1.7.1.17: FMN-dependent NADH-azoreductase
This is an abbreviated version!
For detailed information about FMN-dependent NADH-azoreductase, go to the full flat file.
Reaction
Synonyms
acpD, AZO, azo-dye reductase, AzoA, AzoR, AzoR1, azoreductase, AzoRo, AzrA, AzrG, flavin-containing oxygen-insensitive azoreductase, FMN dependent azoreductase, FMN-dependent methyl red reductase, FMN-dependent NADH-azo compound oxidoreductase, FMN-dependent NADH-azo reductase, FMN-dependent NADH-azoreductase, FMN-dependent-NADH azoreductase, indigo reductase, NADH-azoreductase
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Temperature Stability
Temperature Stability on EC 1.7.1.17 - FMN-dependent NADH-azoreductase
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10 - 40
purified recombinant enzyme, pH 7.0, 0.1 M sodium phosphate buffer, 30 min, stable at
20 - 100
purified recombinant wild-type enzyme, completely stable, while the mutant shows loss of 50% activity at about 55°C and inactivation at 90°C
65
additional information
the recombinant enzyme is an extremely stable indigo reductase, analysis of the structure of this highly thermostable indigo reductase and identification of the key amino acid residues responsible for the thermostability