1.5.99.B4: flavin-containing opine dehydrogenase

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For detailed information about flavin-containing opine dehydrogenase, go to the full flat file.

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Synonyms

BjOpnDH, bll7190, bll7191, bll7192, bll7193, flavin-containing opine dehydrogenase, Nox/Oox-like protein, OdhA, odhB, odhC, ooxA, OpnDH, SoxB

ECTree

1 Oxidoreductases

1.5 Acting on the CH-NH group of donors

1.5.99 With unknown physiological acceptors

1.5.99.B4 flavin-containing opine dehydrogenase

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Results	in table
3	Cloned(Commentary)
15	Cofactor
16	General Information
3	Inhibitors
13	kcat/KM [mM/s]
11	KM Value [mM]
3	Metals/Ions
8	Natural Substrates/ Products (Substrates)
8	Organism
4	pH Optimum
4	Protein Variants
3	Purification (Commentary)
1	Reaction
6	Reference
2	Specific Activity [micromol/min/mg]
14	Substrates and Products (Substrate)
9	Subunits
35	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
1	Temperature Stability [°C]
11	Turnover Number [1/s]

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Cloned on EC 1.5.99.B4 - flavin-containing opine dehydrogenase

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genes bll7191, bll7190, and bll7192, sequence comparisons and phylogenetic analysis, recombinant expression from vectors pETDuet-1, pACYCDuet-1, and pCOLADuet-1 with the (His)6-tag attached to the N-terminus of the bll7190 gene, whereas the S-tag is attached to the C-termini of the bll7191 and bll7192 genes, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)

Bradyrhizobium japonicum

Q89E96; Q89E94; Q89E97

742120

PpOdhB, PpOdhC, and PpOdhA genes in the PpOdhABC gene cluster, the genes encoding the beta-, gamma-, and alpha-subunits are arranged in tandem on the bacterial genome, genetic structure overview, and amino acid sequence determination, analysis, and comparisons, genetic structure, phylogenetic analysis, recombinant expression as N-terminally His6-tagged proteins in Escherichia coli strain BL21(DE3), recombinant expression of the PpOdhABC gene cluster in Pseudomonas putida

Pseudomonas putida

Q88EK6; Q88EK5

743588

the gene cluster from Bradyrhizobium japonicum consists of genes OdhB1-C-A-B2, from which two proteins, OdhAB1C and OdhAB2C, appear through the assembly of each beta-subunit together with common alpha- and gamma-subunits, and amino acid sequence determination, analysis, and comparisons, genetic structure, phylogenetic analysis, recombinant expression as N-terminally His6-tagged proteins in Escherichia coli strain BL21(DE3), recombinant expression of the PpOdhABC gene cluster in Pseudomonas putida

Bradyrhizobium japonicum

Q89E96; Q89E94; Q89E97

743588