1.5.1.38: FMN reductase (NADPH)

This is an abbreviated version!
For detailed information about FMN reductase (NADPH), go to the full flat file.

Reaction

Synonyms

(NADPH)-dependent flavin mononucleotide reductase, (NADPH)-dependent FMN reductase, BC_1619, EC 1.5.1.29, EC 1.6.8.1, flavin reductase P, FMN reductase, FRP, More, NAD(P)H:FMN reductase, NADPH specific FMN reductase, NADPH-flavin oxidoreductase, NADPH-FMN oxidoreductase, NADPH:FMN oxidoreductase, NADPH:FMN reductase, SsuE, ssueE, ycbP, ydgI

ECTree

     1 Oxidoreductases

         1.5 Acting on the CH-NH group of donors

             1.5.1 With NAD⁺ or NADP⁺ as acceptor

                1.5.1.38 FMN reductase (NADPH)

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Results	in table
4073	AA Sequence
7	Cloned(Commentary)
16	Cofactor
3	Crystallization (Commentary)
11	General Information
9	Inhibitors
13	kcat/KM [mM/s]
28	KM Value [mM]
18	Molecular Weight [Da]
12	Natural Substrates/ Products (Substrates)
17	Organism
4	Pathway
31	PDB ID
8	pH Optimum
1	pH Range
10	Protein Variants
10	Purification (Commentary)
6	Reaction
17	Reference
1	Renatured (Commentary)
3	Specific Activity [micromol/min/mg]
2	Storage Stability
36	Substrates and Products (Substrate)
12	Subunits
42	Synonyms
1	Systematic Name
8	Temperature Optimum [°C]
1	Temperature Stability [°C]
8	Turnover Number [1/s]

KM Value

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KM Value on EC 1.5.1.38 - FMN reductase (NADPH)

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.007

2-thioFMN

Vibrio harveyi

-

pH 7.0, 23°C, enzyme derivative reconstituted from apoenzyme and 2-thioFMN

392300

0.0027 - 0.019

FAD

2 entries

0.000054 - 0.11

FMN

8 entries

0.5555

NADH

Escherichia coli

P80644

pH 7.9, 30°C, 0.0005 mM FMN

438770

0.003 - 0.2

NADPH

12 entries

0.025

riboflavin

Vibrio harveyi

-

pH 7.0, 23°C, native enzyme

392300

additional information

additional information

3 entries

-

0.0027

FAD

Escherichia coli

P80644

pH 7.9, 30°C

438770

0.019

FAD

Vibrio harveyi

-

pH 7.0, 23°C, native enzyme

392300

0.000054

FMN

Escherichia coli

P80644

pH 7.9, 30°C

438770

0.0005

FMN

Vibrio harveyi

-

pH 6.8, 23°C

392179

0.005

FMN

Vibrio harveyi

-

pH 7.0, 23°C, native enzyme

392300

0.008

FMN

Vibrio harveyi

-

pH 7.0, 23°C, single-enzyme spectrophotometric assay monitoring the NADPH oxidation

392200

0.014

FMN

Vibrio harveyi

-

pH 7.0, 23°C, spectrometric assay

392180

0.018

FMN

Vibrio harveyi

-

pH 5.6, 23°C, spectrometric assay

392180

0.08

FMN

Escherichia coli

P80644

pH 7.5, 25°C, recombinant mutant Y118F

764152

0.11

FMN

Escherichia coli

P80644

pH 7.5, 25°C, recombinant wild-type enzyme

764152

0.003

NADPH

Vibrio harveyi

-

pH 7.0, 23°C, enzyme derivative reconstituted from apoenzyme and 2-thioFMN

392300

0.009

NADPH

Vibrio harveyi

-

wild type enzyme, in 50 mM phosphate buffer, pH 7.0, at 22°C

724343

0.01

NADPH

Vibrio harveyi

-

mutant enzyme R225A, in 50 mM phosphate buffer, pH 7.0, at 22°C

724343

0.011

NADPH

Vibrio harveyi

-

pH 7.0, 23°C, native enzyme

392300

0.016

NADPH

Vibrio harveyi

-

mutant enzyme R133A, in 50 mM phosphate buffer, pH 7.0, at 22°C

724343

0.02

NADPH

Vibrio harveyi

-

pH 5.6, 23°C, spectrometric assay

392180

0.02

NADPH

Vibrio harveyi

-

pH 7.0, 23°C, single-enzyme spectrophotometric assay monitoring the NADPH oxidation

392200

0.034

NADPH

Vibrio harveyi

-

mutant enzyme N134A, in 50 mM phosphate buffer, pH 7.0, at 22°C

724343

0.04

NADPH

Vibrio harveyi

-

pH 7.0, 23°C

392180

0.04

NADPH

Vibrio harveyi

-

pH 7.0, 23°C, spectrometric assay

392180

0.046

NADPH

Escherichia coli

P80644

pH 7.9, 30°C, 0.003 mM FMN

438770

0.2

NADPH

Bacillus cereus

Q81BW8

with FMN, pH 7.2, 37°C

741865

additional information

additional information

Vibrio harveyi

-

the kinetic mechanism of FRP is changed to a sequential pattern with a Km(FMN) of 0.003 mM and a Km(NADPH) of 0.02 mM in a luciferase-coupled assay measuring light emission

392200

-

additional information

additional information

Escherichia coli

P80644

steady-state kinetic analysis of wild-type and mutant enzymes, kinetics of FMN binding, overview

741937

-

additional information

additional information

Escherichia coli

P80644

steady-state Michaelis-Menten kinetics, and rapid-reaction kinetic analyses of Y118A, DELTAY118 SsuE, and wild-type SsuE, as well as stopped-flow kinetics, overview

764152

-