1.7.3.3: factor-independent urate hydroxylase

This is an abbreviated version!
For detailed information about factor-independent urate hydroxylase, go to the full flat file.

Word Map on EC 1.7.3.3

Reaction

Urate
+
O2
+
H2O
=
5-hydroxyisourate
+
H2O2

Synonyms

AaUO, AgUOX, ELITEK, Fasturtec, N-35, Nodule specific uricase, Nodulin 35, Nodulin 35 homolog, Non-symbiotic uricase, oxidase, urate, Rasburicase, Uaz, Uox, Urate oxidase, urate oxidoreductase, UriA, uric acid oxidase, uricase, uricase II, Uricoenzyme, Uricozyme

ECTree

     1 Oxidoreductases
         1.7 Acting on other nitrogenous compounds as donors
             1.7.3 With oxygen as acceptor
                1.7.3.3 factor-independent urate hydroxylase

Temperature Stability

Temperature Stability on EC 1.7.3.3 - factor-independent urate hydroxylase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10 - 80
-
about 80% relative activity at 10°C, about 85% relative activity at 20-30°C, about 90% relative activity at 37°C, about 40% relative activity at 40°C, about 30% relative activity at 50°C, about 20% relative activity at 60°C, about 10% relative activity at 70°C, and no activity at 80°C after 30 min incubation
18 - 20
-
2 days, 70% loss of activity
37
-
12 h, 50% residual activity, complete loss of activity after 54 h
45
-
after 5 min 50% of initial activity
52
-
uricase covalently linked to monomethoxypoly(ethylene glycol) N-leucine-OSu-uricase and branched monomethoxypoly(ethylene glycol) N-leucine-OSu-uricase 50% loss of activity
55
-
uricase entrapped in lipid vesicles at enzyme concentrations of 0.005 and 0.1 mg/ml shows no loss of activity after 5 h at 55°C. Uricase entrapped in lipid vesicles at enzyme concentrations of 0.01 mg/ml shows about 35% loss of activity after 5 h at 55°C. Uricase entrapped in lipid vesicles at enzyme concentrations of 0.005 mg/ml shows about 50% loss of activity after 5 h at 55°C. The free uricase at 0.005 mg/ml is rapidly deactivated to about 30% of the initial activity within an incubation time of 2 h, while more than 70% of the initial enzyme activity remains for the uricase at 0.1 mg/ml in the identical incubation time
60
-
10 min, stable
60 - 61
-
native uricase and poly(N-acryloylmorpholine)-OSu-uricase 50% loss of activity
75
DQ887577
enzyme is thermostable, after heat treatment at 75°C for 45 min, the uricase retains about 100% of its initial activity
80
DQ887577
70% of initial activity remains after 45 min at 80°C
100
-
35% relative activity at 100°C
additional information