1.7.3.3: factor-independent urate hydroxylase
This is an abbreviated version!
For detailed information about factor-independent urate hydroxylase, go to the full flat file.
Word Map on EC 1.7.3.3
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1.7.3.3
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hyperuricemia
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peroxisomal
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xanthine
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allopurinol
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gout
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purine
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catalase
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allantoin
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biosensors
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electrode
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hematologic
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creatinine
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oxonic
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febuxostat
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allantoinase
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hyperkalemia
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hypoxanthine
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prophylaxis
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flavus
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hyperphosphatemia
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urate-lowering
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ureide
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methemoglobinemia
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pegylated
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amperometric
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uricosuric
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hypocalcemia
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tophi
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benzbromarone
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hominoid
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phosphotungstate
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microbodies
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hypouricemic
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probenecid
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utilis
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pimecrolimus
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pharmacology
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medicine
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analysis
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synthesis
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weight-based
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nodule-specific
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miocene
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drug development
- 1.7.3.3
- hyperuricemia
- peroxisomal
- xanthine
- allopurinol
- gout
- purine
- catalase
- allantoin
-
biosensors
-
electrode
-
hematologic
- creatinine
-
oxonic
- febuxostat
- allantoinase
- hyperkalemia
- hypoxanthine
-
prophylaxis
- flavus
- hyperphosphatemia
-
urate-lowering
-
ureide
- methemoglobinemia
-
pegylated
-
amperometric
-
uricosuric
-
hypocalcemia
-
tophi
- benzbromarone
-
hominoid
-
phosphotungstate
- microbodies
-
hypouricemic
- probenecid
- utilis
- pimecrolimus
- pharmacology
- medicine
- analysis
- synthesis
-
weight-based
-
nodule-specific
-
miocene
- drug development
Reaction
Synonyms
AaUO, AgUOX, dHU-wPU, ELITEK, Fasturtec, MVSM, N-35, Nodule specific uricase, Nodulin 35, Nodulin 35 homolog, Non-symbiotic uricase, oxidase, urate, Pucl, Rasburicase, Uaz, Uox, Urate oxidase, urate oxidoreductase, UriA, uric acid oxidase, uricase, uricase II, Uricoenzyme, Uricozyme
ECTree
1.7.3.3 factor-independent urate hydroxylaseAdvanced search results
results (
results (Inhibitors
Inhibitors on EC 1.7.3.3 - factor-independent urate hydroxylase
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INHIBITOR 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
beta-mercaptoethanol
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about 60% residual activity after 1 h incubation with 0.5 mM beta-mercaptoethanol at pH 8.5 and 25°C
Biguanidine salts
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inactivation is pH-dependent: slightly inhibitory below pH 10, rapid inactivation at high pH
D-sorbitol
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about 70% residual activity after 1 h incubation with 0.5 mM D-sorbitol at pH 8.5 and 25°C
Dicyandiamide
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inactivation is pH-dependent: small below pH 10, rapid increase at high pH
Guanidinium salts
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inactivation is pH-dependent: slightly inhibitory below pH 10, rapid inactivation at high pH
sodium deoxycholate
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about 90% residual activity after 1 h incubation with 0.5 mM sodium deoxycholate at pH 8.5 and 25°C
8-Azaxanthine
anion-pi interactions are present in the active site of the enzyme and are energetically favorable. Uric acid and 8-azaxanthine are able to interact favorably with cyanide and chloride ions, respectively and both uric acid and 8-azaxanthine react with water
CN-
the presence of residue Phe159 enhances the interaction energy of the anion with the urate pi system
Cu2+
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enzyme contains copper, inhibited by excessive addition of Cu2+, 98% inhibition by 1 mM CuCl2, 97% inhibition by 1 mM CuSO4
Cu2+
strong inhibition at 0.2 mM, negligible inhibition at 0.005 mM
EDTA
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enzyme activity is completely inhibited in presence of 5 mM EDTA
Fe2+
strong inhibition at 0.2 mM, negligible inhibition at 0.005 mM
H2O2
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purified uricase retains 72% of its original activity after incubation with 0.5% H2O2 for 6 h
Hg2+
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1 mM Hg2+ partially truncates enzyme activity to less than 50%
KCN
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enzyme activity is completely inhibited in presence of 5 mM KCN
SDS
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purified uricase retains 82% of its original activity after incubation with 0.5% SDS for 6 h
additional information
reduced levels of mRNA levels of urate oxidase after knock down of urate oxidase gene expression
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additional information
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reduced levels of mRNA levels of urate oxidase after knock down of urate oxidase gene expression
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additional information
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no inhibition by 1 mM Fe(ClO4)3 or 1 mM AgNO3
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additional information
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production of an egg yolk antibody specific to microbial uricase and its inhibitory effects on uricase activity
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additional information
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not inhibitory: Tween-20 or Triton X-100 at 0.5% w/v
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