pH 8.5, 100 mM buffer, wild-type enzyme
26.9
sulfite
-
25°C, pH 8.5, 20 mM buffer, wild-type enzyme
26.9
sulfite
-
wild type enzyme in 20 mM Tris at pH 8.5
26.9
sulfite
-
wild-type, 25°C, pH 8.5
27
sulfite
-
wild-type, pH 8.0, 25°C
27
sulfite
-
25°C, pH 8.25, 100 mM buffer, wild-type enzyme
27
sulfite
-
mutant R472M, pH 7.6, 25°C
27.3
sulfite
with ferricanide, pH 8.9, 25°C, recombinant wild-type enzyme
28.1
sulfite
-
25°C, pH 9.0, 100 mM buffer, wild-type enzyme
28.4
sulfite
-
mutant enzyme G473A in 20 mM Tris at pH 8.5
28.4
sulfite
-
mutant enzyme G473A, pH 8.5
31.8
sulfite
with ferricanide, pH 8.9, 25°C, recombinant selenomethionine-labeled mutant C242S/C253S/C260S/C451S
31.9
sulfite
-
mutant enzyme G473A, pH 9.1
32.4
sulfite
with ferricanide, pH 8.4, 25°C, recombinant wild-type enzyme
36.1
sulfite
wild-type, 25°C, pH 7.0
36.2
sulfite
with ferricanide, pH 8.9, 25°C, recombinant mutant C242S/C253S/C260S/C451S
37.7
sulfite
with ferricanide, pH 7.1, 25°C, recombinant selenomethionine-labeled mutant C242S/C253S/C260S/C451S
41.2
sulfite
with ferricanide, pH 8.4, 25°C, recombinant selenomethionine-labeled mutant C242S/C253S/C260S/C451S
42
sulfite
-
mutant H90Y, pH 8.0, 25°C
46.6
sulfite
with ferricanide, pH 7.1, 25°C, recombinant selenomethionine-labeled mutant C242S/C253S/C260S/C451S
47
sulfite
-
mutant R472D/D342K, pH 7.6, 25°C
73
sulfite
wild-type, 25°C, pH 8.5