1.7.3.3: factor-independent urate hydroxylase
This is an abbreviated version!
For detailed information about factor-independent urate hydroxylase, go to the full flat file.
Word Map on EC 1.7.3.3
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1.7.3.3
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hyperuricemia
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peroxisomal
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xanthine
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allopurinol
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gout
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purine
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catalase
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allantoin
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biosensors
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electrode
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hematologic
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creatinine
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oxonic
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febuxostat
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allantoinase
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hyperkalemia
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hypoxanthine
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prophylaxis
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flavus
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hyperphosphatemia
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urate-lowering
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ureide
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methemoglobinemia
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pegylated
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amperometric
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uricosuric
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hypocalcemia
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tophi
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benzbromarone
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hominoid
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phosphotungstate
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microbodies
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hypouricemic
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probenecid
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utilis
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pimecrolimus
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pharmacology
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medicine
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analysis
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synthesis
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weight-based
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nodule-specific
-
miocene
-
drug development
- 1.7.3.3
- hyperuricemia
- peroxisomal
- xanthine
- allopurinol
- gout
- purine
- catalase
- allantoin
-
biosensors
-
electrode
-
hematologic
- creatinine
-
oxonic
- febuxostat
- allantoinase
- hyperkalemia
- hypoxanthine
-
prophylaxis
- flavus
- hyperphosphatemia
-
urate-lowering
-
ureide
- methemoglobinemia
-
pegylated
-
amperometric
-
uricosuric
-
hypocalcemia
-
tophi
- benzbromarone
-
hominoid
-
phosphotungstate
- microbodies
-
hypouricemic
- probenecid
- utilis
- pimecrolimus
- pharmacology
- medicine
- analysis
- synthesis
-
weight-based
-
nodule-specific
-
miocene
- drug development
Reaction
Synonyms
AaUO, AgUOX, dHU-wPU, ELITEK, Fasturtec, MVSM, N-35, Nodule specific uricase, Nodulin 35, Nodulin 35 homolog, Non-symbiotic uricase, oxidase, urate, Pucl, Rasburicase, Uaz, Uox, Urate oxidase, urate oxidoreductase, UriA, uric acid oxidase, uricase, uricase II, Uricoenzyme, Uricozyme
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pH Stability
pH Stability on EC 1.7.3.3 - factor-independent urate hydroxylase
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6 - 10
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the purified enzyme remains stable over a wide range of pH between 6.0-10.0 for 2 h
764830
7
UOX is deactivated at different protein concentrations at 45°C in 20 mM phosphate containing 0.15 M NaCl, 0.01 mg/ml UOX is deactivated much faster than its counterparts at concentrations of 0.1 and 1.0 mg/ml
700631
7 - 10
7 - 9.5
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in case of uricase entrapped in lipid vesicles, the remaining activity keeps more than 90% during the pH of 7.0-9.5, and the maximum remaining activity is 98.04% at pH 8.0 when incubated at 40°C or 40 min. For the free uricase, the maximum remaining activity is 86.59% at pH 8.5 when incubated at 40°C or 40 min
712197
7.4
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37°C, stable for 4 days, in presence of 30 microM oxonate stable for 22 days, with exponential decrease of activity thereafter
743752
9.2
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37°C, stable for 12 days, in presence of 30 microM oxonate stable for 22 days, with exponential decrease of activity thereafter
743752
additional information
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uricase activity in 50 h culture broth with pH values of 5.5 and 6.0 decreases more rapidly than that in cultures with pH values of 6.5 and 7.0, at pH 5.5, about 78% of initial uricase activity is lost within 25 h, under the same conditions, more than 85% of initial uricase activity remains in culture broth of pH 6.5 and 7.0, uricase activity in 66 h culture broth with pH 7.0 degrades much more rapidly than that in samples from 50 h culture, while for pH 6.5, the uricase is still stable, loss of uricase activity is caused by the degradation in acidic environment by proteases secreted by the host cells or releases from host cell lyses, low pH may cause instability of uricase
695795