1.5.3.13: N1-acetylpolyamine oxidase

This is an abbreviated version!
For detailed information about N1-acetylpolyamine oxidase, go to the full flat file.

Word Map on EC 1.5.3.13

Reaction

N1-acetylspermidine
+
O2
+
H2O
=
putrescine
+
3-acetamidopropanal
+
H2O2

Synonyms

APAO, AtPAO5, Bjpao2, HPAO, hPAO-1, More, MPAO, N1-acetyl polyamine oxidase, N1-acetylpolyamine oxidase, PAO, PAO5, PAOh1, polyamine oxidase, SelPAO5

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.3 With oxygen as acceptor
                1.5.3.13 N1-acetylpolyamine oxidase

Engineering

Engineering on EC 1.5.3.13 - N1-acetylpolyamine oxidase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H69E
compared with the wild-type enzyme the mutant enzyme shows lower Vmax value and higher Km-value
H69Q
compared with the wild-type enzyme the mutant enzyme shows lower Vmax value and higher Km-value
K315M
compared with the wild-type enzyme the mutant enzyme shows lower Vmax value and higher Km-value
T467S
compared with the wild-type enzyme the mutant enzyme shows lower Vmax value and higher Km-value
T467Y
compared with the wild-type enzyme the mutant enzyme shows lower Vmax value and higher Km-value
K315M
Y430F
mutation results in a 6fold decrease in the kcat value and the kcat/Km value for oxygen due to a comparable decrease in the rate constant for flavin reduction