1.7.7.1: ferredoxin-nitrite reductase

This is an abbreviated version!
For detailed information about ferredoxin-nitrite reductase, go to the full flat file.

Word Map on EC 1.7.7.1

Reaction

NH3
+ 2 H2O + 6 oxidized ferredoxin =
nitrite
+ 6 reduced ferredoxin + 7 H+

Synonyms

aNiR, assimilatory NiR, assimilatory nitrite reductase, CYME_CMG021C, CYME_CMJ117C, ferredoxin:nitrite oxidoreductase, ferredoxin:nitrite reductase, Nii1, nii3, nii4, NiR, nitrite reductase, NrfA, NrfH, reductase, ferredoxin-nitrite, SiRA, SirB

ECTree

     1 Oxidoreductases
         1.7 Acting on other nitrogenous compounds as donors
             1.7.7 With an iron-sulfur protein as acceptor
                1.7.7.1 ferredoxin-nitrite reductase

Crystallization

Crystallization on EC 1.7.7.1 - ferredoxin-nitrite reductase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
modeling of structure based on spinach nitrite reductase. Arginine and lysine residues are involved in electrostatically-stabilized binding to ferredoxin
the biological unit, NrfH2NrfA4, houses 28 c-type heme groups, 22 of them with low spin and 6 with pentacoordinated high spin configuration. The high spin hemes, which are the electron entry and exit points of the complex, carry a highly unusual coordination for c-type hemes, lysine and methionine as proximal ligands in NrfA and NrfH, respectively. The midpoint redox potential of the NrfH menaquinol-interacting methionine-coordinated heme is -270 mV; the biological unit, NrfH2NrfA4, houses 28 c-type heme groups, 22 of them with low spin and 6 with pentacoordinated high spin configuration. The high spin hemes, which are the electron entry and exit points of the complex, carry a highly unusual coordination for c-type hemes, lysine and methionine as proximal ligands in NrfA and NrfH, respectively. The redox potential of the catalytic lysine-coordinated high spin heme of NrfA is -50 mV
wild-type, to 1.25 A resolution and mutants Q448K, M175E, M175G, M175K to 2.0, 1.7. 1.7, 19 A resolution, respectively. The structure provides detailed geometries for the [4Fe–4S] cluster and the siroheme prosthetic groups
sitting drop vapour diffusion method
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NMR-study of protein-protein interaction of ferredoxin and nitrite reductase shows three acidic regions of ferredoxin to be major sites for the interaction with the enzyme, indicating that the complex is stabilized through electrostatic interaction
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