1.5.99.12: cytokinin dehydrogenase

This is an abbreviated version!
For detailed information about cytokinin dehydrogenase, go to the full flat file.

Reaction

Synonyms

AtCKX2, AtCKX3, AtCKX6, AtCKX7, CKO, CKO1, CKX, CKX1, CKX10, CKX12, CKX2, CKX2-2, CKX3, CKX4, CKX4a, CKX5, CKX6, CKX7, CKX8, CKX9, cytokinin dehydrogenase, cytokinin dehydrogenase 1, cytokinin dehydrogenase CKX1, cytokinin dehydrogenase CKX10, cytokinin oxidase, cytokinin oxidase/dehydrogenase, DhCKX, HvCKX1, isopentenyladenosine oxidase, More, N6-(D2-isopentenyl)adenosine oxidase, N6-dimethylallyladenine dehydrogenase, N6-isopentenylaldenine oxidase, OsCKX2, PsCKX1, PsCKX2, VIT_00s0252g00040, VIT_00s2520g00010, VIT_04s0008g01880, VIT_07s0005g05960, VIT_07s0005g06010, VIT_11s0016g02110, VIT_13s0158g00320, VIT_18s0001g13200, zeatin oxidase, ZmCKO1, ZmCKX1

ECTree

     1 Oxidoreductases

         1.5 Acting on the CH-NH group of donors

             1.5.99 With unknown physiological acceptors

                1.5.99.12 cytokinin dehydrogenase

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Results	in table
11	Activating Compound
2677	AA Sequence
1	Application
1	CAS Registry Number
36	Cloned(Commentary)
40	Cofactor
5	Crystallization (Commentary)
10	Expression
34	General Information
75	IC50 Value
65	Inhibitors
4	Ki Value [mM]
41	KM Value [mM]
40	Localization
9	Metals/Ions
43	Molecular Weight [Da]
73	Natural Substrates/ Products (Substrates)
134	Organism
2	Pathway
25	PDB ID
42	pH Optimum
1	pH Range
13	pI Value
18	Posttranslational Modification
10	Protein Variants
26	Purification (Commentary)
5	Reaction
3	Reaction Type
128	Reference
131	Source Tissue
26	Specific Activity [micromol/min/mg]
299	Substrates and Products (Substrate)
31	Subunits
168	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
1	Temperature Stability [°C]
28	Turnover Number [1/s]

Crystallization

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Crystallization on EC 1.5.99.12 - cytokinin dehydrogenase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

sitting-drop vapor diffusion method

Arabidopsis thaliana

Q9FUJ1

689937

hanging-drop vapor-diffusion method in the presence of N6-(buta-2,3-dienyl)adenine or N6-(penta-2,3-dienyl)adenine

Zea mays

Q9T0N8

688421

purified free recombinant enzyme, or purified recombinant enzyme complexed with trans-zeatin, N6-isopentenyladenine, and N6-benzyladenine, sitting drop vapour diffusion method, 20°C, protein solution: 30 mg/ml protein, 20 mM HEPES-KOH, pH 7.5, mixed with equal volume of well solution containing 0.1 M sodium acetat, pH 4.6, 0.2 M ammonium sulfate, and 12% w/v PEG 5000 monomethylether, a few hours, soaking in ligand solution and/or cryoprotectant 30% glycerol before data collection, X-ray diffraction structure determination and analysis at 1.7-2.0 A resolution, single isomorphous replacement, Fourier methods

Zea mays

Q9T0N8

656542

purified recombinant enzyme, 20 mg/ml protein with 0.5% w/v n-octyl-beta-D-glucopyranoside, hanging drop vapour diffusion method, 0.002 ml protein solution mixed with 0.002 ml reservoir solution containing 30% w/v PEG 1500, 0.1 M Tris-HCl, pH 7.0, 1 week, 20°C, X-ray diffraction structure determination and analysis at 1.95 A resolution

Zea mays

-

654108

wild type and L492A mutant enzymes, hanging drop vapor diffusion method

Zea mays

Q9T0N8

711322