1.5.1.8: saccharopine dehydrogenase (NADP+, L-lysine-forming) This is an abbreviated version! For detailed information about saccharopine dehydrogenase (NADP+, L-lysine-forming), go to the full flat file .
Reaction
N6-(L-1,3-dicarboxypropyl)-L-lysine +
NADP+ +
H2O =
L-lysine +
2-oxoglutarate +
NADPH +
H+
Synonyms AasS, dehydrogenase, saccharopine (nicotinamide adenine dinucleotide phosphate, lysine-forming), L-lysine-alpha-ketoglutarate reductase, LKR, LKR/SDH, LOR, LOR-SDH, lysine 2-oxoglutarate reductase, lysine 2-oxoglutarate reductase-saccharopine dehydrogenase, lysine alpha-ketoglutarate reductase, lysine ketoglutarate reductase, lysine ketoglutarate/reductase saccharopine dehydrogenase, lysine-2-oxoglutarate reductase, lysine-alpha-ketoglutarate reductase, lysine-ketoglutarate reductase, lysine-ketoglutarate reductase/saccharopine dehydrogenase, lysine-ketoglutaric reductase, lysine:alpha-ketoglutarate:TPNH oxidoreductase (epsilon-N-[glutaryl-2]-L-lysine forming), nllkr/sdh, saccharopine (nicotinamide adenine dinucleotide phosphate, lysine-forming) dehydrogenase, saccharopine dehydrogenase, saccharopine dehydrogenase (NADP, lysine-forming), saccharopine dehydrogenase (nicotinamide adenine dinucleotide phosphate, lysine-forming)
ECTree
Purification
Purification on EC 1.5.1.8 - saccharopine dehydrogenase (NADP+, L-lysine-forming)
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1630.5fold purification of a bifunctional protein containing lysine-ketoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities
-
300fold purification, enzyme clearly separated from saccharopine dehydrogenase EC 1.5.1.9
-
44.6fold partial purification from liver
-
450fold purification, no separation of lysine-ketoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9
-
500fold purification of a single protein with lysine-ketoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities
-
9.6fold partial purification from liver
-
native enzyme partially through ammonium sulfate fractionation and desalting gel filtration
-
partial purification of a recombinant bifunctional protein with lysine-2-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities and of the monofunctional lysine-2-oxoglutarate reductase domain
-
partial purification of a single bifunctional protein with lysine-2-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities
-
partial purification of bifunctional enzyme with lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities
-
separation of lysine-ketoglutarate reductase and saccharopine dehydrogenase activities by limited proteolysis with elastase, chymotrypsin, and papain
-
single protein with lysine-ketoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities: aminoadipic semialdehyde synthase
-