1.5.1.31: berberine reductase

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For detailed information about berberine reductase, go to the full flat file.

Word Map on EC 1.5.1.31

1.5.1.31

alkaloid

berberis

columbamine

s-scoulerine

palmatine

somniferum

morphine

jatrorrhizine

isoquinoline

s-reticuline

methylenedioxy

dehydrogenate

flavoprotein

compartmentation

papaver

synthesis

Reaction

+ 2 NADP⁺ =

+ 2 NADPH +

Synonyms

(R)-canadine synthase, (S)-tetrahydroprotoberberine oxidase, STOX

ECTree

1 Oxidoreductases

1.5 Acting on the CH-NH group of donors

1.5.1 With NAD⁺ or NADP⁺ as acceptor

1.5.1.31 berberine reductase

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1	Application
1	Cloned(Commentary)
4	Cofactor
2	Natural Substrates/ Products (Substrates)
2	Organism
2	Pathway
1	pH Optimum
1	Protein Variants
1	Reaction
3	Reaction Type
2	Reference
1	Source Tissue
11	Substrates and Products (Substrate)
3	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]

Engineering

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Engineering on EC 1.5.1.31 - berberine reductase

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additional information

Berberis wilsoniae

recombinant coexpression of enzyme STOX and six other enzymes, including the three membrane-bound enzymes: the flavin-dependent oxidase berberine bridge enzyme, the cytochrome P450 canadine synthase, and a cytochrome P450 reductase, in Saccharomyces cerevisiae results in protoberberine alkaloid production from a simple benzylisoquinoline alkaloid precursor rac-norlaudanosoline. Improvement of flux through the pathway, by methods including enzyme variant screening, genetic copy number variation, and culture optimization, leading to an over 70fold increase in canadine titer up to 1.8 mg/l

743234