1.7.1.B1: xenobiotic reductase B

This is an abbreviated version!
For detailed information about xenobiotic reductase B, go to the full flat file.

Reaction

Synonyms

flavoprotein reductase, old yellow enzyme, OYE, XenA, XenB, XenB protein, xenobiotic reductase A, xenobiotic reductase B, xenobiotic reductase B (XenB)-like flavin reductase

ECTree

     1 Oxidoreductases

         1.7 Acting on other nitrogenous compounds as donors

             1.7.1 With NAD⁺ or NADP⁺ as acceptor

                1.7.1.B1 xenobiotic reductase B

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Results	in table
2	AA Sequence
3	Cloned(Commentary)
3	Cofactor
2	Expression
5	Inhibitors
2	kcat/KM [mM/s]
13	KM Value [mM]
2	Metals/Ions
2	Molecular Weight [Da]
13	Organism
4	pH Optimum
4	Purification (Commentary)
12	Reaction
5	Reaction Type
10	Reference
4	Specific Activity [micromol/min/mg]
22	Substrates and Products (Substrate)
6	Subunits
27	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
2	Turnover Number [1/s]

Cloned

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Cloned on EC 1.7.1.B1 - xenobiotic reductase B

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

expressed in Escherichia coli Rosetta pLysS cells

Pseudomonas putida

-

765362

For His-tagged protein production, plasmid is transformed into Escherichia coli BL21 pLysS.

Pseudomonas putida

-

692112

xenB gene is amplified using appropriate primers with BamHI and HindIII sites and Pseudomonas putida KT2440 chromosomal DNA as a template. After digestion with restriction enzymes, the PCR product is ligated into the pET28b(
+) vector. Resulting plasmid contains the coding sequence in frame with a DNA sequence encoding a His-tag, which resulted in a hexahistidine tail. For protein-His6 expression, plasmid is transformed into Escherichia coli BL21.

Pseudomonas putida

Q88PD0

690550