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1.5.1.B7: pseudopaline synthase

This is an abbreviated version!
For detailed information about pseudopaline synthase, go to the full flat file.

Reaction

pseudopaline
+
NADP+
+
H2O
=
(2S)-2-amino-4-([(1S)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino)butanoate
+
2-oxoglutarate
+
NADPH
+
H+

Synonyms

cntM, ODH, PA4835, PaODH, pseudopaline synthase

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.1 With NAD+ or NADP+ as acceptor
                1.5.1.B7 pseudopaline synthase

Crystallization

Crystallization on EC 1.5.1.B7 - pseudopaline synthase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant enzyme in apoform or in complex with substrates, products, and/or NADP+, hanging drop vapor diffusion method, mixing of 0.0015 ml of a 1.2 mg/ml protein solution with 0.0015 ml of well solution containing 100 mM Bis-Tris, pH 5.45, 200 mM ammonium acetate, and 24% PEG 3350, with NADP+, or 270-290 mM ammonium formate and 28% PEG 3350, with pseudopaline, 4°C, X-ray diffraction structure determination and analysis at 1.57-2.18 A resolution, molecular replacement and modelling
purified recombinant wild-type PaODH complexed with NADP+, X-ray diffraction structure determination and analysis, single wavelength anomalous dispersion, at 1.95 A resolution, molecular replacement and modelling