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0.075 - 42
5,5'-dithio-bis(2-nitrobenzoic acid)
0.17 - 748.3
5,5'-dithiobis(2-nitrobenzoic acid)
7343 - 10620
5-hydroxy-1,4-naphthoquinone
282 - 12300
Arabidopsis thaliana thioredoxin 3
-
80
Babesia bovis thioredoxin
pH 8.0, 30°C
-
470
ebsulfur
-
pH 7.5, temperature not specified in the publication
60.5
ebsulfur disulfide
-
pH 7.5, temperature not specified in the publication
10 - 63.1
Escherichia coli thioredoxin
-
9840
Hordeum vulgare thioredoxin 1
wild-type, pH 7.5, temperature not specified in the publication
-
140 - 6800
Hordeum vulgare thioredoxin 2
-
1630 - 10200
Hordeum vulgare thioredoxin 2 mutant E86A
-
5490
Hordeum vulgare thioredoxin 2 mutant G57P
wild-type, pH 7.5, temperature not specified in the publication
-
1030
Hordeum vulgare thioredoxin 2 mutant I51G
wild-type, pH 7.5, temperature not specified in the publication
-
667
lipoic acid
-
isoform TrxR1, in 50 mM Tris-HCl, 1 mM EDTA, pH 8.0, at 37°C
34000
thioredoxin 1
-
wild type enzyme, pH and temperature not specified in the publication
52000 - 73000
thioredoxin 2
-
31000
thioredoxin 3
-
wild type enzyme, pH and temperature not specified in the publication
-
600 - 880
thioredoxin 41
-
890 - 950
thioredoxin 8
-
13.7 - 65940
thioredoxin disulfide
0.075
5,5'-dithio-bis(2-nitrobenzoic acid)
pH 8.0, 30°C
0.1
5,5'-dithio-bis(2-nitrobenzoic acid)
truncated mutant, pH 7.2, temperature not specified in the publication
0.38
5,5'-dithio-bis(2-nitrobenzoic acid)
pH 7.2, temperature not specified in the publication
4.33
5,5'-dithio-bis(2-nitrobenzoic acid)
pH 7.2, temperature not specified in the publication
42
5,5'-dithio-bis(2-nitrobenzoic acid)
-
pH 7.8, 37°C
0.17
5,5'-dithiobis(2-nitrobenzoic acid)
-
with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C
0.22
5,5'-dithiobis(2-nitrobenzoic acid)
-
wild type enzyme, in 50 mM potassium phosphate at pH 7.0
0.23
5,5'-dithiobis(2-nitrobenzoic acid)
-
with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C
4.78
5,5'-dithiobis(2-nitrobenzoic acid)
-
truncated thioredoxin reductase missing its final eight amino acids, in 50 mM potassium phosphate at pH 7.0
5.27
5,5'-dithiobis(2-nitrobenzoic acid)
-
truncated thioredoxin reductase missing its final eight amino acids, in 50 mM potassium phosphate at pH 7.0
5.45
5,5'-dithiobis(2-nitrobenzoic acid)
-
wild type enzyme, in 50 mM potassium phosphate at pH 7.0
21.2
5,5'-dithiobis(2-nitrobenzoic acid)
-
isoform TrxR2, in 50 mM Tris-HCl, 1 mM EDTA, pH 7.0, at 37°C
45.33
5,5'-dithiobis(2-nitrobenzoic acid)
-
wild type enzyme, in 50 mM potassium phosphate at pH 7.0
58.33
5,5'-dithiobis(2-nitrobenzoic acid)
-
truncated thioredoxin reductase missing its final eight amino acids, in 50 mM potassium phosphate at pH 7.0
95
5,5'-dithiobis(2-nitrobenzoic acid)
-
with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication
178
5,5'-dithiobis(2-nitrobenzoic acid)
-
isoform TrxR1, in 50 mM Tris-HCl, 1 mM EDTA, pH 7.0, at 37°C
183.3
5,5'-dithiobis(2-nitrobenzoic acid)
purified, recombinant, tetrameric enzyme
333.5
5,5'-dithiobis(2-nitrobenzoic acid)
-
wild type enzyme, 10 mM potassium phosphate buffer (pH 7.0) containing 10 mM EDTA, at 25°C
495
5,5'-dithiobis(2-nitrobenzoic acid)
purified, recombinant, dimeric enzyme
513.3
5,5'-dithiobis(2-nitrobenzoic acid)
mutant enzyme Y116I, in TE buffer, at 20°C
526.7
5,5'-dithiobis(2-nitrobenzoic acid)
mutant enzyme Y116T, in TE buffer, at 20°C
748.3
5,5'-dithiobis(2-nitrobenzoic acid)
wild type enzyme, in TE buffer, at 20°C
7343
5-hydroxy-1,4-naphthoquinone
mutant enzyme Y116I, in TE buffer, at 20°C
7557
5-hydroxy-1,4-naphthoquinone
mutant enzyme Y116T, in TE buffer, at 20°C
10620
5-hydroxy-1,4-naphthoquinone
wild type enzyme, in TE buffer, at 20°C
282
Arabidopsis thaliana thioredoxin 3
mutant Delta42-47, pH 7.5, temperature not specified in the publication
-
361
Arabidopsis thaliana thioredoxin 3
mutant W42A/M43A, pH 7.5, temperature not specified in the publication
-
1050
Arabidopsis thaliana thioredoxin 3
mutant M43A, pH 7.5, temperature not specified in the publication
-
2550
Arabidopsis thaliana thioredoxin 3
mutant N45A/D46A, pH 7.5, temperature not specified in the publication
-
4240
Arabidopsis thaliana thioredoxin 3
mutant W42A, pH 7.5, temperature not specified in the publication
-
5340
Arabidopsis thaliana thioredoxin 3
mutant G225R/G226D/P227V, pH 7.5, temperature not specified in the publication
-
6000
Arabidopsis thaliana thioredoxin 3
wild-type, pH 7.5, temperature not specified in the publication
-
7460
Arabidopsis thaliana thioredoxin 3
mutant G225R/G226D, pH 7.5, temperature not specified in the publication
-
9620
Arabidopsis thaliana thioredoxin 3
mutant G222D/A223G/G224E, pH 7.5, temperature not specified in the publication
-
12300
Arabidopsis thaliana thioredoxin 3
pH 7.5, temperature not specified in the publication
-
10
Escherichia coli thioredoxin
pH 8.0, 30°C
-
63.1
Escherichia coli thioredoxin
wild-type, pH 7.5, temperature not specified in the publication
-
140
Hordeum vulgare thioredoxin 2
pH 7.5, temperature not specified in the publication
-
228
Hordeum vulgare thioredoxin 2
mutant W42A, pH 7.5, temperature not specified in the publication
-
713
Hordeum vulgare thioredoxin 2
mutant M43A, pH 7.5, temperature not specified in the publication
-
1490
Hordeum vulgare thioredoxin 2
mutant R140A, pH 7.5, temperature not specified in the publication
-
1710
Hordeum vulgare thioredoxin 2
mutant G225R/G226D/P227V, pH 7.5, temperature not specified in the publication
-
3300
Hordeum vulgare thioredoxin 2
mutant N45A/D46A, pH 7.5, temperature not specified in the publication
-
5410
Hordeum vulgare thioredoxin 2
mutant G222D/A223G/G224E, pH 7.5, temperature not specified in the publication
-
6180
Hordeum vulgare thioredoxin 2
mutant N139A, pH 7.5, temperature not specified in the publication
-
6330
Hordeum vulgare thioredoxin 2
mutant G225R/G226D, pH 7.5, temperature not specified in the publication
-
6800
Hordeum vulgare thioredoxin 2
wild-type, pH 7.5, temperature not specified in the publication
-
1630
Hordeum vulgare thioredoxin 2 mutant E86A
mutant R140A, pH 7.5, temperature not specified in the publication
-
9230
Hordeum vulgare thioredoxin 2 mutant E86A
wild-type, pH 7.5, temperature not specified in the publication
-
10200
Hordeum vulgare thioredoxin 2 mutant E86A
mutant N139A, pH 7.5, temperature not specified in the publication
-
5.6
Lipoamide
mutant enzyme Y116T, in TE buffer, at 20°C
7.5
Lipoamide
mutant enzyme Y116I, in TE buffer, at 20°C
372
Lipoamide
-
isoform TrxR2, in 50 mM Tris-HCl, 1 mM EDTA, pH 8.0, at 37°C
1737
Lipoamide
-
isoform TrxR1, in 50 mM Tris-HCl, 1 mM EDTA, pH 8.0, at 37°C
0.6
NADH
-
in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication
1.1
NADH
-
cosubstrate: 5,5'-dithiobis(2-nitrobenzoic acid), pH and temperature not specified in the publication
2.48
NADH
truncated mutant, pH 7.2, temperature not specified in the publication
84
NADH
-
with 5,5'-dithiobis(2-nitrobenzoicacid) as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C
26.5
NADPH
pH 7.2, temperature not specified in the publication
103.3
NADPH
-
cosubstrate: 5,5'-dithiobis(2-nitrobenzoic acid), pH and temperature not specified in the publication
140
NADPH
-
with 5,5'-dithiobis(2-nitrobenzoic acid) as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C
150
NADPH
-
in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication
398.5
NADPH
truncated mutant, pH 7.2, temperature not specified in the publication
1170
NADPH
-
pH 7.8, 37°C
1220
NADPH
pH 7.2, temperature not specified in the publication
75
thioredoxin
-
with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C
260
thioredoxin
-
with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C
1230
thioredoxin
-
in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication
52000
thioredoxin 2
-
mutant enzyme K137A, pH and temperature not specified in the publication
-
73000
thioredoxin 2
-
wild type enzyme, pH and temperature not specified in the publication
-
600
thioredoxin 41
-
with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C
-
880
thioredoxin 41
-
with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C
-
890
thioredoxin 8
-
with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C
-
950
thioredoxin 8
-
with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C
-
13.7
thioredoxin disulfide
-
Methanosarcina acetivorans thioredoxin 7, cosubstrate: NADPH, pH and temperature not specified in the publication
1900
thioredoxin disulfide
pH 7.0, 30°C
3700
thioredoxin disulfide
pH 7.0, 30°C
14920
thioredoxin disulfide
purified, recombinant, tetrameric enzyme
31700
thioredoxin disulfide
purified, recombinant, dimeric enzyme
41450
thioredoxin disulfide
mutant enzyme Y116I, in TE buffer, at 20°C
42150
thioredoxin disulfide
mutant enzyme Y116T, in TE buffer, at 20°C
65940
thioredoxin disulfide
wild type enzyme, in TE buffer, at 20°C