1.7.7.2: ferredoxin-nitrate reductase
This is an abbreviated version!
For detailed information about ferredoxin-nitrate reductase, go to the full flat file.
Reaction
Synonyms
assimilatory ferredoxin-nitrate reductase, Assimilatory nitrate reductase, C452_13229, dissimilatory nitrate reductase, EC 1.7.99.4, Fd-dependent nitrate reductase, Fd-NAS, NaR, narB, NarGH, NAS, NasB, nitrate (ferredoxin) reductase, nitrate reductase, reductase, nitrate (ferredoxin), respiratory nitrate reductase
ECTree
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KM Value
KM Value on EC 1.7.7.2 - ferredoxin-nitrate reductase
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0.0042
-
mutant enzyme K614R, pH and temperature not specified in the publication
0.0045
nitrate
-
mutant enzyme E219QR, pH and temperature not specified in the publication
0.006
nitrate
-
wild type enzyme, pH and temperature not specified in the publication
0.0066
nitrate
-
mutant enzyme R46K, pH and temperature not specified in the publication
0.007
nitrate
-
mutant enzyme R197Q, pH and temperature not specified in the publication
0.0081
nitrate
-
mutant enzyme R43K, pH and temperature not specified in the publication
0.0096
nitrate
-
mutant enzyme K201R, pH and temperature not specified in the publication
0.045
nitrate
120 mM Na2CO3 pH 9, 1 M NaCl, 4 mM methyl viologen, 35 mM KNO3, 17 mM Na2S2O4 (freshly prepared in 0.1 M NaHCO3) and 0.7 mU of enzyme preparations. The samples are incubated for 20 min at 60°C
0.055
nitrate
-
mutant enzyme R46Q, pH and temperature not specified in the publication
1.5
nitrate
-
reduced methyl viologen as electron donor, recombinant enzyme expressed in Escherichia coli
0.038
reduced ferredoxin
-
wild type enzyme, pH and temperature not specified in the publication
0.0419
reduced ferredoxin
-
mutant enzyme R197Q, pH and temperature not specified in the publication
0.0422
reduced ferredoxin
-
mutant enzyme E219QR, pH and temperature not specified in the publication
0.0445
reduced ferredoxin
-
mutant enzyme K614R, pH and temperature not specified in the publication
0.0456
reduced ferredoxin
-
mutant enzyme K201R, pH and temperature not specified in the publication
0.0486
reduced ferredoxin
-
mutant enzyme R43K, pH and temperature not specified in the publication
0.0496
reduced ferredoxin
-
mutant enzyme R46K, pH and temperature not specified in the publication
0.364
reduced ferredoxin
-
mutant enzyme R46Q, pH and temperature not specified in the publication
0.0646
reduced methyl viologen
120 mM Na2CO3 pH 9, 1 M NaCl, 4 mM methyl viologen, 35 mM KNO3, 17 mM Na2S2O4 (freshly prepared in 0.1 M NaHCO3) and 0.7 mU of enzyme preparations. The samples are incubated for 20 min at 60°C