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1.5.3.10: dimethylglycine oxidase

This is an abbreviated version!
For detailed information about dimethylglycine oxidase, go to the full flat file.

Word Map on EC 1.5.3.10

Reaction

N,N-dimethylglycine
+
5,6,7,8-tetrahydrofolate
 +
O2
=
sarcosine
 +
5,10-methylenetetrahydrofolate
 +
H2O2

Synonyms

dimethylglycine dehydrogenase, dmg, DMGO, DMGO1, DMGO2, N,N-dimethylglycine:oxygen oxidoreductase (demethylating)

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.3 With oxygen as acceptor
                1.5.3.10 dimethylglycine oxidase

Crystallization

Crystallization on EC 1.5.3.10 - dimethylglycine oxidase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
in complex with folate compounds. N-terminal region covalently binds FAD and has dimethylglycine dehydrogenase activity
-
mutant D552A in complex with tetrahydrofolate. Presence of one water molecule instead of the Asp552 side chain
-
purified recombinant wild-type enzyme, sitting-drop vapour diffusion method, 0.004 ml of protein solution containing 15 mg/ml protein, mixed with 0.002 ml reservoir solution containing 15% PEG 5000 monomethylether, 0.2 M MgCl2, and 0.1 M HEPES, pH 7.5, magnesium salt can be exchanged for acetate, for complexing the crystals are soaked in a solution containing folic or folinic acid in 15% PEG 5000 monomethylether and 0.3 M NaCl for 10 min, X-ray diffraction structure determination and analysis at 1.60 A resolution
-