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1.7.1.1: nitrate reductase (NADH)

This is an abbreviated version!
For detailed information about nitrate reductase (NADH), go to the full flat file.

Word Map on EC 1.7.1.1

Reaction

nitrite
+
NAD+
+
H2O
=
nitrate
+
NADH
+
H+

Synonyms

assimilatory NADH:nitrate reductase, Assimilatory nitrate reductase, cytosolic NADH nitrate reductase, EC 1.6.6.1, EC 1.7.99.4, MSMEG_4206, MSMEI_4108, NADH-dependent nitrate reductase, NADH-Nar, NADH-nitrate reductase, NADH-NO3- reductase, NADH:nitrate oxidoreductase, NaR, NaR1, NasA, NasC, NIA1, Nia2, nitrate reductase, NR, NR1, NR2, Pden_4449, reductase, nitrate

ECTree

     1 Oxidoreductases
         1.7 Acting on other nitrogenous compounds as donors
             1.7.1 With NAD+ or NADP+ as acceptor
                1.7.1.1 nitrate reductase (NADH)

Inhibitors

Inhibitors on EC 1.7.1.1 - nitrate reductase (NADH)

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
14-3-3 protein BMH1
-
0.0018 mM, reduces activity to less than 20%, at pH 6.0
-
14-3-3 protein isoform chi
-
noncompetitive inhibition with a preferential binding to the substrate-bound state of the enzyme
-
14-3-3 protein isoform epsilon
-
noncompetitive inhibition with a preferential binding to the substrate-bound state of the enzyme
-
14-3-3 protein isoform kappa
-
noncompetitive inhibition with a preferential binding to the substrate-bound state of the enzyme
-
14-3-3 protein isoform lambda
-
noncompetitive inhibition with a preferential binding to the substrate-bound state of the enzyme
-
14-3-3 protein isoform ni
-
noncompetitive inhibition with a preferential binding to the substrate-bound state of the enzyme
-
14-3-3 protein isoform omega
-
noncompetitive inhibition with a preferential binding to the substrate-bound state of the enzyme
-
14-3-3 protein isoform phi
-
noncompetitive inhibition with a preferential binding to the substrate-bound state of the enzyme
-
14-3-3 protein isoform theta
-
noncompetitive inhibition with a preferential binding to the substrate-bound state of the enzyme
-
14-3-3B protein
-
-
-
2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone
-
a photosynthetic electron flux inhibitor of photosystem I, causes 61% inhibition at 0.008 mM
2-(4'-maleimidylanilino)naphthalene-6-sulfonic acid
2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide
-
a NO scavenger
3-(3,4-dichlorophenyl)-1,1-dimethylurea
adenine
-
inhibition of the recombinant FAD domain
adenosine
-
inhibition of the recombinant FAD domain
adenosine 5'-diphosphoribose
Aminooxyacetate
-
-
AMP
-
inhibition of the recombinant FAD domain
ATP
-
at low pH, the presence of ATP alone in the incubation medium is sufficient to inactivate nitrate reductase
azide
Bromophenol blue
Cucurbita sp.
-
noncompetitive versus NADH
Ca2+
-
5 mM, 90% inhibition of the low activity form, no activity of high activity form, inhibition is prevented by low concentrations of thiol compounds
Carbamoyl phosphate
-
competitive
Cd2+
-
0.002 mM, 40% inhibition, 10 mM EDTA protects up to 0.1 mM metal concentration
Cl-
-
alters the observed Mo(V) lineshape, mixed-type inhibitor, decreases both NADH:nitrate reductase and reduced methyl viologen:nitrate reductase activities
Co2+
-
1 mM, strong inhibition
Cr
-
after 24 h activity is reduced by 17.31%, 30.72% and 45% at 1 mM, 10 mM and 100 mM of Cr, respectively
Cr3+
-
0.002 mM, 20% inhibition, 10 mM nitrilotriacetic acid does not protect
Cr6+
-
0.001 mM, 62% inhibition, 10 mM nitrilotriacetic acid does not protect
Cu
-
after 24 h activity is reduced by 21.5%, 36% and 46% at 1 mM, 10 mM and 100 mM of Cu, respectively
Cyanate
-
-
cyanide
dicoumarol
-
competitive towards NADH
dithiothreitol
-
rate of inactivation is increased by NAD+, but not by NADP+
Fe2+
-
potent inhibitor, inhibition can be abolished by prior chelation of the metal by EDTA
Fe3+
-
less potent inhibitor, effect cannot reversed by EDTA
ferricyanide
-
inhibition of the recombinant FAD domain
ferrocytochrome c
-
inactivation in a biphasic reaction, immune to inactivation during turnover with nitrate
glutamine
-
1 mM, decrease of activity
hydroxylamine
KCN
-
0.1 mM, complete inhibition
menadione
-
inhibits nitrate reductase
methyl methanethiosulfonate
MgCl2
MoO42-
-
1 mM, strong inhibition
mutant G216S of 14-3-3A protein
-
-
-
N-ethylmaleimide
-
1 mM, complete inhibition
NADPH
-
nitrate protects
NaN3
-
0.1 mM, complete inhibition
Ni2+
-
up to 0.1 mM, not inhibitory, 1 mM, 20% residual activity
nicotinamide
-
inhibition of the recombinant FAD domain
nitrite
NMN
-
inhibition of the recombinant FAD domain
NO2-
-
10 mM, 65% inhibition
O-Methoxylamine
-
-
o-phenanthroline
-
1 mM, complete inhibition
p-chloromercuribenzoic acid
-
0.01 mM, complete inhibition
p-hydroxymercuribenzoate
phosphate
-
25 mM, increases activity with a nitrate concentration of 2 mM, decreases activity with a nitrate concentration of 0.1 mM
potassium ferricyanide
-
preincubation with potassium ferricyanide inactivates nitrate reductase
pyridoxal 5'-phosphate
sodium nitroprusside
-
a NO donor
thiocyanate
VO3-
-
1 mM, strong inhibition
Zn
-
after 24 h activity is reduced by 11%, 19% and 21% at 1 mM, 10 mM and 100 mM of Cr, respectively
additional information
-