1.5.99.B2: proline dehydrogenase (acceptor)

This is an abbreviated version!
For detailed information about proline dehydrogenase (acceptor), go to the full flat file.

Word Map on EC 1.5.99.B2

1.5.99.B2

delta1-pyrroline-5-carboxylate

prodh1

schizophrenia

pyrroline-5-carboxylate

trans-4-hydroxy-l-proline

hydroxyproline

medicine

Reaction

Synonyms

APE_1267.1, dye-linked L-proline dehydrogenase, L-proDH, L-proline dehydrogenase, L-proline: FAD oxidoreductase, L-proline:FAD oxidoreductase, LPDH, PDH, PDH1, PDH2, PF1246, PF1798, PH1364, PH1751, PIG6, POX, Pro dehydrogenase, PRODH, ProDH1, ProDH2, proline dehydrogenase, proline oxidase, prub, PutA, TK0117, TK0122, TPpdhB, TPpdhB2, TtProDH

ECTree

1 Oxidoreductases

1.5 Acting on the CH-NH group of donors

1.5.99 With unknown physiological acceptors

1.5.99.B2 proline dehydrogenase (acceptor)

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Results	in table
4	Activating Compound
13	AA Sequence
3	Application
27	Cloned(Commentary)
35	Cofactor
11	Crystallization (Commentary)
2	Disease/ Diagnostics
1	Expression
8	General Information
23	Inhibitors
8	kcat/KM [mM/s]
17	Ki Value [mM]
57	KM Value [mM]
23	Localization
3	Metals/Ions
36	Molecular Weight [Da]
10	Natural Substrates/ Products (Substrates)
155	Organism
2	Pathway
12	pH Optimum
4	pH Range
4	pH Stability
2	pI Value
28	Protein Variants
21	Purification (Commentary)
5	Reaction
65	Reference
2	Renatured (Commentary)
31	Source Tissue
9	Specific Activity [micromol/min/mg]
1	Storage Stability
125	Substrates and Products (Substrate)
33	Subunits
108	Synonyms
1	Systematic Name
17	Temperature Optimum [°C]
2	Temperature Range [°C]
12	Temperature Stability [°C]
34	Turnover Number [1/s]

Purification

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Purification on EC 1.5.99.B2 - proline dehydrogenase (acceptor)

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6 entries

DE52 anion exchange column chromatography

Pyrococcus furiosus

686651

DEAE Toyopearl 650M column chromatography and Superdex 200 gel filtration

Pyrobaculum calidifontis

724048

HisTrap column chromatography and Hitrap Q column chromatography

Deinococcus radiodurans

Q9RW55

724329

Ni-chelating column chromatography

4 entries

Ni-NTA column chromatography and Sephadex G-25 gel filtration

Thermus thermophilus

Q72IB8

687550

Ni2+ affinity column chromatography

Bacillus subtilis

Q8RMG1

687234

Ni2+ NTA affinity column chromatography

Pseudomonas putida

725126

Ni2+-charged chelating Sepharose column chromatography and Sephacryl S-300 gel filtration

Aeropyrum pernix

725476

recombinant C-terminally His6-tagged wild-type and mutant enzymes from Mycobacterium smegmatis mc24517 cells by nickel affinity chromatography

Mycobacterium tuberculosis

O50444

741885

recombinant enzyme

2 entries

recombinant enzyme via refolding, reconstitution and two-phase partitioning in an optimized aqueous two-phase system, containing 14% w/w PEG-1000 and 12% w/w Na2CO3 at pH 8.0, from Escherichia coli strain BL21 (DE3) plysS inclusion bodies in one step, method optimization and evaluation, overview

Pseudomonas fluorescens

742973