1.5.3.1: sarcosine oxidase (formaldehyde-forming)
This is an abbreviated version!
For detailed information about sarcosine oxidase (formaldehyde-forming), go to the full flat file.
Word Map on EC 1.5.3.1
-
1.5.3.1
-
flavin
-
fad
-
heterotetrameric
-
n-methylglycine
-
creatininase
-
amidinohydrolase
-
n-methyltryptophan
-
corynebacterial
-
flavinylation
-
diagnostics
-
analysis
-
medicine
- 1.5.3.1
- flavin
- fad
-
heterotetrameric
- n-methylglycine
- creatininase
-
amidinohydrolase
- n-methyltryptophan
-
corynebacterial
-
flavinylation
- diagnostics
- analysis
- medicine
Reaction
Synonyms
heterotetrameric sarcosine oxidase, L-pipecolate oxidase, L-pipecolic acid oxidase, monomeric sarcosine oxidase, MSOX, PSO, sarcosine : oxygen oxidoreductase (demethylating), sarcosine oxidase, sarcosine: O2 oxidoreductase, sarcosine:oxygen oxidoreductase (demethylating), SO, SO-U96, SOX, SoxA, trd_1773, TSOX
ECTree
Advanced search results
Purification
Purification on EC 1.5.3.1 - sarcosine oxidase (formaldehyde-forming)
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
affinity-purification, 4-aminopyrrole-2-carboxylic acid as the affinity ligand, which is coupled with Sepharose CL 4B via spacers composed of epichlorohydrin and ethylenediamine. With the affinity medium, the purification process consists of only one affinity chromatography step to capture monomeric enzyme. The purified enzyme iss 94% and 96% pure when analyzed on an HPLC and reducing SDS-PAGE
HiTrap DEAE column chromatography, HisTrap column chromatography, HiTrap phenyl column chromatography, Resources Q column chromatography, and Superdex 200 gel filtration
-
native enzyme by ammoniumm sulfate fractionation, hydrophobic interaction and anion exchange chromatography, and gel filtration
-
recombinant His-tagged alpha and beta subunits from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity
-
recombinant protein, purified by one step of affinity purification on a Ni-Sepharose column, and another step of ion-exchange purification on a DEAE column