1.7.1.B3: aromatic nitroreductase [NADPH]

This is an abbreviated version, for detailed information about aromatic nitroreductase [NADPH], go to the full flat file.

Reaction

an aromatic amine
+ 3 NADP+ + 2 H2O =
an aromatic nitrate
+ 3 NADPH + 3 H+

Synonyms

ipa-43d, More, NADPH-FMN reductase, NADPH-nitrofurazone reductase, NfrA1, NfsA, nitro/flavin reductase, nitrofurazone reductase, nitroreductase A, non-luminescent-bacterial NfsA/Frp-type enzyme, oxygen-insensitive nitroreductase (NADPH)

ECTree

     1 Oxidoreductases
         1.7 Acting on other nitrogenous compounds as donors
             1.7.1 With NAD+ or NADP+ as acceptor
                1.7.1.B3 aromatic nitroreductase [NADPH]

Purification

Purification on EC 1.7.1.B3 - aromatic nitroreductase [NADPH]

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme 23fold from Escherichia coli to homogeneity by dialysis, anion exchange chromatography, dialysis, two steps of Blue Sepharose affinity chromatography, and again dialysis
-
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to over 95% purity
-