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1.8.1.9: thioredoxin-disulfide reductase

This is an abbreviated version!
For detailed information about thioredoxin-disulfide reductase, go to the full flat file.

Word Map on EC 1.8.1.9

Reaction

thioredoxin
+
NADP+
=
thioredoxin disulfide
 +
NADPH
 +
H+

Synonyms

all0737, ApTR, At2g41680, AtNTRA, AtNTRB, bacillithiol disulfide reductase, bacillithiol-disulfide reductase, BBOV_I002190, Bdr, BSSB reductase, CeTR2, DmTR, DmTrxR, DmTrxR-1, EC 1.6.4.5, EhTRXR, ferredoxin:thioredoxin reductase, FTR, general stress protein 35, GSP35, HCOI_01258400, hemolysate thioredoxin reductase, hemolysate TR, hTrxR, HvNTR1, HvNTR2, L-TR, L-TrxR, More, MtNTRC, mTR3, multifunctional thioredoxin-glutathione reductase, NAD(P)H:paraquat oxidoreductase, NADP-dependent thioredoxin reductase, NADP-linked thioredoxin reductase, NADP-thioredoxin reductase, NADP-thioredoxin reductase C, NADPH thioredoxin reductase, NADPH thioredoxin reductase C, NADPH-dependent thioredoxin reductase, NADPH-dependent thioredoxin reductase 2, NADPH-dependent thioredoxin reductase C, NADPH-dependent thioredoxin reductase I, NADPH-dependent thioredoxin reductase-1, NADPH-dependent TRX reductase, NADPH-thioredoxin reductase, NADPH-thioredoxin reductase C, NADPH-Trx reductase, NADPH2:oxidized thioredoxin oxidoreductase, NAPDH-dependent BSSB reductase, NTR, Ntr1, NTR2, Ntr3, NTRA, NTRAB, NtrB, NTRC, PH0178, PH1426, PhRP, PhTrxR, protein-disulfide oxidoreductase, reductase, thioredoxin, SEP1, taTrxR, TGR, thioredoxin glutathione reductase, thioredoxin h reductase, thioredoxin reductase, thioredoxin reductase (NADPH), thioredoxin reductase 1, thioredoxin reductase 2, thioredoxin reductase-1, thioredoxin reductase1, TON_1603, TR, TR1, TR2, TR3, TRase, TrR, Trr1, Trr1p, TRR2, Trx1, Trx3, TrxB, TrxB1, TrxB2, TrxR, TrxR-1(cyto), TrxR-1(mito), TrxR1, TrxR2, TrxR3, TrxRB3, TRXRD, TrxRh1, TrxRh2, TrxT, Txnrd1, TXNRD1-v3, Txnrd2, TXNRD3, TxrR-1, YpdA

ECTree

     1 Oxidoreductases
         1.8 Acting on a sulfur group of donors
             1.8.1 With NAD+ or NADP+ as acceptor
                1.8.1.9 thioredoxin-disulfide reductase

Purification

Purification on EC 1.8.1.9 - thioredoxin-disulfide reductase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2',5'-ADP Sepharose column chromatography and phenylarsine oxide Sepharose column chromatography
2',5'-ADP Sepharose column chromatography and phenylarsine oxide-Sepharose column chroamtography
2',5'-ADP Sepharose column chromatography, PAO Sepharose column chromatography, and Superdex 200 gel filtration
-
2',5'-ADP-agarose affinity chromatography and Superose size exclusion chromatography
-
affinity chromatography
ammonium sulfate precipitation and Sephadex G-200 gel filtration
-
ammonium sulfate precipitation, 2'5'-ADP Sepharose 4B affinity column chromatography, and Sephadex G-100 gel filtration
-
ammonium sulfate precipitation, DEAE-Sephacel gel filtration, 2',5'-ADP-Sepharose 4B affinity chromatography, and omega-aminohexyl-Sepharose 4B column chromatography
-
cation-exchange cromatography
-
chitin resin column chromatography and DEAE anion exchange column chromatography
chitin-agarose column gel filtration and S200 column size-exclusion chromatography
-
DE52 column chromatography
-
DEAE column column chromatography, 2',5'-ADP Sepharose 4B column chromatography, phenyl Sepharose column chromatography, and Ni-NTA agarose column chromatography
DEAE-Sepharose column chromatography, hydroxyapatite column chromatography, phenyl-Sepharose column chromatography, and Q-Sepharose column chromatography
-
DEAE-Toyopearl column chromatography and Diol-150 gel filtration
HeLa cells, partially
-
heparin affinity depends on the selenium content, binding can be induced by reduction of the enzyme with NADPH or tris-(2-carboxyethyl)phosphine
-
HisBind HiTrap metal-affinity column chromatography, 2',5'-ADP-Sepharose column chromatography, and CNBr-activated Sepharose column chromatography
HisTrap HP affinity column chromatography, gel filtration
mitochondrial isoform
-
Ni Sepharose 6 Fast Flow column chromatography
Thermosynechococcus vestitus
-
Ni-NTA affinity column chromatography
-
Ni-NTA affinity column chromatography, gel filtration
Ni-NTA column chromatography
Ni-NTA His Bind resin column chromatography
-
Ni-NTA resin affinity chromatography
-
Ni-Sepharose column chromatography
Ni2+ affinity column chromatography
Ni2+ affinity column chromatography and Superose 6 gel filtration
Ni2+-affinity column chromatography
-
Ni2+-chelating Sepharose column chromatography and Superdex 75 column gel filtration
nickel-affinity chromatography
-
partial
-
POROS MC50 metal-chelation column chromatography
-
purification from liver
-
Q-Sepharose column chromatography
-
recombinant enzyme with an amino-terminal hexa-His-tag, extrasequence does not affect enzymatic activity, one-step Ni-NTA affinity column chromatography
recombinant enzyme-thioredoxin subunit complex C135-C32 and mutant S135-S32
-
recombinant from Escherichia coli
-
recombinant His-tagged enzyme from Escherichia coli strain BL21
recombinant His-tagged enzyme from Escherichia coli strain BL21, native Bdr by BrxC-dependent affinity chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli
-
recombinant wild-type and mutants from Escherichia coli
-
recombinant wild-type cytosolic and mitochondrial isoforms and mutants from Escherichia coli
-
recombinant wild-type fusion protein and truncated mutant
-
recombinant, wild-type and mutant C53S
Sepharose affinity column chromatography
-
TALONTM column chromatography and Sepharose affinity column chromatography
-
wild-type and mutant enzymes
wild-type from overexpressing cell line, mutants
-
wild-type from plasmid
-