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1.6.5.4: monodehydroascorbate reductase (NADH)

This is an abbreviated version!
For detailed information about monodehydroascorbate reductase (NADH), go to the full flat file.

Word Map on EC 1.6.5.4

Reaction

NADH
+
H+
+ 2 monodehydroascorbate =
NAD+
+ 2 ascorbate

Synonyms

12-oxophytodienoate reductase 3, AFR reductase, AFR-reductase, AFRR, ascorbate free radical reductase, ascorbate free-radical reductase, ascorbic free radical reductase, At1g63940, At3g09940, AtMDAR1, defensin, MDA, MDA reductase, MDAR, MDAR-OX, MDAR1, MDAR2, MDAR5, MDAsA reductase (NADPH), MDHA, MDHAR, MDHAR1, MDHAR2, MDHAR3, MDHAR6, metallothionein-like type 1 protein, monodehydroascorbate radical reductase, monodehydroascorbate reductase, monodehydroascorbate reductase 2, monodehydroascorbate reductase 4, More, MT-2, MT1K, NADH-semidehydroascorbate oxidoreductase, NADH:AFR oxidoreductase, NADH:ascorbate radical oxidoreductase, NADH:semidehydroascorbic acid oxidoreductase, NEC3, Nectarin III, OPR3, Os09g0567300, peroxisomal monodehydroascorbate reductase, PpMDHAR1, PpMDHAR2, PpMDHAR3, SDA reductase, semidehydroascorbate reductase, Solyc09g009390, SOR, SPD1, thioredoxin H2, Trx h2

ECTree

     1 Oxidoreductases
         1.6 Acting on NADH or NADPH
             1.6.5 With a quinone or similar compound as acceptor
                1.6.5.4 monodehydroascorbate reductase (NADH)

Cloned

Cloned on EC 1.6.5.4 - monodehydroascorbate reductase (NADH)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis
DNA and amino acid sequence determination and analysis, construction of a cDNA library, expression in Escherichia coli
expressed as GST-tagged enzyme in Escherichia coli BL21 cells
-
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells and in Nicotiana tabacum cv. petit havana
expressed in Nicotiana tabacum
expression in Escherichia coli
expression in Escherichia coli fused to maltose-binding protein
-
expression in Eschericia coli
expression of mitochondrial and chloroplastic isozymes as GFP-fusion proteins in transgenic Arabidopsis plants, targeting of recombinant fusion proteins to the organelles in vivo in transgenic plants, dual targeting is achieved by multiple transcription initiation sites, 2 transcripts, a small and a large one, both have an N-terminal extension
gene MDHAR3, functional overexpression in transgenic Solanum lycopersicum plant leaves, quantitative reverse-transcriptase PCR expression analysis
-
high level expression in Escherichia coli, using the T7 RNA polymerase expression system
-
overexpression in Escherichia coli
-
overexpression in Nicotiana tabacum. The transgenic plants exhibit up to 2.1fold higher monodehydroascorbate reductase activity and 2.2fold higher level of reduced ascorbate compared to non-transformed control plants. The transgenic plants show enhanced stress tolerance in term of significantly higher net photosynthesis rates under ozone, salt and polyethylene glycol stresses and greater PSII effective quantum yield under ozone and salt stresses
overproduced in Escherichia coli
quantitative reverse-transcription PCR expression analysis, functional recombinant enzyme overexpression driven by a CaMV double 35S promoter in Oryza sativa in three stable transgenic lines, MT22, MT24 and MT25 as single or multiple copy gene, using the Agrobacterium tumefaciens strain LBA4404 transfection method. All transgenic lines show better yield attributes such as a higher tiller number and increased 1000-grain weight compared to non-transgenics. They also show tolerance to salt at germination and seedling stages
-