1.7.1.B2: aromatic nitroreductase [NAD(P)H]
This is an abbreviated version!
For detailed information about aromatic nitroreductase [NAD(P)H], go to the full flat file.
Reaction
+ 3 NAD(P)+ + 2 H2O = + 3 NAD(P)H + 3 H+
Synonyms
NfnB, NfsB, NfsB_Ec, NfsB_Pa, NprA, oxygen-insensitive nitroreductase (NAD(P)H), YfkO
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General Information
General Information on EC 1.7.1.B2 - aromatic nitroreductase [NAD(P)H]
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evolution
nitroreductases are a family of flavoproteins that catalyze the NAD(P)H-dependent reduction of nitro groups to hydroxyamino and/or amino groups on vari-ous nitro-substituted compounds
physiological function
additional information
the enzyme crystal structure of NfsB, PDB ID 1DS7, is used for molecular ligand docking studies
Escherichia coli NfsB expressing HCT-116 cells are 23.5fold more sensitive to prodrug nitro-CBI-DEI than pure cultures of untransfected cells, monolayer cytotoxicity assays using human colon carcinoma HCT-116 cells. The discrepancy in the fold-sensitivity to nitro-CBI-DEI between the two and three dimensional cytotoxicity assays stems from loss of activated drug into the media in the monolayer cultures
physiological function
Pseudomonas aeruginosa NfsB expressing HCT-116 cells are 540fold more sensitive to prodrug nitro-CBI-DEI than pure cultures of untransfected cells, the activated drug that they generate also displays an unprecedented local bystander effect, monolayer cytotoxicity assays using human colon carcinoma HCT-116 cells. The discrepancy in the fold-sensitivity to nitro-CBI-DEI between the two and three dimensional cytotoxicity assays stems from loss of activated drug into the media in the monolayer cultures