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1.8.1.4: dihydrolipoyl dehydrogenase

This is an abbreviated version!
For detailed information about dihydrolipoyl dehydrogenase, go to the full flat file.

Word Map on EC 1.8.1.4

Reaction

protein N6-(dihydrolipoyl)lysine
+
NAD+
=
protein N6-(lipoyl)lysine
 +
NADH
 +
H+

Synonyms

apicoplast E3, CDS, CIP50, coronin-interacting protein, dehydrogenase, lipoamide, dehydrolipoate dehydrogenase, DHLDH, DHLipDH, diaphorase, dihydrolipoamide dehydrogenase, dihydrolipoamide dehydrogenase E3, dihydrolipoamide:NAD+ oxidoreductase, dihydrolipoic dehydrogenase, dihydrolipomide dehydrogenase, dihydrolipoyl dehydrogenase, DLD, DLD1, Dld2, DLDH, DLDH dehydrogenase, DLDH diaphorase, DLDH2, DT-diaphorase, E3, E3 component, E3 component of 2-oxoglutarate dehydrogenase complex, E3 component of acetoin cleaving system, E3 component of alpha keto acid dehydrogenase complexes, E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes, E3 component of pyruvate complex, E3 lipoamide dehydrogenase, E3 protein component of 2-oxoacid dehydrogenase multienzyme complexes, E3 subunit of the alpha-ketoglutarate dehydrogenase complex, EC 1.6.4.3, Glycine cleavage system L protein, Glycine oxidation system L-factor, hDLDH, hE3, hLADH, L-protein, LAD, LADH, LDH, LDP-Glc, LDP-Val, LipDH, lipoamide dehydrogenase, lipoamide dehydrogenase (NADH), lipoamide dehydrogenase C, lipoamide dehydrogenase2, lipoamide oxidoreductase (NADH), lipoamide reductase, lipoamide-dehydrogenase-valine, lipoate dehydrogenase, lipoic acid dehydrogenase, lipoyl dehydrogenase, LPD, LPD-GLC, LPD-VAL, LPD1, LPD2, Lpd3, LpdA, LpdC, LpdG, LpdV, More, mtLPD2, NAD(P)H:lipoamide oxidoreductase, NADH dehydrogenase, NADH diaphorase, NADH:lipoamide oxidoreductase, nicotinamide adenine dinucleotide diaphorase, ORF-E3, pdhD, pdhL, PfaE3, rhDLDH, TAase, ubiquinone reductase

ECTree

     1 Oxidoreductases
         1.8 Acting on a sulfur group of donors
             1.8.1 With NAD+ or NADP+ as acceptor
                1.8.1.4 dihydrolipoyl dehydrogenase

Cloned

Cloned on EC 1.8.1.4 - dihydrolipoyl dehydrogenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
bfmBC gene encoding DLD. Ligated into vector pCR2.1, and introduced into Escherichia coli DH5alpha. Insert DNA recovered from the recombinant plasmid ligated into vector pET-28a(+), yielding pET-bfmBC and expressed in Escherichia coli BL21 (DE3)
dihydrolipoamide dehydrogenase component of the pyruvate dehydrogenase multienzyme complex, expression in Escherichia coli
-
DNA and amino acid sequence determination and analysis of both lipdh genes using RT-PCR, expression of the mitochondrial isozyme as GFP-fusion protein giving green fluorescence and of the apicoplast isozyme fused to acyl-carrier-protein resulting in red fluorescence, expression of truncated mitochondrial and apicoplastic isozymes lacking the putative target sequences as His-tagged proteins in Escherichia coli strain BL21-RIL(DE3), the apicoplast isozyme is expressed at very low levels
-
DNA and amino acid sequence determination and analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
-
expressed in Escherichia coli
expressed in Escherichia coli as a recombinant protein
-
expressed in Escherichia coli BL21 cells
-
expressed in Escherichia coli BL21(DE3)
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) Codon Plus RLI cells
expressed in Escherichia coli DH5alpha cells
expressed in Escherichia coli JM109 cells
expressed in Escherichia coli JM83 cells
expressed in Escherichia coli M109(lambdaDE3)
-
expressed in Escherichia coli M15 cells
-
expressed in Escherichia coli M15pREP4 cells
-
expressed in Escherichia coli Rosetta-gami (DE3) cells
-
expressed in Escherichia coli Rosetta-gami cells
-
expressed in Escherichia coli strain JRG 1342
-
expressed in Escherichia coli using the cytoplasmic expression vectors, pET3a and pET3d. Expressed as inclusion bodies and refolded by solubilisation in 8 M urea followed by dilution into a buffer containing 2 M KCl, 0.010 mM FAD, 1 mM NAD+, and 0.3 mM GSSG/3 mM GSH
expressed in Escherichia coli XL1-Blue
-
expression in a strain of Haloferax volcanii lacking dihydrolipoamide dehydrogenase activity
expression in Escherichia coli
expression in Escherichia coli or in Corynebacterium glutamicum, the cloned gene is expressed in Corynebacterium glutamicum cells harbouring the gene on a plasmid shows 12fold higher specific LPD activity when compared to the wild-type strain
expression in Escherichia coli strain JRG 1342
-
expression of D473L mutant in Escherichia coli strain XL1-Blue
-
expression of mutant T44V in Escherichia coli strain XL1-Blue
-
gene dld, DNA and amino acid sequence determination and analysis, genotyping
gene dld, located on chromosome 7, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain XL-1 Blue
gene dld, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain DH5alpha
gene dld, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain XL-1 Blue
gene DLD, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
gene encoding hDLDH but excluding the N-terminal 1?35 signal peptide region and containing an N-terminal His6-tag, recombinant expression in Escherichia coli strain BL21(DE3)
gene lpd, recombinant expression in Escherichia coli strain BL21(DE3)
-
gene lpd2, recombinant expression of GUS-linked enzyme in Arabidopsis thaliana ecotype Col-0 in cotyledons, rosette leaves and roots (in the cap of the established lateral roots, but not in the cap of the main roots) via transformation by Agrobacterium tumefaciens strain GV3101
gene lpd3, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene lpdG, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene lpdV, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene Pfae3, recombinant expression of His-tagged enzyme in Escherichia coli strain NovaBlue (DE3), transfection of pCC1-Pfae3 Plasmodium falciparum 3D7 erythrocytic stages
-
His-tagged wild-type and mutant proteins overexpressed in Escherichia coli
into vector pET101/D TOPO and expressed in Escherichia coli BL21(DE3)
-
ligated into vector pET-28a(+) and expressed in Escherichia coli BL21(DE3) as an N-terminal 6 x His-tag
mutants cloned into pQE-9 vector
Mycobacterium smegmatis over-expressing LpdC protein
-
overexpressed in the parent organism by using the halophilic archaeal rRNA promoter
overexpression in Escherichia coli
recombinant expression of N-terminally GST-tagged enzyme in Escherichia coli strain BL21(DE3)
wild-type and mutant enzymes K37E, H452Q and E457Q
-
wild-type and mutant enzymes K37E, H452Q and E457Q, overexpression in Escherichia coli
-
wild-type and mutant enzymes K54E, S53K54-K53S54 and E192Q, overexpression in Escherichia coli
-
wild-type DLD and mutant D444V expressed in Escherichia coli BL21 with an N-terminal six-histidine tag. C-term DLD, the interface domain residing the proteolytic activity of DLD, expressed in Escherichia coli without any tags
-