1.7.1.1: nitrate reductase (NADH)
This is an abbreviated version!
For detailed information about nitrate reductase (NADH), go to the full flat file.
Word Map on EC 1.7.1.1
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1.7.1.1
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seedling
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molybdenum
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chlorophyll
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shoot
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biomass
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reductases
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denitrification
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ammonia
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neurospora
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denitrify
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maize
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chlorate
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alga
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tungstate
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crassa
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chlorella
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nidulans
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barley
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fumarate
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xanthine
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spinach
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urease
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nitrogenase
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dissimilatory
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viologen
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stomatal
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molybdate
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molybdoenzymes
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hydroponic
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chlamydomonas
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foliar
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molybdopterin
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gogat
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napa
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n2o
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nitrous
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isoniazid
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denitrificans
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paracoccus
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griess
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kno3
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mycorrhizal
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transpiration
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plumbaginifolia
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tungsten
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anammox
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ethambutol
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sausage
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6.3.1.2
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dinitrogen
- 1.7.1.1
- seedling
- molybdenum
- chlorophyll
- shoot
- biomass
- reductases
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denitrification
- ammonia
- neurospora
-
denitrify
- maize
- chlorate
- alga
- tungstate
- crassa
- chlorella
- nidulans
- barley
- fumarate
- xanthine
- spinach
- urease
- nitrogenase
-
dissimilatory
- viologen
-
stomatal
- molybdate
-
molybdoenzymes
-
hydroponic
- chlamydomonas
-
foliar
- molybdopterin
- gogat
-
napa
- n2o
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nitrous
- isoniazid
- denitrificans
- paracoccus
-
griess
- kno3
- mycorrhizal
-
transpiration
- plumbaginifolia
- tungsten
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anammox
- ethambutol
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sausage
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6.3.1.2
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dinitrogen
Reaction
Synonyms
assimilatory NADH:nitrate reductase, Assimilatory nitrate reductase, cytosolic NADH nitrate reductase, EC 1.6.6.1, EC 1.7.99.4, MSMEG_4206, MSMEI_4108, NADH-dependent nitrate reductase, NADH-Nar, NADH-nitrate reductase, NADH-NO3- reductase, NADH:nitrate oxidoreductase, NaR, NaR1, NasA, NasC, NIA1, Nia2, nitrate reductase, NR, NR1, NR2, Pden_4449, reductase, nitrate
ECTree
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Inhibitors
Inhibitors on EC 1.7.1.1 - nitrate reductase (NADH)
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14-3-3 protein BMH1
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0.0018 mM, reduces activity to less than 20%, at pH 6.0
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14-3-3 protein isoform chi
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noncompetitive inhibition with a preferential binding to the substrate-bound state of the enzyme
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14-3-3 protein isoform epsilon
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noncompetitive inhibition with a preferential binding to the substrate-bound state of the enzyme
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14-3-3 protein isoform kappa
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noncompetitive inhibition with a preferential binding to the substrate-bound state of the enzyme
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14-3-3 protein isoform lambda
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noncompetitive inhibition with a preferential binding to the substrate-bound state of the enzyme
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14-3-3 protein isoform ni
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noncompetitive inhibition with a preferential binding to the substrate-bound state of the enzyme
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14-3-3 protein isoform omega
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noncompetitive inhibition with a preferential binding to the substrate-bound state of the enzyme
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14-3-3 protein isoform phi
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noncompetitive inhibition with a preferential binding to the substrate-bound state of the enzyme
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14-3-3 protein isoform theta
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noncompetitive inhibition with a preferential binding to the substrate-bound state of the enzyme
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2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone
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a photosynthetic electron flux inhibitor of photosystem I, causes 61% inhibition at 0.008 mM
2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide
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a NO scavenger
ATP
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at low pH, the presence of ATP alone in the incubation medium is sufficient to inactivate nitrate reductase
Ca2+
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5 mM, 90% inhibition of the low activity form, no activity of high activity form, inhibition is prevented by low concentrations of thiol compounds
Cd2+
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0.002 mM, 40% inhibition, 10 mM EDTA protects up to 0.1 mM metal concentration
Cl-
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alters the observed Mo(V) lineshape, mixed-type inhibitor, decreases both NADH:nitrate reductase and reduced methyl viologen:nitrate reductase activities
Cr
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after 24 h activity is reduced by 17.31%, 30.72% and 45% at 1 mM, 10 mM and 100 mM of Cr, respectively
Cr3+
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0.002 mM, 20% inhibition, 10 mM nitrilotriacetic acid does not protect
Cr6+
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0.001 mM, 62% inhibition, 10 mM nitrilotriacetic acid does not protect
Cu
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after 24 h activity is reduced by 21.5%, 36% and 46% at 1 mM, 10 mM and 100 mM of Cu, respectively
dithiothreitol
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rate of inactivation is increased by NAD+, but not by NADP+
Fe2+
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potent inhibitor, inhibition can be abolished by prior chelation of the metal by EDTA
ferrocytochrome c
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inactivation in a biphasic reaction, immune to inactivation during turnover with nitrate
phosphate
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25 mM, increases activity with a nitrate concentration of 2 mM, decreases activity with a nitrate concentration of 0.1 mM
potassium ferricyanide
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preincubation with potassium ferricyanide inactivates nitrate reductase
Zn
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after 24 h activity is reduced by 11%, 19% and 21% at 1 mM, 10 mM and 100 mM of Cr, respectively
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a photosynthetic electron flux inhibitor of photosystem II, causes 51% inhibition at 0.001 mM
3-(3,4-dichlorophenyl)-1,1-dimethylurea
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inhibits the post-translational light activation of nitrate reducate
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0.001 mM, 76% inhibition, 10 mM EDTA protects up to 0.1 mM metal concentration
Cu2+
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potent inhibitor, inhibition can be abolished by prior chelation of the metal by EDTA
cyanide
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mechanism of reactivation of cyanide-inactivated nitrate reductase by flavins in light
cyanide
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reactivation by incubation with oxidant systems after inactivation by treatment with NADH and cyanide
cyanide
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inactivation by simultaneous presence of NADH and low concentrations of cyanide, reactivation by incubation with ferricyanide or by a short exposure to light in the presence of FAD
Ankistrodesmus braunii
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NO3-, cyanate, carbamoyl phosphate and azide protect from inactivation. Photoreactivation in presence of flavins, early inhibition appears to be competitive versus NO3-
hydroxylamine
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interacts with reduced cytochrome b557 during catalysis of the enzyme
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5 mM MgCl2 decreases enzyme activity at pH 7.5, this effect is completely reversed by the addition of EDTA into samples returning the enzyme's activity to its initial level
Mg2+
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5 mM, 70% inhibition of the low activity form, no inhibition of high activity form, inhibition is prevented by low concentrations of thiol compounds
Mg2+
native protein, highly sensitive to Mg2+, recombinant protein, not sensitive. Recombinant protein plus enzyme-free leaf extract of Ricinus communis shows restored high sensitivity to Mg2+, but remains unresponsive to ATP
NaCl
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100 mM NaCl reduces enzyme activity in leaves from plants grown on 10 mM nitrate and in roots from plants grown on 0.1 mM nitrate to about 50%
NAD+
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inactivation in presence of thiol compounds is enhanced by cyanide ions and can be reversed by ferricyanide
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inactivation in a biphasic reaction, immune to inactivation during turnover with nitrate
NADH
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p-hydroxymercuribenzoate causes the appearance of an FAD-requirement for inactivation by NADH of FMNH2-nitrate reductase
NADH
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conversion of the enzyme to a reduced inactive form, by preincubation with NADH, in absence of nitrate, occurs in presence of either dithiothreitol and/or FAD but not with cysteine. Pretreatment with NADH alone does not inactivate, a nucleophilic agent, i.e. cyanide or superoxide is necessary to inhibit electron transfer by the enzyme to nitrate
NADH
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0.01 mM NADH, in absence of nitrate, 50% loss of activity after 30 min, 0.05 mM nitrate prevents inactivation, 0.001 mM cyanide enhances degree of inactivation. Rapid reactivation after treatment with 0.3 mM ferricyanide or exposure to light, 230 mWatt per cm2, plus 0.02 mM flavin adenine dinucleotide
p-hydroxymercuribenzoate
Cucurbita sp.
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inactivation of NADH:nitrate reductase activity, no loss of bromphenol blue: nitrate reductase activity
p-hydroxymercuribenzoate
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inhibition of full and NADH-utilizing partial activities
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0.002 mM, 60% inhibition, 10 mM EDTA protects up to 0.1 mM metal concentration
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0.004 mM, 60% inhibition, 10 mM EDTA protects up to 0.1 mM metal concentration
Zn2+
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potent inhibitor, inhibition can be abolished by prior chelation of the metal by EDTA
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no enzyme inhibition by diphenyleneiodonium, i.e. DPI, a NADPH oxidase inhibitor, and poor inhibition by L-NG-monomethyl arginine citrate (L-NMMA), a NOS inhibitor. But nitrate reductase activity is sensitive to a decrease or increase of NO levels when NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide and NO donor sodium nitroprusside are added
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additional information
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increasing level of salinity causes decrease in enzyme activity
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additional information
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plant nitrate reductase-dependent mARC activity, NOFNiR, can catalyze NO production from nitrite in the presence of millimolar concentrations of nitrate, which strongly inhibits the NO producing nitrite reductase activity of the nitrate reductase
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additional information
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An inhibitory effect of Mg-ATP is cancelled in the presence of staurosporine (the protein kinase inhibitor) and completely reversed after addition of EDTA as well as AMP
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additional information
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inhibition by monospecific anti-nitrate reductase rabbit serum
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additional information
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purification of a NADH-nitrate reductase inhibitor from young leaves of Glycine max, that causes a reversible inhibition
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additional information
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the 14-3-3A protein and the 14-3-3C protein are functionally not capable to inhibit nitrate reductase
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additional information
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roots of seedlings from Oryza sativa contain a substance which inhibits the activity of nitrate reductase when NADH or FMNH2 is used as electron donor
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additional information
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inhibitor from primary and regenerated roots of nitrate-grown seedlings, main site of action is NADH:cytochrome c reductase component of the nitrate reductase, NADH protects
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additional information
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nitrate reductase inhibitor from root extract of rice seedlings. Inactivation proceeds in two steps: the inhibitor first binds with nitrate reductase to cause a reduction in both NADH:nitrate reductase and reduced benzyl viologen:nitrate reductase activity. In the second phase, there is a complete inactivation of NADH:nitrate reductase after about 20 min. Reduced benzyl viologen:nitrate reductase activity is not affected by the second phase of inactivation
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additional information
Pisum arvense
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maize root inactivating enzyme inactivates pea leaf nitrate reductase
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