1.7.1.B2: aromatic nitroreductase [NAD(P)H]

This is an abbreviated version!
For detailed information about aromatic nitroreductase [NAD(P)H], go to the full flat file.

Reaction

+ 3 NAD(P)+ + 2 H2O =

+ 3 NAD(P)H + 3 H+

Synonyms

NfnB, NfsB, NfsB_Ec, NfsB_Pa, NprA, oxygen-insensitive nitroreductase (NAD(P)H), YfkO

ECTree

1 Oxidoreductases

1.7 Acting on other nitrogenous compounds as donors

1.7.1 With NAD⁺ or NADP⁺ as acceptor

1.7.1.B2 aromatic nitroreductase [NAD(P)H]

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Results	in table
4	AA Sequence
1	Application
7	Cloned(Commentary)
9	Cofactor
4	General Information
27	IC50 Value
8	Inhibitors
30	kcat/KM [mM/s]
32	KM Value [mM]
1	Molecular Weight [Da]
13	Natural Substrates/ Products (Substrates)
11	Organism
1	pH Optimum
20	Protein Variants
5	Purification (Commentary)
5	Reaction
11	Reference
33	Substrates and Products (Substrate)
1	Subunits
17	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
31	Turnover Number [1/s]

Purification

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Purification on EC 1.7.1.B2 - aromatic nitroreductase [NAD(P)H]

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His-bind Ni-NTA column chromatography

Escherichia coli

P38489

764166

Ni-NTA column chromatography

Escherichia coli

763904

Ni-NTA resin column chromatography

2 entries

recombinant wild-type and mutant N-terminally His6-tagged enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to over 95% purity

Escherichia coli

P38489

743709