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1.5.99.B2: proline dehydrogenase (acceptor)

This is an abbreviated version!
For detailed information about proline dehydrogenase (acceptor), go to the full flat file.

Word Map on EC 1.5.99.B2

Reaction

L-proline
+
a quinone
=
(S)-1-pyrroline-5-carboxylate
 +
a quinol

Synonyms

APE_1267.1, dye-linked L-proline dehydrogenase, L-proDH, L-proline dehydrogenase, L-proline: FAD oxidoreductase, L-proline:FAD oxidoreductase, LPDH, PDH, PDH1, PDH2, PF1246, PF1798, PH1364, PH1751, PIG6, POX, Pro dehydrogenase, PRODH, ProDH1, ProDH2, proline dehydrogenase, proline oxidase, prub, PutA, TK0117, TK0122, TPpdhB, TPpdhB2, TtProDH

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.99 With unknown physiological acceptors
                1.5.99.B2 proline dehydrogenase (acceptor)

Cloned

Cloned on EC 1.5.99.B2 - proline dehydrogenase (acceptor)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in DLD-1.POX cells
-
expressed in Escherichia coli
expressed in Escherichia coli BL-21 (DE3) plysS cells
-
expressed in Escherichia coli BL-21 plysS (DE3) cells
-
expressed in Escherichia coli BL21 (DE3) cells
expressed in Escherichia coli BL21 (DE3) Codon Plus RILP cells
-
expressed in Escherichia coli BL21 (DE3) pLysS cells
-
expressed in Escherichia coli BL21(DE3) Codon Plus RIL cells
-
expressed in Escherichia coli BL21(DE3)pLysS cells
expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL and BL21-Gold(DE3)pLysS cells
expressed in Escherichia coli Rosetta(DE3)pLysS cells
-
expressed in H1299 cells
expression in Eshcerichia coli
N-terminal 8xHis tag
recombinant expression of the enzyme in Escherichia coli strain BL21 (DE3) plysS inclusion bodies
-
recombinant overexpression of C-terminally His6-tagged wild-type and mutant enzymes in Mycobacterium smegmatis mc24517 cells using vector pYUB1062
recombinant overexpression of wild-type and mutant MBP-fusion enzyme in Escherichia coli TOP 10 cells, MBP-TtProDH and MBP-clipped TtProDH are proneto aggregation through non-native self-association
semi-quantitative, and quantitative RT-PCR enzyme expression analysis
-
the two MsPDH genes MsPDH1 and MsPDH2 share a high nucleotide sequence homology and a similar exon/intron structure