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1.7.1.1: nitrate reductase (NADH)

This is an abbreviated version!
For detailed information about nitrate reductase (NADH), go to the full flat file.

Word Map on EC 1.7.1.1

Reaction

nitrite
+
NAD+
+
H2O
=
nitrate
+
NADH
+
H+

Synonyms

assimilatory NADH:nitrate reductase, Assimilatory nitrate reductase, cytosolic NADH nitrate reductase, EC 1.6.6.1, EC 1.7.99.4, MSMEG_4206, MSMEI_4108, NADH-dependent nitrate reductase, NADH-Nar, NADH-nitrate reductase, NADH-NO3- reductase, NADH:nitrate oxidoreductase, NaR, NaR1, NasA, NasC, NIA1, Nia2, nitrate reductase, NR, NR1, NR2, Pden_4449, reductase, nitrate

ECTree

     1 Oxidoreductases
         1.7 Acting on other nitrogenous compounds as donors
             1.7.1 With NAD+ or NADP+ as acceptor
                1.7.1.1 nitrate reductase (NADH)

Molecular Weight

Molecular Weight on EC 1.7.1.1 - nitrate reductase (NADH)

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100000
101000
2 * 101000
102000
2 * 102000
103000
-
2 * 103000, SDS-PAGE
110000
115000
-
2 * 115000, SDS-PAGE
144000
-
non-denaturing pore gradient PAGE
147000
-
gel filtration
180000
-
4 active fractions with MW of 91000 Da, 362000 Da, 180000 Da and 720000 Da are detected, gel filtration
20000
-
alpha2beta2gamma2molybdenum-containing-component, 2 * 40000 Da FAD-containing alpha-subunit + 2 * 20000 Da cytochrome b557 beta subunit + 2 *gamma subunit which carries molybdenum-containing component of 1000 Da
202000
205000
-
non-denaturing PAGE
220000
-
gel filtration
221000
-
sucrose density gradient centrifugation, gel filtration
225000
-
disc gel electrophoresis
230000
-
disc gel electrophoresis
270000
-
gel filtration, sucrose density gradient centrifugation
280000
-
equilibrium sedimentation
330000
-
gel filtration
356000
-
sucrose density gradient centrifugation, gel filtration
360000
-
equilibrium sedimentation
362000
-
4 active fractions with MW of 91000 Da, 362000 Da, 180000 Da and 720000 Da are detected, gel filtration
40000
-
alpha2beta2gamma2molybdenum-containing-component, 2 * 40000 Da FAD-containing alpha-subunit + 2 * 20000 Da cytochrome b557 beta subunit + 2 *gamma subunit which carries molybdenum-containing component of 1000 Da
440000
-
gel filtration
46000
-
1 * 46000 + 1 * 95000, SDS-PAGE
514000
-
gel filtration
55000
-
x * 55000, SDS-PAGE, His-tagged domain, x * 85000, SDS-PAGE, GST-tagged domain
720000
-
4 active fractions with MW of 91000 Da, 362000 Da, 180000 Da and 720000 Da are detected, gel filtration
85000
90000
91000
-
4 active fractions with MW of 91000 Da, 362000 Da, 180000 Da and 720000 Da are detected, gel filtration
95000
-
1 * 46000 + 1 * 95000, SDS-PAGE
98000
-
x * 98000, SDS-PAGE
additional information
-
the C-terminal 268 residues corresponding to the flavin-containing domain, amplified and expressed in E. coli show a MW of 30000 Da by SDS-PAGE