1.7.2.6: hydroxylamine dehydrogenase

This is an abbreviated version!
For detailed information about hydroxylamine dehydrogenase, go to the full flat file.

Reaction

Synonyms

EC 1.7.3.4, HAO, haoA, HCR, HOA, hydroxylamine cytochrome c554 oxidoreductase, hydroxylamine oxidase, hydroxylamine oxidoreductase, hydroxylamine-cytochrome c reductase, hydroxylamine:oxygen oxidoreductase, mHAO, NeHAO, NirK, NoHAO

ECTree

     1 Oxidoreductases

         1.7 Acting on other nitrogenous compounds as donors

             1.7.2 With a cytochrome as acceptor

                1.7.2.6 hydroxylamine dehydrogenase

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Results	in table
8	Activating Compound
227	AA Sequence
1	Application
14	Cloned(Commentary)
67	Cofactor
7	Crystallization (Commentary)
6	Expression
6	General Information
1	General Stability
4	IC50 Value
42	Inhibitors
1	kcat/KM [mM/s]
1	Ki Value [mM]
24	KM Value [mM]
7	Localization
23	Metals/Ions
23	Molecular Weight [Da]
26	Natural Substrates/ Products (Substrates)
1	Organic Solvent Stability
113	Organism
2	Oxidation Stability
6	Pathway
12	PDB ID
13	pH Optimum
2	pH Range
1	pH Stability
7	Protein Variants
21	Purification (Commentary)
1	Reaction
86	Reference
30	Specific Activity [micromol/min/mg]
141	Substrates and Products (Substrate)
43	Subunits
110	Synonyms
1	Systematic Name
13	Temperature Optimum [°C]
2	Temperature Range [°C]
2	Temperature Stability [°C]
7	Turnover Number [1/s]

Purification

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Purification on EC 1.7.2.6 - hydroxylamine dehydrogenase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

3 entries

ammonium sulfate precipitation

Nitrosomonas europaea

-

394343

ammonium sulfate precipitation, DEAE cellulose column chromatography, gel filtration

Nitrosomonas europaea

-

394323

ammonium sulfate precipitation, DEAE-cellulose column chromatography, and Sephadex G-200 gel filtration

Nitrosomonas europaea

-

394315

ammonium sulfate precipitation, octyl-Sepharose column chromatography, and Sephacryl S-200 gel filtration

Nitrosomonas europaea

-

658900

ammonium sulfate precipitation, Sepharose CL-6B column chromatography, DEAE-Sephacel column chromatography, and Sephadex G-100 gel filtration

Nitrosomonas europaea

-

710728

ammonium sulfate precipitation, Sepharose CL-6B column chromatography, DEAE-Sephacel gel filtration, and Sephadex G—100 gel filtration

Nitrosomonas europaea

-

710728

ammonium sulfate precipitation, Sepharose CL-6B column chromatography, DEAE-Sephacel gel filtration, octyl Sepharose column chromatography, and Sephadex G-100 gel filtration

Nitrosomonas europaea

-

718820

DEAE Sefinose column chromatography and Sefinose CL-6B gel filtration

Acinetobacter sp. Y1

-

764339

maltose binding protein Trap column chromatography

4 entries

native enzyme from strain ATCC 19718 by anion exchange chromatography and gel filtration

Nitrosococcus oceani

-

741829

native enzyme from strain ATCC 19718 by anion exchange chromatography, gel filtration, and hdroxyapatite chromatography

Nitrosomonas europaea

Q50925

741829

partially purified by ammonium sulfate precipitation and DEAE column chromatography

Alcaligenes faecalis

-

764304

Sepharose CL-6B column chromatography and Sephacryl S-100 gel filtration

Bacillus sp. K5

-

765876

SP Sepharose column chromatography and Superdex 200 gel filtration

Methylacidiphilum fumariolicum

-

763649

-

Nitrosomonas europaea

-

657930, 658903

-

Nitrosomonas europaea

Q50925

720525

-

Nitrosococcus oceani

M5DCM0

725885

maltose binding protein Trap column chromatography

Campylobacter fetus

-

743303

maltose binding protein Trap column chromatography

Caminibacter mediatlanticus

-

743303

maltose binding protein Trap column chromatography

Nautilia profundicola

-

743303

maltose binding protein Trap column chromatography

Campylobacter curvus

-

743303