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1.8.1.9: thioredoxin-disulfide reductase

This is an abbreviated version!
For detailed information about thioredoxin-disulfide reductase, go to the full flat file.

Word Map on EC 1.8.1.9

Reaction

thioredoxin
+
NADP+
=
thioredoxin disulfide
 +
NADPH
 +
H+

Synonyms

all0737, ApTR, At2g41680, AtNTRA, AtNTRB, bacillithiol disulfide reductase, bacillithiol-disulfide reductase, BBOV_I002190, Bdr, BSSB reductase, CeTR2, DmTR, DmTrxR, DmTrxR-1, EC 1.6.4.5, EhTRXR, ferredoxin:thioredoxin reductase, FTR, general stress protein 35, GSP35, HCOI_01258400, hemolysate thioredoxin reductase, hemolysate TR, hTrxR, HvNTR1, HvNTR2, L-TR, L-TrxR, More, MtNTRC, mTR3, multifunctional thioredoxin-glutathione reductase, NAD(P)H:paraquat oxidoreductase, NADP-dependent thioredoxin reductase, NADP-linked thioredoxin reductase, NADP-thioredoxin reductase, NADP-thioredoxin reductase C, NADPH thioredoxin reductase, NADPH thioredoxin reductase C, NADPH-dependent thioredoxin reductase, NADPH-dependent thioredoxin reductase 2, NADPH-dependent thioredoxin reductase C, NADPH-dependent thioredoxin reductase I, NADPH-dependent thioredoxin reductase-1, NADPH-dependent TRX reductase, NADPH-thioredoxin reductase, NADPH-thioredoxin reductase C, NADPH-Trx reductase, NADPH2:oxidized thioredoxin oxidoreductase, NAPDH-dependent BSSB reductase, NTR, Ntr1, NTR2, Ntr3, NTRA, NTRAB, NtrB, NTRC, PH0178, PH1426, PhRP, PhTrxR, protein-disulfide oxidoreductase, reductase, thioredoxin, SEP1, taTrxR, TGR, thioredoxin glutathione reductase, thioredoxin h reductase, thioredoxin reductase, thioredoxin reductase (NADPH), thioredoxin reductase 1, thioredoxin reductase 2, thioredoxin reductase-1, thioredoxin reductase1, TON_1603, TR, TR1, TR2, TR3, TRase, TrR, Trr1, Trr1p, TRR2, Trx1, Trx3, TrxB, TrxB1, TrxB2, TrxR, TrxR-1(cyto), TrxR-1(mito), TrxR1, TrxR2, TrxR3, TrxRB3, TRXRD, TrxRh1, TrxRh2, TrxT, Txnrd1, TXNRD1-v3, Txnrd2, TXNRD3, TxrR-1, YpdA

ECTree

     1 Oxidoreductases
         1.8 Acting on a sulfur group of donors
             1.8.1 With NAD+ or NADP+ as acceptor
                1.8.1.9 thioredoxin-disulfide reductase

Crystallization

Crystallization on EC 1.8.1.9 - thioredoxin-disulfide reductase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
modeling of the structure of the NtrC dimer, extensive interactions between the Ntr domains and the Trx domains may stabilize the dimeric structure. A long linker region between the Ntr domain and Trx domain allows flexibility for the position of the Trx domain in the dimer
sitting drop vapor diffusion method at 4°C
sitting drop vapour diffusion method in the presence of 35% PEG 4000, 0.2 M ammonium acetate and citric acid buffer pH 5.1 at 25°C
-
hanging drop diffusion method at 20°C, X-ray crystal structure of DmTR at 2.4 A resolution in which the enzyme was truncated to remove the C-terminal tripeptide sequence Cys-Cys-Ser
crystal structures of thioredoxin reductase in complex with NADP+ in the oxidized and sodium dithionite-reduced form of the enzyme at 1.7 A and 1.45 A resolution, respectively
-
crystals of hTrxR1 are grown at 25°C by the hanging-drop technique. Crystal structure of hTrxR1 (Sec->Cys) in complex with FAD and NADP+ at a resolution of 2.8 A
in complex with FAD, NADPH and/or (N-acetyl-4-aminothiophenolato)(2,2':6',2''-terpyridine)platinum(II) chloride, sitting drop vapor diffusion method, using 5 mM MgSO4, 5% (w/v) PEG 3350, 50 mM MES pH 6.0 and 20% (w/v) 1,6-hexanediol
hanging drop vapor diffusion method, using 24% (w/v) PEG 400, 2% Jeffamine M-600, and 0.1 M citrate buffer pH 3.5
molecular modelling of the complex with thioredoxin
sitting drop vaour diffusion method at 25°C, high-resolution crystal structure of the apo-form of the enzyme at 2.6 A resolution
in the presence of 10 mM NADP+, hanging drop vapor diffusion method, using 15% polyethylene glycol 3350, 12% ethylene glycol, and 0.1 M HEPES, pH 7.5
microbatch-under-oil and hanging-drop vapour-diffusion
-
wild type and mutant enzymes with or without NADP+, using 12-15% (w/v) polyethylene glycol 2000 monomethyl ether, 0.1 M (NH4)2SO4, and 50 mM sodium acetate (pH 4.6)
wild type enzyme in complex with NADP+ and mutant enzyme C147A, using 12-15% (w/v) poly-ethylene glycol 2000 monomethyl ether, 0.1 M (NH4)2SO4 and 50 mM sodium acetate (pH 4.6)
hanging drop vapor diffusion method, using 0.1 M MES buffer, pH 6.5, 10% (w/v) PEG 20000, at 18°C
hanging drop vapor diffusion method, using 0.1 M MES buffer, pH 6.5, 10% PEG 20000, at 18°C
hanging-drop vapour diffusion in the presence of PEG 3000 as precipitant after treatment with hydrogen peroxide
-
hanging drop vapor diffusion method, using 22% (w/v) PEG 3350, 0.1 M HEPES pH 7.2, 0.2 M NaNO3, 5 mM dithiothreitol, or beta-mercaptoethanol
-
TGR alone or in complex with NADH and GSSG, sitting drop vapor diffusion method, using 0.1 M HEPES, pH 7.4, 20% PEG 3350, 0.2 M KI and 5 mM beta-mercaptoethanol or 0.2 M magnesium formate, 20% PEG 6000, 0.1 M Hepes, pH 7.0-7.5 and 5 mM beta-mercaptoethanol (TGR with reduced and ordered C-terminus)
2.35 A resolution structure
-