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1.5.1.8: saccharopine dehydrogenase (NADP+, L-lysine-forming)

This is an abbreviated version!
For detailed information about saccharopine dehydrogenase (NADP+, L-lysine-forming), go to the full flat file.

Word Map on EC 1.5.1.8

Reaction

N6-(L-1,3-dicarboxypropyl)-L-lysine
+
NADP+
 +
H2O
=
L-lysine
 +
2-oxoglutarate
 +
NADPH
 +
H+

Synonyms

AasS, dehydrogenase, saccharopine (nicotinamide adenine dinucleotide phosphate, lysine-forming), L-lysine-alpha-ketoglutarate reductase, LKR, LKR/SDH, LOR, LOR-SDH, lysine 2-oxoglutarate reductase, lysine 2-oxoglutarate reductase-saccharopine dehydrogenase, lysine alpha-ketoglutarate reductase, lysine ketoglutarate reductase, lysine ketoglutarate/reductase saccharopine dehydrogenase, lysine-2-oxoglutarate reductase, lysine-alpha-ketoglutarate reductase, lysine-ketoglutarate reductase, lysine-ketoglutarate reductase/saccharopine dehydrogenase, lysine-ketoglutaric reductase, lysine:alpha-ketoglutarate:TPNH oxidoreductase (epsilon-N-[glutaryl-2]-L-lysine forming), nllkr/sdh, saccharopine (nicotinamide adenine dinucleotide phosphate, lysine-forming) dehydrogenase, saccharopine dehydrogenase, saccharopine dehydrogenase (NADP, lysine-forming), saccharopine dehydrogenase (nicotinamide adenine dinucleotide phosphate, lysine-forming)

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.1 With NAD+ or NADP+ as acceptor
                1.5.1.8 saccharopine dehydrogenase (NADP+, L-lysine-forming)

Cloned

Cloned on EC 1.5.1.8 - saccharopine dehydrogenase (NADP+, L-lysine-forming)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
bifunctional LKR/SDH cDNA and monofunctional LKR cDNA are cloned, expression in Saccharomyces cerevisiae mutant lys1, but LKR cannot complement yeast LKR null mutant because of its uni-directional catabolic activity, overexpression in wild type yeast
-
cloning of cDNA encoding the bifunctional protein with lysine 2-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities, expression of the single gene is subject to spatial and developmental controls, expression in Escherichia coli produces no active LKR, perhaps because of a missing post-translational modification in prokaryotes, expression in Saccharomyces cerevisiae mutant lys1
-
gene LKR/SDH, transcriptomic profiling of Arabidopsis thaliana under biotic and abiotic stresses. In Arabidopsis, a monofunctional SDH probably produced from the same gene encoding the bifunctional enzyme localizes in the mitochondria
gene lys1 encoding lysine 2-oxoglutarate reductase is cloned
-
gene nllkr/sdh, DNA and amino acid sequence determination and analysis, phylogenic analysis, quantitative real-time PCR expression analysis
genes encoding bifunctional protein with lysine 2-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities are cloned
isolation and cloning of the cDNA and single copy gene encoding bifunctional enzyme with lysine-ketoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9, no expression in Escherichia coli, gene product with amino domain corresponding to LKR and carboxy domain corresponding to SDH
-
isolation of the cDNA and gene encoding bifunctional enzyme with lysine-ketoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities
-