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1.5.99.12: cytokinin dehydrogenase

This is an abbreviated version!
For detailed information about cytokinin dehydrogenase, go to the full flat file.

Word Map on EC 1.5.99.12

Reaction

N6-dimethylallyladenine
+
acceptor
+
H2O
=
adenine
+
3-methylbut-2-enal
+
reduced acceptor

Synonyms

AtCKX2, AtCKX3, AtCKX6, AtCKX7, CKO, CKO1, CKX, CKX1, CKX10, CKX12, CKX2, CKX2-2, CKX3, CKX4, CKX4a, CKX5, CKX6, CKX7, CKX8, CKX9, cytokinin dehydrogenase, cytokinin dehydrogenase 1, cytokinin dehydrogenase CKX1, cytokinin dehydrogenase CKX10, cytokinin oxidase, cytokinin oxidase/dehydrogenase, DhCKX, HvCKX1, isopentenyladenosine oxidase, More, N6-(D2-isopentenyl)adenosine oxidase, N6-dimethylallyladenine dehydrogenase, N6-isopentenylaldenine oxidase, OsCKX2, PsCKX1, PsCKX2, VIT_00s0252g00040, VIT_00s2520g00010, VIT_04s0008g01880, VIT_07s0005g05960, VIT_07s0005g06010, VIT_11s0016g02110, VIT_13s0158g00320, VIT_18s0001g13200, zeatin oxidase, ZmCKO1, ZmCKX1

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.99 With unknown physiological acceptors
                1.5.99.12 cytokinin dehydrogenase

Purification

Purification on EC 1.5.99.12 - cytokinin dehydrogenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation
High Q column chromatography
octyl Sepharose column chromatography
of recombinant protein
-
partial from leaves and calli of wild-type and transgenic plants
-
partially
-
partially, recombinant enzyme from Pichia pastoris
-
recombinant from yeast Yarrowia lipolytica by ammonium sulfate fractionation, gel filtration, and ion exchange chromatography
-
recombinant protein
recombinant protein from culture medium
-
recombinant vacuolar His-tagged isozyme AtCKX1 41.7fold from Pichia pastoris by ultrafiltration, hydrophobic interaction and hydroxyapatite chromatography, followed by nickel affinity chromatography and another step of hydrophobic interaction chromatography
recombinant vacuolar isozyme AtCKX3 18fold from Pichia pastoris by ultrafiltration, hydroxyapatite chromatography, nickel affinity chromatography, and anion exchange chromatography and another step of hydrophobic interaction chromatography
Sepharose 4B column chromatography