1.5.99.4: nicotine dehydrogenase

This is an abbreviated version!
For detailed information about nicotine dehydrogenase, go to the full flat file.

Reaction

Synonyms

6-hydroxy-D-nicotine oxidase, 6-hydroxy-L-nicotine oxidase, D-nicotine oxidase, NctA, NDH, NdhAB, nicotine dehydrogenase, nicotine oxidase, nicotinic acid hydroxylase

ECTree

     1 Oxidoreductases

         1.5 Acting on the CH-NH group of donors

             1.5.99 With unknown physiological acceptors

                1.5.99.4 nicotine dehydrogenase

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Results	in table
1	Activating Compound
15	AA Sequence
1	CAS Registry Number
5	Cloned(Commentary)
23	Cofactor
1	Expression
10	General Information
19	Inhibitors
12	KM Value [mM]
8	Localization
13	Metals/Ions
8	Molecular Weight [Da]
12	Natural Substrates/ Products (Substrates)
40	Organism
5	Pathway
9	pH Optimum
4	Protein Variants
7	Purification (Commentary)
2	Reaction
3	Reaction Type
30	Reference
10	Source Tissue
9	Specific Activity [micromol/min/mg]
4	Storage Stability
48	Substrates and Products (Substrate)
6	Subunits
20	Synonyms
1	Systematic Name
6	Temperature Optimum [°C]
2	Turnover Number [1/s]

Cloned

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Cloned on EC 1.5.99.4 - nicotine dehydrogenase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

Paenarthrobacter nicotinovorans

Q93NH4

392431

expression in Escherichia coli

Agrobacterium tumefaciens

-

763949

gene 6hdno of pAO1, DNA and amino acid sequence determination and analysis, sequence comparisons, genetic organization

Paenarthrobacter nicotinovorans

Q59127, Q59128, Q59129

655850

gene 6hdno of pAO1, DNA sequence analysis, genetic organization, primer extension analysis, transcriptional analysis, binding and regulatory role of repressor HdnoR, effector protein IR1 is involved, overview

Paenarthrobacter nicotinovorans

Q59127, Q59128, Q59129

656187

gene nox, DNA and amino acid sequence deteremination and analysis, recombinant expression from a broad-host-range cloning vector in Escherichia coli strain DH5alpha and Pseudomonas putida strain KT2440, the enzyme is exxpressed in both hosts but only active in Pseudomonas putida

Pseudomonas sp.

M4T5L3

724032