1.5.1.30: flavin reductase (NADPH)

This is an abbreviated version!
For detailed information about flavin reductase (NADPH), go to the full flat file.

Reaction

Synonyms

alkanesulfonate FMN reductase, ArsH, azoreductase, AZR�, biliverdin IXbeta reductase, biliverdin reductase B, biliverdin reductase-B, Biliverdin-IX beta-reductase, BLVRB, CysJ, EC 1.6.8.2, FAD-dependent NADP-reductase, flavin oxidoreductase, flavin reductase, flavin reductase 1, flavin reductase Nr1, flavin reductase P, FLR, FR1, Frb, Frd188, frd2, fre, Fre oxidoreductase, Fre-1, FRG/FRaseI, FRGvf, FRP, FRPvh, GHBP, Green heme binding protein, HpaC, ipa-43d, LuxG, More, NAD(P)H-dependent H2O2-forming flavin reductase, NAD(P)H:flavin-oxidoreductase, NADPH-dependant FMN reductase, NADPH-dependent diaphorase, NADPH-flavin oxidoreductase, NADPH-flavin reductase, NADPH-FMN oxidoreductase, NADPH-FMN reductase, NADPH-nitrofurazone reductase, NADPH-specific flavin reductase, NADPH:FAD oxidoreductase, NADPH:flavin oxidoreductase, NADPH:FMN oxidoreductase, NfrA1, nitro/flavin reductase, non-luminescent-bacterial NfsA/Frp-type enzyme, SsuE, TVAG_517010

ECTree

     1 Oxidoreductases

         1.5 Acting on the CH-NH group of donors

             1.5.1 With NAD⁺ or NADP⁺ as acceptor

                1.5.1.30 flavin reductase (NADPH)

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
7	Activating Compound
300	AA Sequence
1	Application
1	CAS Registry Number
24	Cloned(Commentary)
65	Cofactor
5	Crystallization (Commentary)
29	Disease/ Diagnostics
28	General Information
2	General Stability
36	Inhibitors
13	kcat/KM [mM/s]
8	Ki Value [mM]
64	KM Value [mM]
4	Localization
8	Metals/Ions
31	Molecular Weight [Da]
36	Natural Substrates/ Products (Substrates)
77	Organism
4	Pathway
31	PDB ID
13	pH Optimum
2	pH Range
2	pH Stability
2	pI Value
17	Protein Variants
24	Purification (Commentary)
12	Reaction
5	Reaction Type
59	Reference
2	Renatured (Commentary)
19	Source Tissue
11	Specific Activity [micromol/min/mg]
1	Storage Stability
129	Substrates and Products (Substrate)
31	Subunits
107	Synonyms
1	Systematic Name
10	Temperature Optimum [°C]
1	Temperature Range [°C]
3	Temperature Stability [°C]
27	Turnover Number [1/s]

Renatured

print visible entries

print all entries

Renatured on EC 1.5.1.30 - flavin reductase (NADPH)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide Go to Renatured Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

RENATURED/Commentary

ORGANISM

UNIPROT

LITERATURE

soluble denatured mutant enzyme E99K is mixed into 50fold volume of pre-cooled 50 mM phosphate buffer containing 0.1 M FMN and stirred on ice in the dark for 15 min for protein renaturation and the reconstitution of E99K holoenzyme

Vibrio harveyi

-

684711

the recombinant FlaR is insoluble under physiological conditions and its purification can be obtained under denaturing conditions in buffer supplemented with 8 M urea. Different refolding conditions are tested for their ability to renature the protein. Dialysis of the denatured protein against buffers at different pH levels reveals that rFlaR can be solubilized at below pH 8.0. The presence of cysteine-rich sequence suggests that the protein has a transitional metal-binding ability. Thus, rFlaR is dialyzed against PBS (pH 7.3) supplemented with 2 mM salts of either transition metal or calcium and magnesium (2 mM of each). rFlaR becomes fully soluble in the presence of 2 mM FeSO4. Partial solubility can be obtained in the presence of 2 mM CoCl2 or CuSO4

Streptococcus pneumoniae

A0A4M3JL10

765814